Gülnur TAKE KAPLANOĞLU, Deniz ERDOĞAN, Celal ILGAZ, Mahmut BAĞIRZADE, Gülser AK, Fatma HELVACIOĞLU

Açlık ve açlık sonrası histamin ve gastrin uygulanmasında mide paryetal hücrelerinin ince yapı düzeyinde araştırılması

Amaç: Bu çalışmada açlık ile açlık sonrası histamin ve süreye koşut gastrin uygulamasının paryetal hücre yapısınaetkisinin ince yapı düzeyinde incelenmesi amaçlanmıştır. Yöntemler: Çalışmada Swiss albino cinsi erkek sıçanlardan 9 grup oluşturuldu; 1. grup: kontrol, 2. grup: 12 saataçlık, 3. grup: 12 saat açlık + 1 saat hisamin, 4. grup: 12 saat açlık + 3 dakika gastrin, 5. grup: 12 saat açlık + 3 dakikaDMSO (gastrin çözücüsü), 6. grup: 12 saat açlık + 7 dakika gastrin, 7. grup: 12 saat açlık + 7 dakika DMSO, 8. grup: 12saat açlık + 15 dakika gastrin, 9. grup: 12 saat açlık + 15 dakika DMSO. Süre bitiminde alınan doku örnekleri alışılmışelektron mikroskop takip yöntemlerinden geçirilerek, araldit CY212 kite gömüldüler. İnce kesitler Carl Zeiss EM 900elektron mikroskopta değerlendirildi. Bulgular: Yapılan elektron mikroskobik incelemede kontrol grubundan ayrıcalı olarak açlık grubunda tübülovezi-küllerin arttığı, yer yer vakuolar yapılar oluşturduğu gözlenirken, histamin uygulamasında minimal düzeyde mito-kondriyal değişiklik dışında herhangi bir farklılık saptanmamıştır. Gastrin uygulamasının ise özellikle süreye koşuthücresel dejenerasyonu arttırdığı belirlenmiştir. Bu değişiklik, özellikle çekirdek yapısı, mitokondriyonlar ve hücreiçi kanalcıklarda gözlenmiştir. Sonuç: Bu çalışmada gastrin uygulamasında gözlenen belirgin dejenerasyonun, gastrinin iki yolak üzerinden asitsalgısını arttırmasının bir sonucu olarak değerlendirilmiştir. (Gazi Med J 2011; 22: 59-64)

Ultrastructural ınvestigation of parietal cells in fasting-after fasting and the efects of histamin and gastrin

Objective: The aim of this study is to determine the ultrastructural ef ect of fasting and applications of histamineand gastrin after fasting in gastric parietal cell.Methods: In this study, nine groups were composed of Swiss albino male rats. Group 1: Control, group 2: fasting for12 hours, group 3: 12 hours fasting +1hour histamine, group 4: 12 hours fasting + 3min. gastrin, group 5: 12 hoursfasting + 3min. DMSO (solvent of gastrin), group 6: 12 hours fasting + 7min. gastrin, group 7: 12 hours fasting +7min. DMSO, group 8: 12 hours fasting + 15min. gastrin, group 9: 12 hours fasting + 15min. DMSO. At the end ofthe study time, the tissue samples were passed through routine electron microscopic preparation methods and tis-sues were embedded in araldite CY212 kit. Thin sections were evaluated on Carl Zeiss EM 900 electron microscope. Results: It was determined in the executed research, by using the electron microscope, that tubulovesicular struc-tures were increased in the fasting group independently of the control group, vacuolar structures were also ob-served in patches and histamine application did not reveal any dif erence except for mitochondrial dif erences insmall amounts. However, it was determined that gastrin application increased the cellular degeneration parallel toincreasing duration. This transformation was especially observed in nuclear structures, mitochondria and intracy-toplasmic canaliculi in the cells. Conclusion: The distinct degeneration which is observed during the gastrin application in this study was evaluated as a result of the augm entation of the acid secretion by gastrin using two ways. (Gazi Med J 2011; 22: 59-64)

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