L-lizin ve L-ornitinin manda karaciğer ve böbrek doku arginazi üzerine etkisi

Bu çalışmada L- ornitin ile L- lizinin manda karaciğer ve böbrek doku arginazını farklı konsantrasyonlarda inhibe ettiği ortaya konulmuştur. L- ornitinin 20 mM konsantrasyonunda karaciğer doku arginazını %60, böbrek doku arginazını ise %39’ını inhibe ederken 80 mM’lık konsantrasyonda ise karaciğer doku arginazını %89, böbrek doku arginazını %86 oranında inhibe ettiği görülmüştür. Diğer taraftan, 20 mM’lık L- lizin karaciğer doku arginazını %47 inhibe ederken böbrek doku arginazını %41 oranında inhibisyona uğratmıştır. 80 mM’lık L-lizin denendiğinde karaciğer doku arginazını %81, böbrek doku arginazını %79 inhibisyona uğrattığı saptanmıştır. Farklı substrat konsantrasyonlarında karaciğer dokusu 20 mM L- arginin konsantrasyonundan sonra doyuma ulaşırken böbrek dokusu 50 mM’dan sonra doyuma erişmiştir. Karaciğer 2,4 mM, böbrekte ise 8 mM olarak bulunmuştur. Bu sonuçlara göre 50 mM L- ornitin ve L- lizin ilavesiyle farklı substrat konsantrasyonlarında manda karaciğer ve böbrek dokusunda arginaz Km’lerinin de farklı olduğu bulunmuştur. Sonuç olarak; L- ornitin ve L- lizinin, manda karaciğer ve böbrek doku arginazının farklı oranlarda inhibisyonuna neden olduğu görülmüştür. L- ornitin ve L- lizin amino asitleri manda karaciğer ve böbrek doku arginazını karışık tipte (kompetatif- nonkompetatif) inhibe ettiği saptanmıştır.

Effect of L-lysine and L-ornithine on buffalo liver and kidney tissue arginase

In this study, at different concentrations, L- ornithine and L- lysine were demonstrated to be inhibited arginase activity levels in buffalo liver and kidney tissue. At 20 mM concentration, Lornithine inhibited liver tissue arginase and kidney tissue arginase by 60 and 39% respectively. Whereas at 80 mM inhibitory effect of L- ornithine were found 89 % and 86 % in liver and kidney tissue arginases, respectively. On the other hand, L- lysine at 20 mM concentration was determined to inhibit liver and kidney tissue arginase activity levels by 47 % and 41 % , respectively. When effect of higher concentration (80 mM) of L- lysine was examined, it was determined that inhibition of liver and kidney tissue arginases were 81 % and 79 %, respectively. With different substrates, liver tissue was saturated after 20 mM L- arginine whereas kidney tissue was saturated after 50 mM of L- arginine. Based on these results , when 50 mM L- ornithine and Llysine were added at different substrate concentrations, Km of buffalo arginase was determined to be different at liver ( 2.4 mM) and kidney (8 mM) tissues. In conclusion, arginase activity levels in liver and kidney tissue were determined to be inhibited differently. Arginase activity in Buffalo liver and kidney tissue arginases were demonstrated to be inhibited via mixed inhibition (competetive- non competetive) by L- ornithine and L- lysine.

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