Cihan COŞKUN, Hümeyra ÖZTÜRK EMRE, Alper GÜMÜŞ, Sami Uzun, Serhat KARADAĞ, Ahmet BEHLÜL, Muhammet Emin DÜZ, Macit KOLDAŞ, Savaş ÖZTÜRK
Diyabetik ve Diyabetik Olmayan Kronik Böbrek Yetmezliğinde Dinamik Tiyol Disülfit Homeostazı ve İleri Protein Oksidasyon Ürünleri (AOPPs)
Amaç: Kronik böbrek yetmezliği (KBY) ve tip 2 diyabet (DM)’in etyolojisinde oksidatif stresin önemlirolü bulunmaktadır. Tiyol grubu içeren bileşikler indirgeyici özellikleri ile oksidatif strese karşı savunmadaönemli bir görev üstlenirler. Dinamik tiyol-disülfit homeostazının bozulması KBY ve DM dahilpek çok hastalığın gelişiminde rol oynar. Protein moleküllerinin oksidasyonu sonucunda oluşan ilerioksidasyon protein ürünleri (AOPPs)’de biyolojik olarak aktif olup hem böbrek hasarı hem de diyabetgelişimde önemli bir rol oynarlar.Gereç ve Yöntem: Çalışmaya 47 (28 erkek, 19 kadın) KBY, 27 (13 erkek, 14 kadın) KBY+DM hastasındanoluşan hasta grupları ile 30 kişi (16 erkek, 14 kadın)’den oluşan kontrol grubu dahil edildi. Totalile serbest tiyol düzeyleri “Modifiye Ellman Metotu” ile tespit edildi. AOPPs düzeyleri ise, Witko-Sarsat’ıngeliştirmiş olduğu metot ile çalışıldı.Bulgular: Total tiyol, serbest tiyol ve AOPPs düzeyleri bakımından hasta grupları ile kontrol grubuarasında fark bulundu (her biri için, p<0.001). KBY ve KBY+DM gruplarındaki AOPPs düzeyleri ile GFRdeğerleri arasında negatif ilişki (sırasıyla, r=-0.585, ve p<0.001, r=-0.479) var iken, AOPPs ile hsCRPdüzeyleri arasında pozitif ilişki bulundu (sırasıyla, r=0.416, p<0.001 ve r=0.474, p<0.001).Sonuç: KBY hastalarında DM’nin varlığı tiyol ve AOPPs sonuçlarını etkilemedi. Tiyol düzeyleri KBYhastalarındaki oksidan durum ile ilişkili iken, KBY hastalığının ilerlemesi ile ilişkili bulunmadı. AOPPsdüzeyleri ise, KBY hastalığındaki oksidatif stresin yanı sıra, KBY hastalığının ilerlemesi ile de ilişkilibulundu.
Anahtar Kelimeler:
Tiyol-disülfit homeostazı, ileri oksidasyon protein ürünleri, oksidatif stres, kronik böbrek yetmezliği
Dynamic Thiol Disulphide Homeostasis and Advanced Oxidation Protein Products (AOPPs) in Diabetic And Non-Diabetic Chronic Renal Failure Disease
Objective: Oxidative stress has important role in the etiology of chronic renal failure (CRF) and type2 diabetes (DM). The compounds containing thiol groups with their reducing properties undertake animportant task on the defense against oxidative stress. Disruption of dynamic thiol-disulfide homeostasisplays a role in the development of many diseases including CRF and DM. Advanced oxidationprotein products (AOPPs) resulting from the oxidation of protein molecules are biologically active andplay an important role in both kidney damage and DM development.Material and Methods: Patient groups consist of 47 (28 male, 19 female) CRF and 27 (13 male, 14female) CRF+DM subjects and control group consists of 30 subjects (16 male, 14 female) were includedin the study. Total and native thiol levels were determined by “Modifiye Ellman Method”. As for AOPPslevels were studied by the method developed by Witko-Sarsat.Results: It was found between patient groups and control group in terms of levels of total thiol, freethiol and AOPPs (p<0.001 for each group). While there was negative correlation between AOPPs levelsand GFR values in CRF and CRF+DM groups (r=-0.585, p<0.001 and r=-0.479, p<0.001, respectively),it was found positive correlation between AOPPs and hsCRP levels (r=0.416, p<0.001 and r=0.474,p<0.001, respectively).Conclusion: The presence of DM in CRF patients did not affect the results of thiols and AOPPs.While thiol level was associated with oxidant status in patients with CRF, it was not found associatedwith progression of CRF disease. As for AOPPs level was also found associated progression of CRF aswell as oxidative stress in the CRF disease.
Keywords:
Thiol disulphide homeostasis advanced oxidation protein products, oxidative stress, chronic renal failure, type 2 diabetes,
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- Yayın Aralığı: Yılda 3 Sayı
- Başlangıç: 2011
- Yayıncı: İstanbul Üniversitesi
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