Meme Tümörlü Köpeklerde Serum 17β-estradiol Kolesterol ve Trigliserid Düzeylerinin Klinik Önemi

Çalışmada, Ankara ve çevresinden Ankara Üniversitesi Veteriner Fakültesi ve Serbest Veteriner kliniklerine getirilen ve klinik ve histopatolojik muayene sonunda meme tümörü tanısı konmuş ve klinik olarak sağlıklı görünen dişi köpeklere ait (n=15/grup) kan serumlarında 17β- estradiol, toplam kolesterol ve trigliserid değerleri incelenmiştir. Kan örnekleri köpeklerin ön ayak venalarından alınmış, 4000 RPM’de 10 dakika santrifüj edilerek serumları elde edilmiştir. Serum 17β- estradiol analizleri kompetitif kemiluminesan enzim immünoassay (c-CLEIA), trigliserid analizleri bikromatik, toplam kolesterol analizleri ise polikromatik son nokta teknikleri kullanılarak otoanalizörde gerçekleştirilmiştir. Meme tümörlü köpeklerde serum ortalama değerleri 17β- estradiol için 142,70±16,00 pg/ml; toplam kolesterol için 250,10±17,60 mg/dl; trigliserid için 106,20±7,50 mg/dl olarak hesaplanmıştır. Klinik olarak sağlıklı görünen köpeklerde ise, serum 17β- estradiol düzeyi 59,30±6,50 pg/ml; toplam kolesterol düzeyi 204,90±13,20 mg/dl; trigliserid düzeyi 77,00±4,50 mg/dl olarak bulunmuştur. Mann- Whitney U Testi ile kontrol ve deneme grupları karşılaştırılmış ve meme tümörlü köpeklerde parametrelerin kontrol grubuna oranla önemli düzeyde yüksek olduğu saptanmıştır (p

Clinical Importance of Serum 17β-estradiol Total Cholesterol and Triglyceride Levels in Dogs with Mammary Gland Tumors

In the study, serum samples from 15 healthy dogs and 15 dogs with mammary tumors from Ankara and surrounding Faculty and Veterinary clinics were analyzed. From these samples the serum levels of 17β-estradiol, total cholesterol and triglyceride were determined to investigate the relationship between these parameters and canine mammary tumors. Blood samples taken from the left front foot vena of each dog were centrifuged with 4000 revolutions for 10 minutes. The analysis of 17β-estradiol was carried out based on competitive chemilumminescent enzyme immunoassay (c-CLEIA), triglyceride on bichromatic end point technique and total cholesterol on polychromatic end point technique and performed by an autoanalyser. In tumorous dogs, the mean serum level of 17β-estradiol was found 142,70±16,00 pg/ml, the mean level of total cholesterol was 250,10±17,60 mg/dl and the mean level of triglyceride was 106,20±7,50 mg/dl. In healthy dogs, the mean levels for the same parameters in the same order were 59,30±6,50 pg/ml, 204,90±13,20 mg/dl and 77,00±4,50 mg/dl. Control and experimental groups were compared statistically by Mann-Whitney U Test and levels of all parameters mentioned above were found higher for the dogs with mammary tumors than healthy dogs (p

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