Ayçiçeği kabuğu hemiselülozik hidrolizatina adapte edilmiş Pichia stipitis mayası ile alkol üretimine zehir giderme ön işlemlerinin etkisi

Bu çalışmada, ayçiçeği tohum kabuğu hemiselüloz hidrolizatına adapte edilmiş Pichia stipitis mayası ile ayçiçeği tohum kabuğu hemiselülozik hidrolizatının etil alkol üretimi için, kullanım olanakları araştırılmıştır. Bu amaçla P.stipitis mayası hidrolizata adapte edilmiş ve daha sonra adapte edilmiş P.stipitis’in üç farklı ön işlemgörmüş hidrolizat ile fermantasyon performansı çalkalamalı kaplarda yapılan deneyler ile incelenmiştir. En yüksek alkol konsantrasyonu (9,51 g/L), en iyi verim ($Y_{P/S} 0,32 g g^{-1}$) ve en yüksek alkol üretim hızı (0,057 g /L saat), CaO ile aşırı titrasyon ön işlem görmüş hidrolizatın fermantasyonu ile elde edilmiştir. Benzer şekilde CaO ile aşırı titrasyon + sodyum sülfitle ön işlem görmüş hidrolizatın fermantasyonu ile de aynı alkol konsantrasyonuna (9,51 g/L) ulaşılmıştır.

The effect of detoxification pretreatments to alcohol production with adapted Pichia stipitis to sunflower seed hull hemicellulosic hydrolysate

In this study, the potential use of sunflower seed hull hemicellulosic hydrolysate for ethanol production with sunflower seed hull hemicellulosic hydrolysate adapted Pichia stipitis was investigated. For this purpose, P. stipitis was first adapted to hemicellulosic hydrolysate and then fermentation performance of P. stipitis with three hydrolysate which were subjected to different pretreatments was studied by fermentation experiments performed in shaking flasks. The highest concentration of alcohol (9.51g/L), best yield (0.32g/g) and maximum productivity (0.057 g/L h) were obtained with the fermentation of over-limed hydrolysate with CaO. Same alcohol concentration (9,51 g/L) was also achieved with the fermentation of hydrolysate treated with overlimed + sulfite addition.

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