Tüberkülozisli Sığırlarda Antioksidan Parametrelerdeki Değişimler

Bu çalışma, tüberkulozisli sığırlarda antioksidan parametrelerdeki değişimi belirlemek amacıyla yapılmıştır. Çalışmada 15 tüberkülozisli ve 15 sağlıklı inekten oluşan toplam 30 adet inek kullanılmıştır. Tüberkülozisin teşhisinde deri içi purifiye protein derivatı (PPD) mamalian tüberkülin testi kullanılmıştır. Tüm hayvanların V. jugularis'lerinden kan örnekleri alınmış ve en kısa sürede analizleri yaptırılmıştır. Gruplar arasında malondialdehid (MDA), glutatiyon peroksidaz (GSHPx), glutatiyon (GSH), vitamin E ve vitamin C bakımından istatistiksel önem saptanmasına rağmen, katalaz ve vitamin A yönünden herhangi bir önem saptanmamıştır. Sonuç olarak tüberkülozisli sığırlarda lipid peroksidasyon ve antioksidan parametrelerde önemli değişimler belirlenmiştir.

Changes in Antioxidant Parameters in Cattle with Tuberculosis

The study was conducted to detect the changes in antioxidant parameters in cattle with tuberculosis. The study was carried out on 30 cattle consisting of 15 cattle with tuberculosis and 15 healthy cattle. Intradermal purified protein derivative (PPD) mamalian tuberculin test was used for diagnosis of tuberculosis. Blood samples were taken from the jugular vein of the animals and analyzed as soon as possible. Although significant differences were determined in malondialdehide (MDA), glutathion peroxidase (GSHPx), glutatione (GSH), vitamin E and vitamin C levels between the groups, the significant changes were not determined in the catalase and vitamin A levels. In conclusion, important changes were determined in the lipid peroxidation and antioxidant parameters in the cattle with tuberculosis.

PDF

___

1. Hope JC, Villarreal-Ramos B. Bovine TB and the development of new vaccines. Comp Immunol Microbiol Infect Dis 2007; 31: 77-100.
2. Quinn PJ, Markey BK. Concise review of Veterinary Microbiology. UK: Blackwell Publishing, 2003.
3. OIE. "Bovine tuberculosis". "http://web.oie.int/eng/ normes/MMANUAL/2008/pdf/2.04.07_BOVINE_TB.pdf/ 10.02.2016.
4. Palmer MV, Waters WR. Advances in bovine tuberculosis diagnosis and pathogenesis: what policy makers need to know. Vet Microbiol 2006; 112: 181-190.
5. Cousins DV. Mycobacterium bovis infection and control in domestic livestock. Rev Sci Tech 2001; 20: 71-85.
6. Inwald J, Hinds J, Palmer S, et al. Genomic analysis of Mycobacterium tuberculosis complex strains used for the production of purified protein derivative. J Clin Microbiol 2003; 41: 3929-3932.
7. Machlin LJ, Bendich A. Free radical tissue damage: Protective role of antioxidant nutrients. FASEB J 1987; 1: 441-446.
8. Gutteridge JMC, Hallivell B. The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem Sci 1990; 15: 129-135.
9. Kohen R, Nyska A. Oxidation of biological systems: Oxidative stres phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 2002; 30: 620-650.
10. Osada H, Watanabe Y, Nishimura Y, et al. Profile of trace element concentrations in the feto-placental unit in relation to fetal growth. Acta Obstet Gynecol Scan 2002; 81: 931- 937.
11. Moore K, Roberts LC. Measurement of lipid peroxidation. Free Radic Res 1998; 28: 659-671.
12. Reddy YN, Murthy SV, Krishna DR, Prabhakar MC. Role of free radicals and antioxidants in tuberculosis patients. Indian J Tub 2004; 51: 213-218.
13. Madebo T, Lindtjorn B, Aukrust P, Berge RK. Circulating antioxidants and lipid peroxidation products in untreated tuberculosis patients in Ethiopia. Am J Clin Nutr 2003; 78: 117-122.
14. Akiibinu MO, Ogunyemi EO, Shoyebo EO. Levels of oxidative metabolites, antioxidants and neopterin in Nigerian pulmonary tuberculosis patients. Europ J Gen Med 2011; 8: 213-218.
15. Placer AZ, Linda LC, Johnson B. Estamination of product of lipid peroxidation (Malonlydialdehyde) in biochemical systems. Anal Biochem 1966; 16: 359-364.
16. Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 1976; 71: 952-958.
17. Sedlak J, Lindsay RHC. Estimation of total protein bound and nonprotein sulfhydryl groups in tissue with Ellmann's reagent. Anal Biochem 1968; 25: 192-205.
18. Goth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 1991; 196: 143-152.
19. Kyaw A. A simple colorimetric method for ascorbic acid determination in blood plasma. Clin Chim Acta 1978; 16: 151-157.
20. Ewer K, Deeks J, Alvarez L, et al. Comparison of T-cellbased assay with tuberculin skin test for diagnosis of Mycobacterium tuberculosis infection in a school tuberculosis outbreak. Lancet 2003; 361: 1168-1173.
21. Wood PR, Rothel JS. In vitro immunodiagnostic assays for bovine tuberculosis Vet Microbiol 1994; 40: 125-135.
22. Ulrichs T, Munk ME, Mollenkopf H, et al. Differential T cell responses to Mycobacterium tuberculosis ESAT6 in tuberculosis patients and healthy donors. Eur J Immunol 1998; 28: 3949-3958.
23. De La Rua-Domenec R, Goodchild AT, Vordermeier HM, et al. Ante mortem diagnosis of tuberculosis in cattle: A review of the tuberculin tests, gamma interferon assay and other ancillary diagnostic techniques. Res Vet Sci 2006; 81: 190-210.
24. Waters WR, Palmer MV, Pesch BA, et al. Lymphocyte subset proliferative responses of Mycobacterium bovis infected cattle to purified protein derivative. Vet Immunol Immunopathol 2000; 77: 257-273.
25. Gutteridge JMC. Free radicals in disease processes: A compilation of cause and consequence. Free Radic Res Commun 1993; 19: 141-158.
26. Kızıl O, Özdemir H, Karahan M, Kızıl M. Oxidative stres and alterations of antioxidant status in goats naturally infected with Mycoplasma agalactia. Rev Med Vet 2007; 158: 326-330.
27. Castillo C, Hernandez J, Lopez-Alonso M, Miranda M, Benedito JL. Values of plasma lipid hydroperoxides and total antioxidant status in healthy dairy cows: Preliminary observations. Arch Anim Breed 2003; 46: 227-233.
28. Moore K, Roberts LC. Measurement of lipid peroxidation. Free Radic Res 1998; 28: 659-671.
29. Jack CI, Jackson MJ, Hind CR. Circulating markers of free radical activity in patients with pulmonary tuberculosis. Tuber Lung Dis 1994; 75: 132-137.
30. Grimble RF. Malnutrition and the immune response. Impact of nutrients on cytokine biology in infection. Trans R Soc Trop Med Hyg 1994; 88: 615-619.
31. Nathan CF, Brukner LH, Silverstein SC, Cohn ZA. Extracellular cytolysis by activated macrophages and granulocytes. Pharmacologic triggering of effector cells and the release of hydrogen peroxide. J Exp Med 1979; 149: 84-99.
32. YN Reddy, SV Murthy, DR Krishna, MC Prabhakar. Role of free radicals and antioxidants in tuberculosis patients. Indin J Tuberc 2004; 51: 213-218.
33. Gutteridge JMC, Hallivell B. The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem Sci 1990; 15: 129-135.
34. Leung HW, Vang MJ, Mavis RD. The cooperative interaction between vitamin E and vitamin C in suppression of peroxidation of membrane phospholipids. Biochim Biophys Acta 1981; 664: 266-272.
35. Vannucchi H, Jordoa-Junior AA, Iglessias AC, Morandi MV, Chiarello PG. Effects of different diatery concentrations of vitamin E on lipid peroxidation in rats. Arch Latinoam Nutr 1997; 47: 34-37.
36. Doba T, Burton GW, Ingold KU. Antioxidant and coantioxidant activity of vitamin C. The effect of vitamin C, either alone or in the presence of vitamin E or a water soluble vitamin E analogue, upon the peroxidation of aqueous multilamellar phospholipids liposomes. Biochim Biophys Acta 1985; 835: 298-303.
37. Niki E, Saito T, Kawakami A, Kamiya Y. Inhibition of oxidation of methyl linoleate in solution by vitamin E and vitamin C. J Biol Chem 1984; 259: 4177-4182.
38. Madanat A, Zendulkova D, Pospisil Z. Contagious agalactia af sheep and goats: A review. Acta Vet 2001; 70: 403-412