A Comparative Study on Fatty Acid Compositions, Total Phenolic Contents, and Antioxidant Potentials of Various Extracts from Different Parts of Euphorbia chamaesyce L.

A Comparative Study on Fatty Acid Compositions, Total Phenolic Contents, and Antioxidant Potentials of Various Extracts from Different Parts of Euphorbia chamaesyce L.

In this study, fatty acid compositions, total phenolic contents, and antioxidant effects of thestem and seed extracts from Euphorbia chamaesyce L. (Euphorbiaceae) were investigated.Linolenic and linoleic acids were found as the main fatty acid components in the seed and stemhexane extracts by using GC/MS. In the seed hexane extracts, linolenic and linoleic acids werefound as 32.42% and 15.04%, respectively. On the other hand, in the stem hexane extracts, theywere found as 22.31% and 14.25%, respectively. EC/SEM (seed methanolic extract, IC50 = 5.35± 0.01 µg/mL), EC/SEA (seed acetone extract, IC50 = 8.61 ± 0.11 µg/mL), and EC/SEE (seedethyl acetate extract; IC50 = 20.6 ± 1.12 µg/mL) showed higher radical scavenging effect thansynthetic antioxidant BHT. The results showed that the seeds of E. chamaesyce had a highamount of polyunsaturated fatty acids and remarkable antioxidant activities.

___

  • Vasas, A., Hohmann, J., “Euphorbia diterpenes: isolation, structure, biological activity, and synthesis (2008–2012)”, Chemical Reviews, 114: 8579-8612, (2014).
  • Genç, I., Kültür, Ş., “Euphorbia akmanii (Euphorbiaceae), a new species from Turkey", Phytotaxa, 265: 112-120, (2016).
  • Schmelzer, G.H., Gurib-Fakim, A., Plant resources of Tropical Africa, Vol. 11, Prota, Netherlands, pp. 475-477, (2008).
  • Tanaka, R., Kasubuchi, K., Kita, S., Tokuda, H., Nishino, H., Matsunaga, S., “Bioactive steroids from the whole herb of Euphorbia chamaesyce”, Journal of Natural Products, 63: 99-103, (2000).
  • Tanaka, R., Wada, S., Yamada, T., Yamori, T., “Potent antitumor activity of 3,4-seco-8betaH-Ferna4(23),9(11)-dien-3-oic acid (EC-2) and 3,4-seco-Oleana-4(23),18-dien-3-oic acid (EC-4), evaluated by an in vitro human cancer cell line panel”, Planta Medica, 72: 1347-1349, (2006).
  • Agarwal, R., Mustafa, J., Gupta, A., Osman, S., “Oil rich Euphorbiaceae seeds with high contents of linoleic acid”, Fett Wissenschaft Technologie, 97: 526-527, (1995).
  • Vogel, R., Pascual Villalobos, M., “Seed oils for new chemical applications. 1. Vernolic acid produced by Euphorbia lagascae”, Angewandte Botanik, 67: 31-41, (1993).
  • Eynard, A.R., “Potential of essential fatty acids as natural therapeutic products for human tumors”, Nutrition, 19: 386-388, (2003).
  • Kang, J.X., Leaf, A., “Prevention of fatal cardiac arrhythmias by polyunsaturated fatty acids”, The American Journal of Clinical Nutrition, 71: 202-207, (2000).
  • Lopez-Huertas, E., Baro, J., Carrero, J., Fonolla, J., Jimenez, J., Boza, J., “n-3 Fatty acids: health effects and opportunities to increase intake”, Agro Food Industry Hitech, 14: 18-21, (2003).
  • Gao, M., Xiao, H., “Activity-guided isolation of antioxidant compounds from Rhizophora apiculate”, Molecules, 17: 10675-10682, (2012).
  • Blois, M.S., “Antioxidant determinations by the use of a stable free radical”, Nature, 181: 1199- 1200, (1958).
  • Decker, E.A., Welch, B., “Role of ferritin as a lipid oxidation catalyst in muscle food”, Journal of Agricultural and Food Chemistry, 38: 674-677, (1990).
  • Oke-Altuntas, F., Aslim, B., Duman, H., Gulpinar, A. R., Kartal, M., “The relative contributions of chlorogenic acid and rutin to antioxidant activities of two endemic Prangos (Umbelliferae) Species (P. heynia and P. denticulata)”, Journal of Food Biochemistry, 39: 409-416, (2015).
  • Singleton, V., Rossi, J.A., “Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents”, American Journal of Enology and Viticulture, 16: 144-158, (1965).
  • Yegenoglu, H., Aslim, B. and Oke, F., “Comparison of antioxidant capacities of Ganoderma lucidum (Curtis) P. Karst and Funalia trogii (Berk.) Bondartsev & Singer by using different in vitro methods”, Journal of Medicinal Food, 14: 512-516, (2011).
  • Smallwood, I.M., Handbook of organic solvent properties, Butterworth-Heinemann, Oxford. (1996).
  • Dai, J., Mumper, R.J., “Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties”, Molecules, 15: 7313-7352, (2010).
  • Bruni, R., Muzzoli, M., Ballero, M., Loi, M.C., Fantin, G., Poli, F., Sacchetti, G., “Tocopherols, fatty acids and sterols in seeds of four Sardinian wild Euphorbia species”, Fitoterapia, 75: 50-61, (2004).
  • Ertas, A., Yilmaz, M. A., Firat, M., “Chemical profile by LC-MS/MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two Euphorbia species”, Natural Product Research, 29: 529-534, (2015).
  • Bell, S.J., Bradley, D., Forse, R.A., Bistrian, B.R., “The new dietary fats in health and disease”, Journal of the American Dietetic Association, 97: 280-286, (1997).
  • Innis, S.M., Friesen, R.W., “Essential n-3 fatty acids in pregnant women and early visual acuity maturation in term infants”, The American Journal of Clinical Nutrition, 87: 548-57, (2008).
  • Barla, A., Öztürk, M., Kültür, Ş., Öksüz, S., “Screening of antioxidant activity of three Euphorbia species from Turkey”, Fitoterapia, 78: 423-425, (2007).
  • Halliwell, B., Gutteridge, J., “Oxygen toxicity, oxygen radicals, transition metals and disease”, Biochemical Journal, 219: 1-14, (1984).
  • Farhan, H., Rammal, H., Hijazi, A., Daher, A., Reda, M., Annan, H., Chokr, A., Bassal, A., Badran, B., “Chemical composition and antioxidant activity of a Lebanese plant Euphorbia macroclada schyzoceras”, Asian Pacific Journal of Tropical Biomedicine, 3: 542-548, (2013).
  • Ashraf, A., Sarfraz, R. A., Rashid, M.A., Shahid, M., “Antioxidant, antimicrobial, antitumor, and cytotoxic activities of an important medicinal plant (Euphorbia royleana) from Pakistan”, Journal of Food and Drug Analysis, 23: 109-115, (2015).