Işın GÜNEŞ, Ömer KURTİPEK, Faruk METİN ÇOMU, Bayazit DİKMEN, Dilek KALAYCİ, Elif BEŞKARDEŞ, Ayşe Hande ARPACİ, Mustafa ARSLAN

Geçici Hiperglisemi anında Sevofluran ve Desfluranın Eritrosit Deformabilitesi Üzerine Etkilerinin Araştırılması

Amaç: Uzun süreli hipergliseminin oluşturduğu mikro ve makrovasküler komplikasyonlar mortalite ve morbidite artışı ile birliktedir. Eritrositlerin ise yaşam süreleri boyunca uzun süre hiperglisemiye maruz kalmaları morfolojik olarak eritrositlerde deformabilitede azalma ve agregasyon gelişmesi gibi bir takım değişikliklere neden olmaktadır. Bunun sonucunda eritrositlerin yaşam sürelerinde kısalma, oksijen taşıma kapasitesinde bozukluk, doku hipoksisi gibi komplikasyonlar meydana gelmektedir. Sevofluran ve desfluranın ise eritrosit deformabilitesi üzerine etkileri ile ilgili çeşitli çalışmalar mevcuttur. Biz de bu çalışmamızda geçici olarak oluşturulan hiperglisemi sırasında sevofluran ve desfluranın eritrosit deformabilitesi üzerine etkilerini araştırmayı amaçladık. Yöntem: Çalışma Gazi Üniversitesi Deneysel ve Klinik Araştırma Merkezi'nde Gazi Üniversitesi Deney Hayvanları Etik Kurulu onayı ile yapıldı. Çalışmaya 30 rat dahil edildi. Ratlar; kontrol grubu, diyabetik-kontrol, diyabetik hiperglisemi, diyabetik–hiperglisemi-sevofluran, diyabetik-hiperglisemi- desfluran olmak üzere 5 gruba ayrıldı. Streptozosin ile diyabet oluşturulan ratlar 6 hafta yaşatıldıktan sonra geçici hipergilisemi oluşturuldu ve sevofluran ve desfluran anestezisi uygulandı. 24 saat sonra kan örnekleri alındı ve santrifüj edildi. Eritrosit deformabilitesi sabit akım filtrometre sistemleri kullanılarak ölçüldü. Rölatif rezistansının artması eritrosit deformabilitesinin azalması olarak yorumlandı. Bulgular: Diyabet oluşturulmasının kontrol grubuna göre rölatif rezistansı arttırdığı bulundu (p

Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia

Aim: Micro and macrovascular complications due to long-term hyperglycemiaare associated with increased mortality and morbidity. Erythrocytes exposedto hyperglycemia for a long time may cause morphological changes inerythrocytes such as decreased deformability and development ofaggregation. As a result, complications such as shortening life span oferythrocytes, impairment of oxygen carrying capacity, tissue hypoxia mayoccur. In our study, we would like to investigate the effects of Sevoflurane andDesflurane on erythrocyte deformability during transient hyperglycemia.Materials and Methods: In this study, 30 male Wistar albino rats were used.The animals were randomly divided into five groups, each contained 6 rats:Diabetic control (group DC), diabetic hyperglycemia group (group DH),diabetic hyperglycemia group with desflurane (group DH-D), and diabetichyperglycemia group with sevoflurane (group DH-S) groups. Another 6 ratswithout diabetes were assigned as control group (group C). Streptozotocin-induced diabetic rats were kept 6 weeks, then transient hyperglycemia wascreated, and the administration of sevoflurane and desflurane wereperformed. After 24 hours blood samples were obtained and deformabilitymeasurements were performed in erythrocyte suspensions containing Htc5% in a PBS buffer.Results: Diabetes mellitus was found to increase relative resistance in thecontrol group (p

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