Umay HAKGÜDER, Ünsal Veli ÜSTÜNDAĞ, Burçin Alev TÜZÜNER, Nevin GENÇ, Hazal İPEKÇİ, Reyhan ÖZÇELİK, Tuğba TUNALI-AKBAY, Göksel ŞENER, Ayşen YARAT, Ebru EMEKLİ ALTURFAN

Doğal Yaşlanma Modelinde Egzersiz ve Kalori Kısıtlamasının Beyin ve Testiste Etkileri

Amacımız doğal yaşlanma modelinde egzersiz ve kalori kısıtlama-sının sıçanlarda beyin ve testiste çeşitli doku hasarı ve antioksidan parametreler üzerine etkilerinin incelenmesidir. Bu amaçla Spragu-e-Dawley sıçanlar kontrol (K), yaşlı (Y), kalori kısıtlaması uygulanan yaşlı (YKK), egzersiz uygulanan yaşlı (YE) ve kalori kısıtlaması ile egzersiz uygulanan yaşlı (YKKE) olmak üzere 5 gruba ayrıldı. Kont-rol grubu üç aylık genç hayvanlardan oluşturuldu. Diğer gruplar 15 aylık sıçanlardan oluşturuldu. Egzersiz ve kalori kısıtlaması 6 hafta boyunca uygulandı. Deney sonunda beyin ve testis dokuları homojenatlarında lipit peroksidasyon (LPO), nitrik oksit (NO), glu-tatyon (GSH) düzeyleri ile süperoksit dismutaz (SOD), katalaz (CAT), glutatyon-S-transferaz (GST) ve doku faktörü (DF) aktiviteleri tayin edildi. Çalışma sonucunda K grubu ile kıyaslandığında Y grubunda, beyin ve testis LPO, NO düzeyleri ve DF aktivitelerinin arttığı, GSH düzeyleri ile SOD, CAT ve GST aktivitelerinin ise azaldığı gözlendi. Doğal yaşlanma modelinde beyin ve testiste DF aktivitesi ile be-raber oksidan hasarda artış gözlenmiş olup, özellikle yaşlanmada egzersiz ve kalori kısıtlamasının beyinde antioksidan düzeyleri üze-rinde olumlu etkileri belirlenmiştir.

Anahtar Kelimeler:

Yaşlanma, beyin, testis, oksidan, antioksidan

Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model

The aim of our study was to examine the effects of exercise and cal-orie restriction on various tissue damage and antioxidant parame-ters in the brain and testis of rats in a natural aging model. For this purpose, male Sprague-Dawley rats were the control group (C), the elderly (A), the elderly with calorie restriction (ACR), the elderly who were exercised (AE) and the elderly who were exercised with calorie restriction (ACRE), they were divided into 5 groups. The con-trol group was composed of three-month-old animals. The other group consisted of 15-month-old rats. Exercise and calorie restric-tion were applied for 6 weeks. At the end of the experiment, lipid peroxidation (LPO), nitric oxide (NO), glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), glutathione-S-trans-ferase (GST) and tissue factor (TF) were determined in brain and testicular tissues homogenates. As a result of the study, the A group’s brain and testis LPO, NO levels and TF activity increased, GSH levels and SOD, CAT and GST activities decreased, when com-pared to the C group. As a result of our study, an increase in oxidant damage was observed with TF activity in the brain and testis in the natural aging model, and positive effects of exercise and calorie restriction on the antioxidant levels in the brain were determined, especially in aging.

Keywords:

aging, brain, testis, oxidant, antioxidant,

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