Ayşe KIZILYER, Şeniz DEMİRYÜREK, Mehmet Emin KALENDER, Serdar ÖZTUZCU, Ozan BALAKAN, Celaletdin CAMCI, Abdullah Tuncay DEMİRYÜREK

ROCK1 geni Lys222Glu ve Arg1262Gln polimorfizmleri ile meme kanseri arasındaki ilişkinin araştırılması

Bu çalışmanın amacı, Türk popülasyonunda ROCK1 gen polimorfizmleri ile meme kanserinin metastaz yapma özelliği arasındaki muhtemel ilişkiyi araştırmaktır. Bu araştırmada, ROCK1 geninin G15741A (Lys222Glu) ve G156064A (Arg1262Gln) polimorfizmleri için genotip dağılımlarını ve allel frekansını araştırmak ve bu polimorfizmler ile tümör metastazı arasındaki ilişkiyi ortaya koymak amaçlanmıştır. Bu çalışmada, en az 5 yıl süreyle metastaz gelişmemiş meme kanseri olan hastalar (nonmetastatik grup, n=92), metastaz gelişmiş meme kanserli hastalar (metastatik grup, n=101) ve herhangi bir hastalığı olmayan, kanser geçmişi olmayan, ilaç kullanmayan, sigara içmeyen ve alkol kullanmayan sağlıklı bayan gönüllüler (kontrol grubu, n=128) çalışmaya alındı. Hastalardan ve kontrol grubundan alınan kan örneklerinden DNA izole edildi ve LightCycler cihazıyla PCR yöntemi kullanılarak erime eğrisi analizi yapıldı. ROCK1 geni Lys222Glu ve Arg1262Gln polimorfizmlerinin kontrol grubu ile hasta grupları arasında genotip ve allel frekansları bakımından anlamlı fark gözlenmemiştir (P>0.05). Meme kanserinde ROCK1 gen polimorfizmlerinin rolünü araştıran bu ilk çalışmada, Lys222Glu ve Arg1262Gln polimorfizmlerinin meme kanseri gelişme riski veya metastazı ile ilişkili olmadığı bulunmuştur.

Investigation of the association between ROCK1 gene Lys222Glu ve Arg1262Gln polymorphisms and breast cancer

The purpose of this study was to evaluate the possible relationship between breast cancer metastasis and ROCK1 gene polymorphisms in Turkish population. In this study, investigation of genotypic distribution and allele frequency of ROCK1 genes G15741A (Lys222Glu) and G156064A (Arg1262Gln) polymorphisms, and studying the association between these polymorphisms and tumor metastasis were aimed. In the present study, we enrolled patients with breast cancer without metastasis at least 5 years (non-metastatic group, n=92), metastatic breast cancer patients (metastatic group, n= 101), and voluntary healthy women, with no disease and cancer history, no drug usage, no smoking and no alcohol abuse (control group, n=128). DNA was isolated from blood samples of patients and control group, and melting curve analyses were made with PCR method at LightCycler equipment. No significant differences in ROCK1 genes Lys222Glu and Arg1262Gln polymorphisms were observed between the control group and patients in terms of genotype and allele frequencies (P>0.05). In this research, which is the first study to evaluate the role of ROCK1 gene polymorphisms in breast cancer, no associations between the Lys222Glu and Arg1262Gln polymorphisms and risk of developing breast cancer or metastasis have been found.

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