Rafet TOK, AYHAN UYSAL, OKTAY BURMA, İbrahim Hanifi ÖZERCAN

Mekanik distansiyon uygulanan safen vende apopitozis üzerine vazodilatatör solüsyonların etkisi

Amaç: Bu çalışmamızda, safen vende mekanik distansiyonun sebep olduğu endotelyum harabiyetinde, apopitotik aktivitenin rolünü araştırmayı amaçladık. Gereç ve Yöntem: Çalışmaya her iki cinsten, koroner arter bypass greft operasyonuna alınan, venöz yetmezliği olmayan 32 hasta dahil edildi. Herbir grupta 8 hasta olmak üzere hastalar 4 gruba ayrıldı; Grup1 (Kontrol), Grup 2 (Papaverin), Grup 3 (Verapamil) ve Grup 4 (Nitrogliserin). Herbir grubun VSM'larından doku örnekleri alındı. Doku kesitlerinde immünohistokimyasal boyama ile proapopitotik bax, kaspaz-9 ve antiapopitotik bcl-2 boyanma yüzdeleri araştırıldı. Bulgular: Gruplarda yer alan safen venlerde proapopitotik Bax (+) hücre yüzdeleri sırasıyla 85.00 ± 6.89, 64.63 ± 8.73, 23.75 ± 4.20 ve 22.00 ± 7.69 olarak saptandı. Verapamil ve nitrogliserin grupları arasında istatistiksel bir fark bulunmazken, bu gruplardaki değerler kontrol ve papaverin grubuna göre anlamlı derecede düşük bulundu (p

The effects of vasodilator solutions on apoptosis in saphenous vein with mechanical distention application

Objective: This study explores the influence of vasodilators on the apoptotic activity related to endothelial damage caused by mechanical distension of the saphenous vein. Materials and Methods: Thirty-two patients without varicose veins undergoing coronary artery bypass graft operations were divided into four equal groups. Group I (control) Normal Saline, group II Papaverine group III Verapamil and group IV Nitroglycerine solutions. Percentange of immunohistochemical reactivity, pro-apoptotic bax and kaspaz-9 and anti-apoptotic bcl-2 were measured in the samples. Results: The percentage of cells which were positive for Bax (+) In Group I (Control) was 85.00 ± 6.89, Group II (Papaverine) 64.63 ± 8.73, Group III (Verapamil) 23.75 ± 4.20 and Group IV (Nitroglycerine) 22.00 ± 7.69. While there was no statistical difference between verepamil and nitroglycerin groups, they both had significantly decreased percentage of cells with proapoptotic Bax (+) compared to the control and the papaverine groups (p<0.001). Percentage of cells that were stained with the antiapoptotic BCL (+) stain were lowest in the control (2.50 ± 1.41) and the Papaverine (2.75 ± 1.28) groups and significantly higher in the Verapamil (7.25 ± 1.83) and Nitroglycerine (8.88 ± 2.30) groups (p<0.001). There was no difference between the groups with respect to Caspase-9 immunoreactivity. Conclusion: The results of this study suggest that addition of Verapamil and or Nitroglycerine to the solutions that are used in the preparation of the saphenous vein might have a beneficial effect on the reduction of the apoptotic activity.

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