Armağan Erdem ÜTÜK, Abdurrauf YÜCE, Sami ŞİMŞEK

Determination of serum malondialdehyde levels in sheep naturally infected with Dicrocoelium dendriticum

Dicrocoeliuin Dendriticum ile Doğal Enfekte Koyunlarda Serum Malondialdehid Seviyesinin Belirlenmesi Bu çalışmanın amacı, Dicrocoeliuin dendriticum ile doğal enfekte koyunlarda serum malondialdehid seviyesindeki değişikliklerin araştırılmasıdır. Serum malondialdehid aktivitesi D.dendriticum ile doğal enfekte 20 koyunun serumunda ölçülmüştür. Bu koyunlar, kesim sonrasında safra kanalları ile safra kesesinin muayenesine göre seçilmiş, negatif kontrol olarak da D.dendriticum'un görülmediği sağlıklı 17 kuzu serumu kullanılmıştır. Neticede, enfekte ve kontrol grubu koyunlarda serum malondialdehid seviyeleri arasındaki fark istatistiksel olarak önemli bulunmuş (P

Dicrocoelium dendriticum ile doğal enfekte koyunlarda serum malondialdehit seviyesinin belirlenmesi

The aim of this study was to investigate the changes of serum malondialdehyde levels in sheep naturally infected with Dicrocoeliiim dendriticum. Scrum malondialdehyde activity was measured in 20 sheep that were naturally infected with D.dendriticum. These sheep were selected to examination of bile ducts and gall bladder after slaughtering. Scores were obtained for the positives and 17 D.dendriticum negative healthy controls. The difference between malondialdehyde levels of sheep infected with D.dendriticum and control group was statistically significant (P

PDF

___

1. Theodoridis Y, Duncan JL, MacLean JM, Himonas CA. Pathophysiological studies on Dicrocoelium dendriticum in sheep. Vet Parasitol 1991;36:61-66.
2. Soulsby EJL. Helminths, Arthropods and Protozoa of Domesticated Animals. 6th Edition, Bailliere and Tindall: London, 1968.
3. Jithendran KP, Baht TK. Prevalence of dicrocoeliosis in sheep and goats in Himachal Pradesh, India. Vet Parasitol 1996; 61: 265-271.
4. Terry DW. Dicrocoelium dendriticum: the life cycle in Britain. J Helminthol 1969; 43: 403-416.
5. Steele JH. Parasitic zoonoses. In:, Hillyer GV, Hopla CE. (Editors). Handbook Series in Zoonoses, Volume 3. CRC, Boca Raton, Florida, 1982: 33-52.
6. Sanchez-Andrade R, Paz-Silva A, Suarez JL, Arias M, Lopez C, Morrondo P, Scala A. Serum antibodies to Dicrocoelium dendriticum in sheep from Sardinia (Italy). Prev Vet Med 2003; 57: 1-5.
7. Ferre I, Ortega-Mora LM, Rojo-Vazguez FA. Prevalance of Dicrocoelium dendriticum infection in Leon province (NW Spain). Prev Vet Med 1994; 21: 147-154.
8. Ambrosi M. La diagnostica coprologica nelle elmintiasi di allevamento caso delle distomatosi dei ruminanti. Praxis Vet 1991; 12: 17-21.
9. Rojo-Vazquez FA, Cordero del Campillo M, Diez N, Chanton-Schaffer M. Relationship existing between the number of eggs in the feces and parasitic charge during ovine natural Dicrocoelium dendriticum infestation. Revue Med Vet 1981; 132:601-607.
10. Romero FJ, Bosch-Morell F, Romero MJ, Jareno EJ, Romero B, Marin N, Roma J. Lipid peroxidation products and antioxidants in human disease. Environ Health Perspect 1998; 106: 1229-1234.
11. Gal tier P, Vandenberghe Y, Coecke S, Eeckhoutte C, Larrieu G, Vercruysse A, Differential inhibition of rat hepatic glutation S-transferase isoenzymes in the course of fascioliasis. Mol Biochem Parasitol 1991;44:255-260.
12. Kılıç E, Yazar S, Saraymen R, Yalçın Ş. Changes in the level of serum malondialdehyde in patients infected with Giardia intestinalis. Acta Parasitologica Turcica 2003; 27: 184-186.
13. Kılıç E, Yazar S, Saraymen R, Özbilge H. Serum lipid peroxidation level in patients with Taeniasis saginata. Acta Parasitologica Turcica 2004; 28: 91-93.
14. Kılıç E, Yazar S, Saraymen R. Lipid peroxidation level in patients with Blastocystosis. J. Inonu Univ. Med. Fac. 2003; 10: 1-3.
15. Kargın F, Fidancı UR. Kidney diseases and antioxidative metabolism in dogs. Tr J Vet Anim Sci 2001; 25: 607-613.
16. Placer ZA, Cushmann L, Johnson BC. Estimation of products of lipid peroxidation in biochemical systems. Anal Biochem 1966; 16: 359-364.
17. Wolff K, Hauser B, Wild P. Dicrocoeliosis in sheep: pathogenesis and liver regeneration after therapy. Berl Munch Tierarzt Woch 1984; 97: 378-387.
18. Manga-Gonzalez MY, Gonzalez-Lanza C, Del-Pozo P. Dicrocoelium dendriticum (Trematoda, Digenea) eggs in the faeces of lambs and ewes in Porma Basin (Leon, NW Spain). Ann Parasitol Hum Comp 1991; 66: 57-61.
19. Kılıç E, Yazar S, Saraymen R, Özbilge H. Serum malondialdehyde level in patients infected with Ascaris lumbricoides. World J Gastroenterol 2003; 9: 2332-2334.
20. Panazzo PM, Basso D, Balint L, Biasin MR, Bonvicini P, Metus P. Altered lipid peroxidation/glutathione ratio in experimental extrahepatic cholestasis. Clin Exp Pharmacol Physiol 1995;22:266-271
21. Sanchez-Compos S, Tunon MJ, Gonzalez P, Gonzalez-Gallego J. Oxidative stress and changes in liver antioxidant enzymes induced by experimental dicroceliosis in hamsters. Parasitol Res 1999; 85: 468-474.
22. Galtier P, Larrieu G, Tufenkji AE, Franc M. Incidence of experimental fascioliasis on the activity of drug-metabolizing enzymes in lamb liver. Drug Metab Dispos 1986; 14: 863-872.
23. Galtier P, Eeckhoutte C, Larrieu G. Fasciola hepatica: liver enzymes in rats and interaction with chemical inducers. Exp Parasitol 1987; 63: 189-194.
24. Chaudhary AK, Nokubo M, Reddy GR, Yeola SN, Morrow JD, Blair IA, Marnett LJ. Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver. Science 1994; 265: 1580-1582.
25. Draper H, Hadley M. A review of recent studies on the metabolism of exogenous and endogenous malondialdehyde. Xenobiotica 1990; 20: 901-907.