FERMENTE SUCUKTAN İZOLE EDİLEN ANTİLİSTERİAL ENTEROCOCCUS MUNDTII YB6.30 TARAFINDAN ÜRETİLEN BAKTERİYOSİNİN KARAKTERİZASYONU

Bu çalışmada, fermente sucuktan izole edilen antilisterial aktiviteye sahip Enterococcus mundtii YB6.30 tarafından üretilen bakteriyosinin karakterizasyonu amaçlanmıştır. YB6.30 izolatı Listeria türlerinin yanı sıra Staphylococcus aureus, Bacillus cereus, Salmonella Enteritidis, S. Typhimurium ve Escherichia coli gibi gıda patojenlerinin de dahil olduğu çeşitli Gram-pozitif ve Gram-negatif bakterileri inhibe etmiştir. Proteolitik enzim uygulaması sonucu YB6.30 izolatı tarafından üretilen antimikrobiyal maddenin bakteriyosin olduğu belirlenmiştir. E. mundtii YB6.30 suşunun tanısı 16S rRNA gen dizi analizi ve türe özgü primerler kullanılarak yapılan polimeraz zincir reaksiyonu (PZR) ile yapılmıştır. Farklı sıcaklık ve pH uygulamaları sonucu bakteriyosinin ısı stabil olduğu ve geniş pH aralığında (2.0-10.0) aktivite gösterdiği belirlenmiştir. Bakteriyosin vankomisin dirençli E. faecium ATCC 51559 suşuna karşı bakterisidal etki göstermiştir. PZR denemesi sonucu E. mundtii YB6.30’da mundtisin KS geni varlığı tespit edilmiştir. Trisin-SDS-PAGE analizi sonucu aktif protein bandının moleküler büyüklüğü yaklaşık 7.56 kDa olarak tespit edilmiştir.

CHARACTERIZATION OF BACTERIOCIN PRODUCED BY ANTILISTERIAL ENTEROCOCCUS MUNDTII YB6.30 ISOLATED FROM FERMENTED SUCUK

In this study, the characterization of bacteriocin produced by Enterococcus mundtii YB6.30 with antilisterial activity isolated from fermented sucuk was aimed. The YB6.30 inhibited various Gram-positive and Gram-negative bacteria, including Listeria species, as well as food pathogens such as Staphylococcus aureus, Bacillus cereus, Salmonella Enteritidis, S. Typhimurium and Escherichia coli. As a result of proteolytic enzyme treatment, the antimicrobial substance produced by YB6.30 was identified as bacteriocin. Identification of E. mundtii YB6.30 was done by 16S rRNA gene sequence analysis and polymerase chain reaction (PCR) using species-specific primers. As a result of different temperature and pH treatments, it was determined that bacteriocin is heat stable and shows activity in wide pH range (2.0-10.0). Bacteriocin showed a bactericidal activity against vancomycin resistant E. faecium ATCC 51559. As a result of the PCR experiment, the presence of mundtisin KS gene was detected in E. mundtii YB6.30. Molecular size of active protein band was determined as 7.56 kDa as a result of Tricine-SDS-PAGE analysis.

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Gıda-Cover
  • ISSN: 1300-3070
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1976
  • Yayıncı: Prof. Dr. İbrahim ÇAKIR
Sayıdaki Diğer Makaleler

MARGARİN FORMÜLASYONUNDA KONJÜGE LİNOLEİK ASİT KULLANIMININ DEPOLAMA STABİLİTESİ ÜZERİNE ETKİSİ

Ayşe GÜNEY, Hamza ALAŞALVAR, Hakan ERİNÇ

SAĞLIĞIN GELİŞTİRİLMESİ VE SÜRDÜRÜLEBİLİR BESLENME İÇİN ALTERNATİF BİR KAYNAK: YENİLEBİLİR BÖCEKLER

Mücahit MUSLU

GELENEKSEL YÖNTEMLE ÜRETİLEN OTLU PEYNİRLERİN BAZI KALİTE ÖZELLİKLERİNİN VE BİYOAKTİVİTESİNİN BELİRLENMESİ

Sümeyya KARA, Şenol KÖSE

MAÇA YEŞİL ÇAYI İLE ZENGİNLEŞTİRİLMİŞ BİTTER ÇİKOLATA GELİŞTİRİLMESİ

Mine ÖZGÜVEN, İjlal BERKTAŞ, Özlem GÖNÜL, Beraat ÖZÇELİK

BEYAZ VE TAM BUĞDAY UNLU EKMEK ÇEŞİTLERİNE EKLENEN BEYAZ DUT (Morus alba) YAPRAK VE POSASININ ANTİOKSİDAN VE ANTİDİYABETİK AKTİVİTE ÜZERİNE ETKİSİ

Ceren İNCE, Özlem ÇAĞINDI

HAŞHAŞ (Papaver somniforum) ÇEŞİTLERİNİN TOHUM YAĞLARININ YAĞ ASİDİ KOMPOZİSYONU, TOPLAM FENOLİK MADDE MİKTARI, ANTİOKSİDAN VE ANTİMİKROBİYAL AKTİVİTELERİ

İlkin YÜCELŞENGÜN, Ersin YÜCEL, Berna ÖZTÜRK, Gülden KILIÇ

TAHIL BARLARININ FARKLI BİYOPOLİMERLER İLE YENİLEBİLİR KAPLANMASI: DEPOLAMA SÜRESİNCE FİZİKSEL VE KİMYASAL ÖZELLİKLER ÜZERİNE ETKİSİ

Vildan EYİZ, İsmail TONTUL, Selman TÜRKER

FARKLI ORANLARDA MERCİMEK UNU VE PATATES NİŞASTASI İÇEREN KARIŞIMLARIN EKSTRÜDE ÇABUK ERİŞTE ÜRETİMİNDE KULLANIMININ İNCELENMESİ

Merve VATANSEVER, Burcu TIĞA, Seher KUMCUOĞLU, Şebnem TAVMAN

FERMENTE SUCUKTAN İZOLE EDİLEN ANTİLİSTERİAL ENTEROCOCCUS MUNDTII YB6.30 TARAFINDAN ÜRETİLEN BAKTERİYOSİNİN KARAKTERİZASYONU

Tuba ALTINKAYNAK, Yasin TUNCER

KAVUN ÇEKİRDEĞİ TOZUNUN ERİŞTENİN BAZI ÖZELLİKLERİNE ETKİSİ

İlyas ÇELİK, Kübra POZAN