OKSİDATİF STRESE MARUZ BIRAKILMIŞ RATLARA VERİLEN BİOCHANİN A‘NIN SERUMDAKİ BAZI BİYOKİMYASAL PARAMETRELER ÜZERİNE ETKİSİ

İzoflavonlar birçok bitkide bulunan fitokimyasallardır. Biochanin A 5,7 – dihidroksi 4’- metoksi izoflavon-dur. İzoflavonlar antioksidan özellik gösterir. Bu araştırmada, H2O2 ileoksidatif strese maruz bırakılan sıçanlar üzerine gavaj yöntemiyle verilen Biochanin A’nın koruyucu etkileri araştırıldı. Çalışmada MDA, enzimler (katalaz, glutatyon peroksidaz), GSH düzeyleri ölçüldü. Bu amaçla Biochanin A, H2O2, Kontrol ve H2O2+ Biochanin A grupları kullanıldı. Deneylerden elde edilen sonuçlara göre, H2O2 MDA düzeyleri kontrol grubuna göre arttı (p<0.0001). Biocha-nin A’nın etkisiyle H2O2+ Biochanin A grubu MDA düzeyi H2O2 grubuna göre azaldı (p<0.001). Tüm grupların katalaz ve glutatyon peroksidaz enzim aktiviteleri kontrol grubuna göre artmıştır (p<0.0001). Sonuçlarımız, Biochanin A antioksidan özellikte bir madde olduğunu göstermektedir.

Anahtar Kelimeler:

H2O2, MDA, Katalaz, Glutatyon Peroksidaz, GSH, Biochanin A, Serum, Sıçan

PDF

___

1. Machlin, L. J., Bendich, A., Maestro, R. F., 1980, An Approach to Free Radicals in Medicine and Biology, Acta Physiol. Scand. Suppl., 492, 153-168.
2. Fabes J., 1987, Free Radical Tissue Damage, Protective Role of Antioxidant Nutrients, 1, 441-445.
3. Yu, P.B., 1994, Cellular Defenses Against Damage From Reactive Oxygen Species, Physiol. Rev., 74, 1.
4. Dergel, R., 1992, Lipid Peroxsitation a Common Pathogenetic Mechanism, Exp. Toxicol Pathol., 44 (4), 169-181.
5. Hollmann, P.C.H., Batan, M.B., 1997, Absorption, metabolism and healht effect of dietary flavonoids in man, Biomed. Pharmacotherapy, 51 , 305-310.
6. Formica, J.V., Regelson, W., 1995, Review of the biology of quercetin and related bioflavo-noids, Food Chem. Toxicol 33: 1061-1080.
7. Hanasaki, Y., Owaga, S., Fukui, S., 1994, The correelation betwen active oksigen scavenging and antioxidative effects of flavonoids, Free Radic Biol Med; 16: 845-50.
8. Takahama, U., 1985, Inhibition of lipoxygenase-dependent peroxitation by quercetin: mecha-nisms of antioxidative function, Phytochemistry; 24: 1443-6.
9. Majid, S., Khanduja, K.L., Gandhi, R.K., Kapur, S., Sharma, R.R., 1991, Influence of ellagik asit on antioxidant defense system andlipid perok-sitation in mice, Biochem Pharmacol; 42: 1441–5.
10. a. Harper, A., Kerr, D.J., Gescher, A., Chipman, J.K., 1999.Antioxidant effects of isoflavonoids and lignans, and protection against DNA oxida-tion. Free Radical Res. 31, 149–160. b. Ruiz-Larrea, M.B., Mohan, A.R., Paganga, G., Miller, N.J.,Bowell, G.P., Rice-Evance, C., 1999. Anti-oxidant activity of phytoestrogenic isoflavones. Free Radical Res. 26, 63–70.
11. Placer, Z.A., Cushman, L.L., Johnson, B.C., 1966, Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems, Anal Biochem; 16: 359–364.
12. Goth, L., 1991, A simple method for determi-nation of serum catalase activity and revision of reference range, Clin Chim Acta; 196: 143-152.
13. Lawrence, R.A., Burk, R.R., 1976, Glutathione peroxidase activity in selenium-deficient rat liver, Bioch Bioph Res Commun., 71(4): 952-958.
14. Lowry, O.O., Rosebrough, N.J., Farr, A.L., Ran-dall, R.I., 1951, Protein measurement with folin phenol reagent, J Biol Chem, 193: 265-275.
15. Sedlak, J., Lindsay, R.H., 1968, Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent, Anal. Biochem., 25:192-205
16. a.Wei, H., Bowen, R., Cai, Q., Barnes, S., Wang, Y., 1995. Antioxidant and antipromoti-onal effects of the soybean isoflavone genistein. Proc. Soc. Exp. Biol. Med. 208, 124–130. b. Cai, Q., Wei, H., 1996. Effect of dietary genistein on antioxidant enzyme activities in SENCAR mice. Nutr. Cancer 25,1–7.
17. Rodrigo, R., Rivera, G., Orellana, M., Araya, J., Bosco, C., 2002, Rat kidney antioxidant respon-se to long-term exposure to flavonol rich red wine, Life Sci., 71:2881-289.
18. Molina, M.F., Sanchez-Reus, I., Iglesias, I., Be-nedi, J., 2003, Quercetin, a flavonoid antioxi-dant, prevent and protects against ethanol-induced oxidative stres in mouse liver, Biol Pharm Bull, 26:1398-140.
19. Appelt, L.C., Reicks, M.M., 1999. Soy induces phase II enzymes but does not inhibit dimethyl-benz[a]anthracene-induced carcinogenesis in fe-male rats. J. Nutr. 129, 1820–1826.