Füsun TEMMAOĞULLARI, Ali HAYAT, Rahşan YILMAZ, Zafer ÖZYILDIZ

The effects of Rosemary (Rosemarinus officinalis) extract on wound healing in rabbits

Bu araştırmada, %5’lik Rosemarinus officinalis (biberiye) ekstraktının yara iyileşmesi üzerine etkileri iyot çözeltisi ve serum fizyolojik ile karşılaştırılmalı olarak günlük gözlem, histopatolojik ve istatistiksel yöntemlerle incelendi. Çalışmada 6 erkek, 6 dişi Yeni Zellanda tavşanı kullanıldı. Her tavşanın sırt bölgesine deneysel olarak 3.14 cm çapında üçer adet, tam katlı yara oluşturuldu. Bu yaralardan birine günlük olarak %5’lik biberiye ekstraktı, diğerine %10’luk povidone-iyot solüsyonu ve sonuncusuna ise kontrol amaçlı %0.9’luk serum fizyolojik içeren gazlı bez tamponu uygulandı. Tüm hayvanların yara çapları 4, 7, 10 ve 14. günlerde kumpasla ölçüldü ve her defasında saat yönünde ilerleyerek farklı bölgelerden biyopsi örnekleri alındı. Yara iyileşmesi, histopatolojik olarak nötrofil ve makrofaj infiltrasyonları ile fibrosit, fibroblast proliferasyonu, kollagen birikimi, epitelizasyon ve damarlaşma yönünden değerlendirildi. Bu çalışmanın amacı, biberiye ekstraktının yara iyileşmesi üzerindeki etkisinin iyot ve serum fizyolojik solüsyonları ile karşılaştırmalı olarak incelenmesidir.

Tavşanlarda biberiye (Rosemarinus officinalis) ekstraktının yara iyileşmesi üzerine etkileri

In the present study, we examined the effects of 5% Rosemarinus officinalis (rosemary) extract on wound healing as compared to those of povidone-iodine and isotonic saline solution by daily visual observation, histopathological and statistical methods. Six male and six female New Zealand rabbits were used in the study. On dorsal aspect of each rabbits, experimentally 3.14 cm in diameter, three full-thickness wounds was constituted. One of these wounds received daily 5% rosemary extract, another one received 10% povidone-iodine solution and the last one received 0.9% isotonic saline solution containing the gauze tampon for control purposes. Size of each wound was measured on days 4, 7, 10 and 14 using a caliper and biopsies were taken from different regions in clockwise direction proceedingly. Wound healings were assessed histopathologically in terms of neutrophil and macrophage infiltration with fibrocyte and fibroblast proliferation, collagen deposition, epithelialization, and vascularization. The aim of this study was to investigate the effect of rosemary extract on wound healing compared to povidone-iodine and isotonic saline solution.

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