Ramin GHASEMİ SHAYAN, Nasrin HOSSEİNZAD MANİE

The Effect of Troxerutin Consumption on the Symptoms of Morphine Withdrawal Syndrome in Male Mice

Introduction: Chronic morphine use is associated with increased oxidative stress and inflammatory factors. Troxerutin is a natural bioflavonoid and has antioxidant and anti-inflammatory effects that could relieve morphine withdrawal syndrome. Aim: The effects of troxerutin on morphine dependence in mice Method: Troxerutin was prepared in three different doses (50, 100, and 200 mg/kg) via a normal saline solution. After examining the composition of troxerutin, the experiment was performed in five groups of 8 mice. One group received eight days of increasing doses (10, 20, 30, 40, 50, and 60 mg/kg) of morphine SC (subcutaneously) with normal saline (10 ml/kg) IP (Intraperitoneal), and one group received only normal saline and the other three groups received three different doses of troxerutin solved in normal saline as the carrier of troxerutin along with morphine. On the ninth day, withdrawal symptoms were recorded after naloxone injection and blood samples were examined for antioxidant factors. Results: The total withdrawal score in the 50 mg/kg dose of the troxerutin group with morphine was significantly lower than the morphine-saline group (p<0.001***). The total withdrawal score in the 100 mg/kg dose of troxerutin group with morphine was significantly lower than the morphine-saline group (p<0.01**). Antioxidant tests showed a significant increase in the level of TAC of serum (p<0.001***) and a decrease in the level of MDA of serum (p<0.001***) in all doses of troxerutin. (50, 100 and 200 mg/kg) As performing the locomotion activity, no significant motility dysfunction or paralysis was observed in mice after using troxerutin. (All P>0.05). Conclusion: Troxerutin reduces the symptoms of morphine withdrawal syndrome in a dose-dependent manner. The results of antioxidant tests declared that troxerutin would increase the level of TAC (Total Antioxidant Capacity) and decrease the level of MDA (Malondialdehyde) in the serum of mice possibly due to its antioxidant properties.

Keywords:

: morphine, troxerutin, withdrawal syndrome, dependence, MDA TAC, mice,

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