Emine Gülçeri GÜLEÇ PEKER, K. Barbaros BALABANLI, Şule COŞKUN CEVHER, Meral EBEGİL

Evaluation of Serum Malondialdehyde And Nitric Oxide Levels in Patients with Cystic Echinococcosis

The aim of current study was to investigate the difference in the serum Malondialdehyde(MDA) and Nitric oxide (NO) levels between healthy individuals and patients with Cysticechinococcosis (CE). CE patients (201 person) and healthy individuals (160 person) as controlgroup were included in this study. Their MDA and NO levels were measured by usingspectrophotometric methods. MDA and NO levels were statistically significantly higher in theCE patients group than the healthy group (p < 0.05). The results indicate that oxidative stressmay have increased in CE patients as a consequence of immune response of the host.Considering results of this study, new approaches that related antioxidant therapies can bedeveloping with further studies in the diagnosis, control and treatment of CE disease.

PDF

___

Eckert, J. and Deplazes, P., “Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern”, Clinical Microbiology Reviews, 17(1): 107-135, (2004).
Altıntaş, N., “Past to present: echinococcosis in Turkey”, Acta Tropica, 85(2): 105-112, (2003).
Clark, I.A., Hunt, N.H. and Cowden, W.B., “Oxygen-derived free radicals in the pathogenesis of parasitic disease”, Advances in Parasitology, 25: 1-44, (1986).
Clark, I.A. and Rockett, K.A., “Nitric oxide and parasitic disease”, Advances in Parasitology, 37: 1- 56, (1996).
Klebanoff, S.J., “Myeloperoxidase: friend and foe”, Journal of Leukocyte Biology, 77(5): 598-625, (2005).
Kandemir, O., Eskandari, G., Camdeviren, H., Sahin, E., Kaya, A. and Atik, U., “Plasma malondialdehyde and nitrate levels in patients with brucellosis”, MEU Tıp Fakültesi Dergisi, 3: 405- 409, (2002).
McNeil, K.S., Knox, D.P. and Proudfoot, L., “Anti-inflammatory responses and oxidative stress in Nippostrongylus brasiliensis induced pulmonary inflammation”, Parasite Immunology, 24(1): 15-22, (2002).
Karaman, U., Celik, T., Kiran, T.R., Colak, C. and Daldal, N.U., “Malondialdehyde, Glutathione, and Nitric Oxide Levels in Toxoplasma gondii Seropositive Patients”, Korean Journal of Parasitology, 46(4): 293-295, (2008).
Kilic, E., Yazar, S., Saraymen, R. and Ozbilge, H., “Serum malondialdehyde level in patients infected with Ascaris lumbricoides”, World Journal of Gastroenterology, 9(10): 2332-2334, (2003).
Yazar, S., Kilic, E., Saraymen, R. and Ozbilge, H., “Serum malondialdehyde levels in patients infected with Plasmodium vivax”, West Indian Medical Journal, 53(3): 147-149, (2004).
Dormandy, T.L., “In praise of peroxidation.”, Lancet, 2(8620): 1126-1128, (1988).
Moncada, S. and Higgs, A., “The L-arginine-nitric oxide pathway”, The New England Journal of Medicine, 329(27): 2002-2012, (1993).
Colasanti, M., Gradoni, L., Mattu, M., Persichini, T., Salvati, L., Venturini, G. and Ascenzi, P., “Molecular bases for the anti-parasitic effect of NO”, International Journal of Molecular Medicine, 9(2): 131-134, (2002).
Beckman, J.S. and Koppenol, W.H., “Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and ugly”, American Journal of Physiology, 271(5 Pt 1): C1424-C1437, (1996).
Jefferies, J.R., Turner, R.J. and Barrett, J., “Effect of Fasciola hepatica excretory-secretory products on the metabolic burst of sheep and human neutrophils”, International Journal of Parasitology, 27(9): 1025-1029, (1997).
Abo-Shousha, S., Khalil, S.S. and Rashwan, E.A., “Oxygen free radical and nitric oxide production in single or combined human schistosomiasis and fascioliasis”, Journal of the Egyptian Society of Parasitology, 29(1): 149-156, (1999).
Touil-Boukoffa, C., Bauvois, B., Sancéau, J., Hamrioui, B. and Wietzerbin, J., “Production of nitric oxide (NO) in human hydatidosis: relationship between nitrite production and interferon-gamma levels”, Biochimie, 80(8-9): 739-744, (1998).
Zeghir-Bouteldja, R., Amri, M., Aitaissa, S., Bouaziz, S., Mezioug, D. and Touil-Boukoffa, C., “In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus”, Journal of Parasitology Research, doi: 10.1155/2009/624919, (2009).
Zeghir-Bouteldja, R., Amri, M., Bouaziz, S., Mezioug, D. and Touil-Boukoffa, C., “Comparative study of nitric oxide (NO) production during human hydatidosis: relationship with cystic fluid fertility”, Parasitology Research, 112(2): 649-654, (2013).
Green, L.C., Wagner, D.A., Glogowski, J., Skipper, .PL., Wishnok, J.S. and Tannenbaum, S.R., “Analyses of nitrate, nitrite and [15N] nitrate in biological fluids”, Analytical Biochemstry, 126(1): 131-138, (1982).
Miranda, K.M., Espey, M.G. and Wink, D.A., “A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite”, Nitric Oxide, 5(1): 62-71, (2001).
Casini, A., Ferrali, M., Pompelam, A., Maellaro, A. and Comborti, M., “Lipid peroxidation and cellular damage in extrahepatic tissues of bromobenzene-intoxicated mice”, American Journal of Pathology, 123(3): 520-531, (1986).
Ali, N.M., Ibrahim, A.N. and Ahmed, N.S., “Assessment of the effect of Allium sativum on serum nitric oxide level and hepatic histopathology in experimental cystic echinococcosis in mice”, Journal of Parasitic Disease, 40(3): 893-900, (2016).
Amri, M. and Touil-Boukoffa, C., “In vitro anti-hydatic and immunomodulatory effects of ginger and [6]-gingerol”, Asian Pacific Journal of Tropical Medicine, 9(8): 749-56, (2016).
Labsi, M., Khelifi, L., Mezioug, D., Soufli, I. and Touil-Boukoffa, C., “Antihydatic and immunomodulatory effects of Punica granatum peel aqueous extract in a murine model of echinococcosis”, Asian Pacific Journal of Tropical Medicine, 9(3): 211-20, (2016).
Kanazawa, T., Asahi, H., Hata, H., Mochida, K., Kagei, N. and Stadecker, M.J., “Argininedependent generation of reactive nitrogen intermediates is instrumental in the in vitro killing of protoscoleces of Echinococcus multilocularis by activated macrophages”, Parasite Immunology, 15(11): 619-623, (1993).
Steers, N.J., Rogan, M.T. and Heath, S., “In-vitro susceptibility of hydatid cysts of Echinococcus granulosus to nitric oxide and the effect of the laminated layer on nitric oxide production”, Parasite Immunology, 23(8): 411-417, (2001).
Ersayit, D., Kılıç, E., Yazar, S. and Artış, T., “Kistik ekinokokkozisli hastalarda oksidatif stres: oksidan ve antioksidan parametreler arasındaki ilişki”, Sağlık Bilimleri Dergisi, 18(3): 159-166, (2009).
Bakır, S., Çeliksöz, A., Çelik, V.K., Sarı, İ. and Aydın, H., “Antioxidant-oxidant status in patients with hydatid cyst”, Turkish Journal of Biochemstry, 37(1): 29-34, (2012).
Heidarpour, M., Mohri, M., Borji, H. and Moghdass, E., “Oxidative stress and trace elements in camel (Camelus dromedarius) with liver cystic echinococcosis”, Veterinary Parasitology, 187(3-4): 459-463, (2012).
Heidarpour, M., Mohri, M., Borji, H. and Moghdass, E., “Oxidant/antioxidant status in cattle with liver cystic echinococcosis”, Veterinary Parasitology, 195(1-2): 131-135, (2013).
Atambay, M., Karaman, U., Kiran, T.R., Colak, C., Karabulut, A.B, Celik, T. and Daldal, N., “Glutathione and malondialdehyde levels in patients with hydatid cyst”, Saudi Medical Journal, 29(1): 87-89, (2008).
Kılıç, E., Yazar, S., Başkol, G., Artış, T. and Ersayit, D., “Antioxidant and nitric oxide status in patients diagnosed with Echinococcus granulossus”, African Journal of Microbiology Research, 4(22): 2439-2443, (2010).
Kurkcuoglu, I.C., Ulas, T., Eser, I., Aydogan, H., Hazar, A., Sak, Z.H., Yalcin, F., Aydin, M.S., Demir, M.E. and Aksoy, N., “Evaluation of pre- and post-surgical oxidative stress parameters in patients with pulmonary cystic echinococcus”, European Review of Medical and Pharmacological Sciences, 16(4): 525-529, (2012).
Koltas, I.S., Yucebilgic, G., Bilgin, R., Parsak, C.K. and Sakman, G., “Serum malondialdehyde level in patients with cystic echinococcosis”, Saudi Medical Journal, 27(11): 1703-1705, (2006).