Meriç Arda EŞMEKAYA, Çiğdem ÖZER, Nesrin SEYHAN, Bahriye SIRAV

Radyofrekans radyasyonun sıçanların beyin dokusundaki oksidan ve antioksidan düzeylerine etkisi

Amaç: Radyofrekans radyasyonun (RF) beyin dokusu üzerinde çeşitli biyokimyasal değişikliklere neden olduğu yapılan çalışmalarda gösterilmiştir. Çalışmamızda 1800 MHz frekanslı Puls Modülasyonlu RF Radyasyonun dişi sıçanların beyin dokularında oksidatif strese neden olup olmadığını incelemeyi amaçladık. Yöntemler: Çalışmamızda dişi Wistar-Albino sıçanlar Sham-Kontrol grubu (n=10) ve RF grubu (n=10) olmak üzere iki gruba ayrıldılar. RF grubundaki sıçanlar 1 ay boyunca günde 20 dakika 1800 MHz frekanslı Puls Modülasyonlu RF Radyasyona maruz bırakıldılar. Sham-Kontrol grubuna ise herhangi bir uygulama yapılmadı. Çalışma sonunda sıçanlar ketamin (50 mg/kg) ve xylazine (50 mg/kg) anestezisi altında kalplerinden kan alınarak feda edildi. Oksidan stres lipid peroksidasyonun önemli göstergelerinden biri olan Malondialdehit (MDA), antioksidan düzey ise doğal antioksidan olan Glutatyon (GSH) miktarının ölçülmesiyle belirlendi. Total nitrik oksit (NOx) düzeyleri ise Griess yöntemi ile çalışıldı. Sonuçlar Mann-Whitney U testi ile değerlendirildi. p

Effects of radiofrequency radiation on brain tissue oxidant and antioxidant levels in rats

Objective: It has been reported that RF (Radiofrequency) Radiation induces some biochemical changes in brain tissue. In the present study we aimed to investigate whether 1800 MHz pulse modulated RF Radiation causes oxidative stress in brain tissue of the female rats. Methods: A total of 20 female Wistar-Albino rats used in the study were randomly divided into two groups: Sham- Control group (n=10) and RF group (n=10). RF rats were exposed to pulse modulated RF Radiation 20 min/day for a month. The sham-control rats were not exposed to RF Radiation. At the end of the study, rats were sacrificed by injection of ketamine (50 mg/kg) and xylazine (5 mg/kg) combination. Oxidative damage in tissues was examined by investigating the lipid peroxidation marker Malondialdhyde (MDA), antioxidant levels were assessed by measuring GSH levels. Total Nitric Oxide (NOx) levels were studied by the Griess Reaction. The results were analyzed by the Mann - Whitney U Test. The accepted level of significance was set at p

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