Ayşe YEŞİLAYER, Sabriye BELGÜZAR, Hande Nur ASLAN

Domates Bakteriyel Benek ve Domates Bakteriyel Solgunluk Hastalıklarına Karşı Origanum onites L.’in Antibakteriyal Etkisi

Bu çalışma, Origanum onites L.’in uçucu yağ ve etanol ekstraktının Pseudomonas syringae pv. tomato (Pst) ve Clavibacter michiganensis subsp. michiganensis (Cmm) hastalık etmenlerine karşı antibakteriyal etkisini belirlemek amacıyla yürütülmüştür. Bitki materyali 2016 yılında Tokat ilinden toplanmıştır. İn vitro koşullar altında yürütülen çalışmada King B besi yeri kullanılmıştır. O. onites’in %10, 20, 30, 40 ve 50‘lik uçucu yağ konsantrasyonları filtre kağına emdirme yöntemi kullanılarak uygulanmıştır. Son konsantrasyon %1, 2, 3, 4 ve 8 olacak şekilde O. onites etanol ekstraktı otoklav edilmiş King B besi yerine karıştırılmıştır. Daha sonra 106 hücre/ml oranında hazırlanan Pst ve Cmm süspansiyonu uçucu yağ ve ekstrakt içeren besi yerlerine ekilmiştir. Uçucu yağ ve ekstraktın olmadığı besi yerleri negative kontrol olarak kullanılmıştır. Çalışma sonuçlarına göre, uçucu yağ ve ekstrakt konsantrasyonundaki artışa bağlı olarak O. onites’in patojenler üzerindeki etkisi de artmıştır. %50’lik O. onites uçucu yağ konsantrasyonu Pst ve Cmm gelişimini sırasıyla %73 ve %93 oranında engellemiştir. %8 konsantrasyondaki O. onites etanol ekstraktı ise Pst ve Cmm gelişimini sırasıyla %97 ve %99 oranında engellemiştir. Sonuç olarak, O. onites ekstraktının patojenler üzerindeki antibakteriyal etkisi, uçucu yağın etkisinden daha yüksek olarak belirlenmiştir.

Antibacterial Activity of Essential Oil and Extract of Origanum onites L. Against Bacterial Speck of Tomato and Tomato Bacterial Wilt Disease

This study was carried out to determine antibacterial activity of Origanum onites L., essential oiland ethanol extract, against Pseudomonas syringae pv. tomato (Pst) and Clavibacter michiganensis subsp.michiganensis (Cmm). Plant material was collected from Tokat province in 2016. The study was carried underin vitro conditions and used King B medium. At concentrations, 10, 20, 30, 40 and 50% of the essential oil ofO. onites was applied with method filter paper impregnation. The extract of O. onites was mixed withautoclaved King B medium to obtain final concentrations 1, 2, 3, 4 and 8%. And then 106 cell/ml of Pst andCmm suspensions were placed on the medium containing the essential oil and extract. The media withoutessential oil and extract were used as the negative controls. Based on the results of the study, increase in theessential oil and extract concentration resulted in increase of the efficacy on pathogens. At concentration, of50% essential oil of O. onites inhibited the growth of Pst and Cmm at a rate of 73% and 93%, respectively. Atconcentration of 8% extract of O. onites inhibited the growth of Pst and Cmm at a rate of 97% and 99%,respectively. In conclusion, it has been observed O. onites extract has higher antibacterial activity than essentialoil.

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Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi-Cover

ISSN: 1300-2910
Yayın Aralığı: Yılda 3 Sayı
Başlangıç: 1985
Yayıncı: Tokat Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi Yayın Ofisi