Zahide Esra DURAK

ADENOZİN DEAMİNAZ ENZİMİNİN KİNETİK DAVRANIŞI ÜZERİNE ZERDEÇAL (CURCUMA LONGA) EKSTRESİNİN ETKİLERİNİN ARAŞTIRILMASI

Bu çalışmada, kanser metabolizmasının kilit enzimlerinden biri olan adenozin deaminaz (ADA) sığır karaciğerinden izole edilerek kısmen saflaştırılmış, kinetik özellikleri incelenmiş ve enzimin kinetik davranışı üzerine zerdeçal ekstresinin etkileri araştırılmıştır. ADA enziminin inhibitörsüz şartlarda çizilen Michaelis Menten ve Lineweaver Burk grafiklerinde V max değeri absorbans olarak 0,8 (spesifik aktivite olarak 40 mIU/g protein), Km değeri 100 mM olarak tespit edilmiştir. Zerdeçal ekstresinin iki farklı konsantrasyonunda çizilen Michaelis Menten ve Lineweaver Burk grafiklerinde ise her iki durum için Km değeri 100 mM olarak, V max değerleri ise % 10 zerdeçal konsantrasyonunda 0,5 (25 mIU/g protein), % 20 zerdeçal konsantrasyonunda ise 0,3 (15 mIU/g protein) olarak bulunmuştur. Bu sonuçlar zerdeçal bileşenlerinin ADA enzimini non-kompetetif türde inhibe ettiğini göstermektedir. ADA enziminin zerdeçal tarafından bu şekilde inhibisyonu, zerdeçalın anti kanser özelliklerine katkı sağlayan mekanizmalardan biri olabilir.

INVESTIGATION OF THE EFFECTS OF AQUOEUS TURMERIC (CURCUMA LONGA) EXTRACT ON KINETIC BEHAVIOR OF ADENOSINE DEAMINASE ENZYME

In this study, adenosine deaminase enzyme which plays an important role in cancer metabolism was isolated and partly purificated from cattle liver, and its kinetic properties were established with and without turmeric extract. V max and Km values of ADA enzyme were found to be 0.80 absorbance unit (as specific activity 40 mIU/g protein) and 100 mM respectively by using Michaelis Menten and Lineweaver Burk graphs drawn in the studies without inhibitor. In the Michaelis Menten and Lineweaver Burk graphs drawn with two different concentrations of Curcuma Longa extract, Km value was found to be 100 mM at both concentrations. V max value was found to be 0.5 (25 mIU/g protein) at the 10 % turmeric concentration,but it was 0.3 (15 mIU/g protein) at 20 % turmeric concentration. These results show that turmeric constituents inhibit ADA enzyme non competetively. This kind of inhibition of ADA enzyme by turmeric extract might be one of the mechanisms contributed to anti cancer potency of curcuma longa.

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