Amechi Kingsley ANI, Felix EZEUGWU, Ebere OCHIN

Growth and Optimization Processes of Mixed Microbial Population Degrading Chrysene

The response surface methodology (RSM) of chrysene removal was studied using mixedmicrobial culture. The effects of process factors such as initial chrysene concentration, agitationrate and, pH on chrysene degradation were also investigated. Maximum chrysene degradationrates of 60 and 50% were achieved at 300 rpm agitation rate and pH 8, respectively. Chrysenerecoveries were within 51.22% to 72.12%. The numerical optimization process indicated thatthe optimum values for agitation rate, pH, and initial chrysene concentration were 334.3 rpm,8.8 and 40.6 mg/L, respectively, with the desirability of 0.955. However, the second orderquadratic model was statistically significant for the chrysene removal process with goodagreement between the R² (0.9642), adjusted R² (0.9320), and predicted R² (0.8289). Finally,the results obtained from this study highlighted the optimal conditions for chrysene removalfrom an aqueous medium.

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Das, N., Chandran, P., “Microbial degradation of petroleum hydrocarbon contaminants– an overview”, Biotechnology Research International, 11: 1–13, (2011).
Mrozik, A., Piotrowska-Seget, Z., Labuzek, S., “Bacterial degradation of polyaromatic Hydrocarbon”, Polish Journal of Environmental Studies, 12(1): 15–25, (2003).
Wild, S.R., Jones, K.C., “Polynuclear aromatic hydrocarbon in the United Kingdom environment: a preliminary study source inventory and budget”, Environmental Pollution, 88: 91–108, (1995).
Hadibarata, T., Tachibana, S., Itoh, K., “Biodegradation of chrysene an aromatic hydrocarbon by Polyporus sp. S133 in a liquid medium”, Journal Hazardous Material, 164: 911–917, (2009).
Gupte, A., Tripathi, A., Patel, H., Rudakiya, D., Gupte, S., “Bioremediation of polyaromatic hydrocarbon (PAH); A perspective”, The Open Biotechnol Journal, 10: 363–378, (2016).
Khan, F.I., Husain, T., Hejazi, R., “An overview and analysis of site remediation Technologies”, Journal of Environmental Management, 74: 95–122, (2004).
Haritash, A.K., Kaushik, C.P., “ Biodegradation aspect of polyaromatic hydrocarbon (PAHs) A review”, Journal of Hazardous Material, doi: 10.1016/j.jhazmat.2009.03.137, (2009).
Tam, N.F.Y., Guo, C.I., Yau, W.Y., Wang, Y.S., “Preliminary study on biodegradation of phenanthrene by bacteria isolated from mangrove sediments in Hong Kong”, Material Pollution Bulletin, 45: 316–324, (2002).
Boopathy, R., “Factors limiting bioremediation technology”, Bioresource Technology, 74: 63– 67, (2000).
Gailute, I., Rackauskiene, G., Grigiskis, S., “Changes in crude oil hydrocarbon degradation by Arthrobacter Sp. MI and Acinetobacter Pr82 in selected optimal condition”, BIOLOGIJA, 60: 134 -141, (2014).
Raina, M., Ian, M., Pepper, L., Gerba, P.C., “Review of Basic Microbiology concepts”, Elsevier Academic Press, p 3-7, (2008).
Yong-Chao, G., Shu-hai, G., Jia-Ning, W., Li, D., Wang, H., De Hui-Zang., “Effect of different remediation treatments on crude oil contaminated saline soil”, Chemosphere, 117: 486 – 493, (2014).
Roling, W.F., Milner, M.G., Jones, D.M., Lee, K.., Daniel, F., Swannell, R.J., Head, I.M., “Robust hydrocarbon degradation and dynamics of bacterial community during nutrient enhanced oil spill bioremediation”, Applied Environmental Microbiology, 68: 5537 – 5548, (2002).
Dave, B.P., Gheevariya. C.M., Bhatt, J.K., Dudhagara. D.R., Rajpara, R.K., “Enhanced chrysene degradation by a mixed culture Biorem-CGDB using response surface design”, Indian Journal of Experimental Biology, 53: 256–263, (2015).
Hadibarata, T., Tachibana, S., “Bioremediation of phenanthrene, chrysene and benzo[a]pyrene by Fungi screen from nature”, ITB Journal of Science, 41(2): 88–97, (2009).
Nayak, A.S., Sanganal, S.K., Mudde, S.K., Oblesha, A., Karegoudar, T.B., “A catabolic pathway for the degradation of chrysene by pseudoxanthomonas sp. PNK-04”, FEMS Microbiology Letters, 320: 128–134, (2011).
Pichiah, S., Pakshirajan, K., Saha, P., “Batch growth kinetics of an indigenous mixed microbial culture utilizing m-cresol as a sole source of carbon”, Journal of Hazardous Material, 162: 476 - 48, (2008).
Agu, C., Menkiti, M., Kadurumba, C., Menkiti, C., “Process parameter optimization for transformer oil extraction from Terminalia catappa seed using response surface methodology”, Journal of the Chinese Advanced Materials Society, 3(4): 328–344, (2015) doi: 10.1080/22243682.2015.1088794.
Deriase, S.F., Sherif, A., Younis, N., El-Gendy, S., “Kinetic evaluation and modeling for batch degradation of 2-hydroxybiphenel and 2, 2 dihydroxybiphenyl by Corynebacterium variabilis Sh42”, Desalination. Water Treatment, 51: 4719 – 4728, (2013) doi: 10.1080/19443994 2012. 744950.
Guntupalli, S., Thunuguntla, V.B.S.C., Rao, C.V., Bondili, J.S., “Microbial consortia of fungal and bacteria co-cultures increases biodegradation rate of polycyclic aromatic hydrocarbons”, International Journal Pharma Bio Sciences, 7(3): 562–570, (2016).
Asep, H., Sanro, T., “Simple screening for potential chrysene degrading fungi”, International Conference on Biological Science, (ICBS-2013) p 364–370 (2013).
Collina, E., Bestetti, G., Di Gennaro, P., Franzetti, A., Gugliersi, F., Lasagani, M., Pitea, D., “Naphthalene biodegradation kinetics in aerobic slurry – phase bioreactor”, Journal of Environmental International, 31: 167–171, (2005).
Banik, R.M., Singh, P., “Optimization of agitation and agitation rate for maximum production of L-Glutaminase by Bacillus cereus (MTCC 1305)”, VIVECHAN International Journal of Research 2: 14–25, (2011).
Shioya, S., Morikuwa, M., Kajihara, Y., Shimizu, H., “Optimization of agitation and aeration a condition for maximum virginiamycin production”, Applied Microbiology and Biotechnology, 51: 164 –169, (1999).
Feng, Y.Z., He, Z., Ong, S.L., Hu, J., Zhang, Z., Jen, N.W., “Optimization of agitation, temperature and aeration conditions for maximum β-manganese production”, Journal of Enzyme and Microbiology Technology, 32: 282–289, (2003).
Nigel, G., Cheng-Cheng, J., Xiang Zhong, L., Jia-Qian, J., Ma, J., “The Influence of pH on the degradation of phenol and chlorophenol by potassium ferrate”, Chemosphere, 56: 949–956, (2004).
European Commission, 96/23/EC COMMISSION DECISION of 12 August 2002 implementing council directive 96/23/EC concerning the performance of the analytical method and interpretation of result (notified under document number C(2002)3044)(Text with EEA relevance)(2002/657/EC), OJL-221(2002), 8 – 36.
European Commission, Commission Regulation (EC) No 333/2007, OJ 333(2007), 29-38.
Belo, R.F.C., Figueiredo, J.P., Nunes, C.M., Pissinatti, R., De Souza, S.V.C., Junqueira, R.G., “Accelerated solvent extraction method for the quantification of polycyclic aromatic hydrocarbon in cocoa beans by gas chromatography-mass spectroscopy”, Journal of Chromatography B, 1053: 87–100, (2017).
Swartz, E., Stockburger, L., Gundel, L.A., “Recovery of semi-volatile organic compounds during sample preparation: implications for characterization of airborne particulate matter”, Environ Sci Technol, 37: 597–605, (2003).
Raters, M., Matissek, R., “Quantification of polyaromatic hydrocarbon (PAHs) in cocoa and chocolate sample by HPLC-FD method”, Journal Agriculture Food Chemistry, 62: 10666–10671 (2014).
Shahavi, M.H., Morteza, H., Mohsen, J., Ghasem, N., “Optimization of encapsulated clove oil Particle size with the biodegradable shell using design expert methodology”, Pakistan Journal of Biotechnology, 12: 149–160, (2015).
Montgomery, C.D., “Design and analysis of experiments,” 8th Edition Hoboken, NJ; John Wiley & son (2013).
Bezerra, M.A., Santelli, R.E., Oliveria, E.P., Villar, L.S., Scaleria, L.A., “Response Surface methodology (RSM) Optimization, a tool in analytical chemistry”, Talanta, 76: 965– 977 (2008) doi; http//dx: 10.1016/J.talanta.2008.05.019.
Gani, P., Sunar, N., Peralta, H., Abdullatiff, A.A., Fatimah, M., Fuzi, S.Z.M., “Growth of microalgae Brotrycoccus sp in domestic wastewater and application of statistical design for the optimization of flocculation using alum and chitosan”, Preparative Biochemistry and Biotechnology, 47(4): 333–341, (2017).
Yang, L.S., Zhang, F., Ji, C.H., Hong, H., Deng, P., “Optimization of process parameter for Supercritical carbon dioxide extraction of passifora seed oil by response surface Methodology”, Journal of Supercritcal Fluid, 48: 9–14, (2009).
Debamista, N., Rajasimman, M., “Optimization and kinetics study on biodegradation of Atrazine using mixed microorganisms”, Alexandria Engineering Journal, 52: 499–505, (2013).