Enis MACİT, Şahan SAYGI, Neslihan GÜRBÜZ, Erdal CİLA

The determination of blood alcohol levels by modified head space gas chromatography-mass spectrometry in drunken drivers

Amaç: Bu çalışmada trafik kazalarına karışmış sürücülerin kan alkol düzeylerinin modifiye head space gaz kromatografi-kütle spektrometresi yöntemi ile tayin edilmesi amaçlanmıştır. Metod: Ankaranın üç önemli hastanesinin acil servislerine trafik kazasına karışması sonucu getirilen sürücülerin kan örnekleri alınarak modifiye head space C/MS yöntemi ile tayin edilmiştir. Bu amaçla metanol, etanol ve izopropil alkol için head space yöntemi modifiye edilmiş, bu alkoller için tanımlanabilirlik (LOD), tayin edilebilirlik (LOQ) değerleri ile yüzde verim hesaplanmıştır. Geliştirilen analiz yöntemi 100 sürücü kan örneğine uygulanmıştır. Bulgular: Metanol, etanol ve izopropil alkol için LOD değerleri 2 mg/dl,iç Standard olarak izopropil alkol kullanıldığında metanol ve etanolun LOQ değeri 5 mg/dl olarak saptanmıştır. Bu alkollerin kandan geri alım yüzdesi ise metanol, etanol ve izopropil alkol için sırasıyla % 97,37, % 97,90 ve % 98,7 olarak belirlenmiştir. Bu metod 100 sürücü kan örneğine uygulanmış ve 39 adedinde ölçülebilir etanol saptanmıştır. On iki kan örneğinde alkol düzeyi 50 mg/dl düzeyinin üzerinde (76,55-239,27 mg/dl), 8 örnekte 25 - 50 mg/dl, 11 örnekte 10-25 mg/dl, ve 8 örnekte 10 mg/dl düzeyinin altında (6,42-9,77 mg/dl). kan alkol düzeyi ölçülmüştür. Sonuç: Türkiye'de yasal alkol limiti 50 mg/dl olarak kabul edilmiştir. Günümüzde bu alkol düzeyinin sürücülerin performanslarını olumsuz yönde etkileyebilecek derecede yüksek olarak değerlendirilmektedir. Bu nedenle biz alkol ölçümü için güvenilir ve hassas GC/MS yöntemi geliştirmiş ve 100 sürücü kan örneğine uygulamış bulunmaktayız.

Alkollü sürücülerin kan alkol düzeylerinin modifiye head space gaz kromatografi-kütle spektrometresi yöntemi ile tayini

Purpose: This study describes the analysis of ethanol blood samples from drivers involved in traffic accidents using the head space gas chromatography-mass spectrometry (GC/MS) method. Methods: We surveyed the emergency cases in three mafor hospitals in Ankara. Blood samples taken from patients who had been involved in traffic accidents were analyzed by modified head space GC/MS. With the developed method, for methanol, ethanol and isopropyl alcohol, optimization of instrument conditions, limit of detection (LOD), limit of quantitation (LOQ) and recovery of alcohols in blood samples were calculated. This method was applied to 100 drivers' blood samples. Results: LOD values for methanol, ethanol and isopropyl alcohol were 2 mg/dl, and the LOQ for methanol and ethanol was 5 mg/dl. Recovery of methanol, ethanol and isopropyl alcohol from blood samples was 97.37%, 97.90% and 98.70%, respectively. In 39 blood samples from 100 drivers, measurable alcohol results were obtained. Twelve blood samples had levels above SO mg/dl (76.55-239.27 mg/dl), eight had levels between 25 and 50 mg/dl, eleven hqd levels between 10 and 25 mg/dl, and eight had levels below 10 mg/dl (6.42-9.77.mg/dl).. Conclusion: In Turkey the. legal alcohol limit in traffic was accepted as 50 mg/dl. This limit is now considered to be high enough to influence the driver's performance negatively. Therefore, a more reliable and sensitive GC/MS method was developed. By this modified method, we studied blood samples taken from 100 drivers admitted to the emergency departments of 3 major hospitals in Ankara.

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