Civa-2-klorüre maruz kalmış aynalı sazan (Cyprinus carpio L., 1758) balıklarının karaciğer ve böbrek ince yapılarında gözlenen sitopatolojik değişmeler

Çalışmada 0.5ppm $HgCl_2$ dozuna 25 ve 50 gün süre ile maraz kalmış sazan balıklarının karaciğer ve böbrek dokulannda meydana gelen sitopatolojik değişmeler araştırılmıştır. Kontrol ve deneme gruplarına ait balıkların karaciğer ve böbrek numuneleri transmission elektron mikroskobunda incelenmiştir: Böbrek kesitlerinde nekrotik alanların görülmesi, civanın böbrek üzerine olan etkisinin karaciğere oranla daha fazla olduğu sonucunu doğurmuştur. Karaciğer dokusunda ilerlemiş nekroz yoktur. Ancak az miktarda elektronca yoğun madde çökelmelerinin meydana gelmesi ve lizozomal yapılarda artış izlenmesi ile civanın karaciğer hücrelerinde birikim yaparak etkili olduğu sonucuna varılmıştır. Zaman periyoduna bağlı olarak her iki organda da farklı bir dejenerasyon görülmediği gibi, uzun sürede dokularda rejenerasyon da tespit edilmemiştir.

Cyptopathological changes of mercury on the fine structures of liver and kidney of carps (Cyprinus carpio L., 1758)

Degenerative canges of mercury on the fine structures of liver and kidney of carps are described. Fish samples were exposed to the 0.5ppm doses of $HgCl_2$ for 25 days and 50 days periods. Than, the sections of liver and kidney of both test and control groups were examined by transmission electron microscope. It was observed that kidney had been degenerated much more than liver by mercury exposition. The kidney's degeneration was generally as necrotic areas on the tissues, however they were as electro-dense material and increasing of lysosomes on the cells of the liver. Cytopathologiçal deformations of liver and kidney were similar more or less on both tests groups of 25 and 50 days periods and any regeneration was not detected on the samples of 50 days-period.

___

  • 1.Güley , M. ve Vural, N., Toksikoloji., A.Ü. Eczacılık Fak. Yayınları, No 48, Ankara (1978).
  • 2.Clarkson, T.W., "Mercury", J. Amer. College Toxicol., 8, 1291-1295, (1989).
  • 3.Hodson, P. V., "The effect of metal metabolism on uptake, disposition and toxicity in fish", Aquat. Toxicol, 11, 3-18, (1988).
  • 4.Fowler, B. A., "Intracellular compartmentation of metals in aquatic organisms. Roles in mechanisms of cell injury", Environ. Health. Perspect., 7İ, 121-128, (1987). .
  • 5.Kim, J. H.,Birks, E. and Heisinger, F., "Protective action of selenium againts mercury in Northern Creek Chubbs", Bull. Environ. Contam. Toxicol., 17, 132-136, (1977).
  • 6.Heisinger, F., Hansen, C. D. and Kim, j. H. "Effect of selenium dioxide on the accumulation and toxicity of mercuric chloride in Goldfish", Arch. Environ. Contam. Toxicol., 8, 279-283, (1979).
  • 7.Duncan, D. A. and Klaverkamp, J. E. "Tolerance and resistance to cadmium in White Suckers (Cotastomus commerson) previously exposed to cadmium, mercury, zinc or selenium", Can. J. Fish Aqua. Sci., 40, 128-137, (1983).
  • 8.Anadu, D. I, Chapman, G. A., Curtis, L. R. at al., "Effect of zinc exposure on subsequent acute tolerance to heavy metals in Rainbow Trout" Bull. Environ. Cotam. Toxicol., 43, 329-336, (1989).
  • 9.Honda, K., Ichihashi, H. and Tatsukawa, R, "Tissue distribution of heavy metals and their variations with age, sex and their habitats in Japanese Serows {Capricornis crispus)", Arch. Environ. Contam. Toxicol., 16, 151-161, (1987).
  • 10.Pankakoski, E., Hyvarinen, H., Jalkanen, M. at al., "Accumulation of heavy metals in the Mole in Finland", Environ. Poll., 80, 9-16, (1993).
  • 11.Lewis, S. A., Becker, P. H. and Furness, R. W., "Mercury levels in eggs, tissue and feathers of Herring Gulls (Larus argentatus) from the German Wadden Sea coasts", Environ. Poll., 80, 293-299, (1993).
  • 12.Me Carter, J. A. and Roch, M., "Hepatic metallothionin and resistance to copper in juvenile Coho salmon", Comp. Biochem. Physiol., 74 (C), 133-137, (1983).
  • 13.Mistry, P., Lucier, W. and Fowler, B. A., "High affinity lead binding proteins in rat kidney cytsol mediate cell-free nuclear translation of lead", J. Pharmacol. Exp. Therap., 232, 462-469, (19859.
  • 14.Mistry, P., Mastri, C. And Fowler, B. A., "Influence of metals ions on renal cytsolic lead-binding proteins and nuclear uptake of lead in the kidney", Biochem. Pharmacol., 35, 711-713, (1986).
  • 15.Fowler, B. A. and Duval, G, "Effect of lead on the kidney: Roles of high affinity lead-binding proteins", Environ. Health Perspect., 91, 77-80, (1991).
  • 16.Conner, E. A. and Fowler, B. A., "Preliminary purification and partial characterisation studies of a low-molecular weight Cytsolic lead-binding protein in liver of the channel catfish {Ictalurus punctatus)", Aquat. Toxicol., 28, 29-36, (1994).
  • 17.Hibiya, T. (Ed.), Ân Atlas of Fish Histology. Normal and Pathological Features, Gustav Fischer Verlang Stuttgart, New York, (1982).
  • 18.George, S. G., "Heavy metal detoxication in Mytilus kidney: An in vitro study of Cd and Zn binding to isolated tertiary lysosome", Comp. Biochem. Physiol., 76(C), 59-65,(1983).
  • 19.Nicholson, J. K., Kendall, M. D. and Osborn, D., " Cadmium and mercury nephrotoxicity", Nature, 304, 633-635, (1983).
  • 20.Roncero, V., Gazqeez, E., Redondo, E. at al., "Etude structurale et ultrastructurale du rein la Tanche Tinea tinea L., apres intoxication experimentale par absortion de nitrate de Plomb", J. Vet. Med. A.,35, 529-543, (1988).
  • 21.Kirubagaran, R. and Joy, K. P., "Toxic effects of three mercurial compounds on survivial and histology of the kidney of the Catfish (Clarias batrachus L.)", Ecotoxicol. Environ. Safety, 15,171-179, (1988).
  • 22. Wester, P. W. and Canton, J. H. "Histopathological effect in Poecitta reticulata (Guppy) exposed to methyl chloride", Toxicol. Pathol., 20, 81-91, (1992).