Özgür ÇELEBİ, Fatih BÜYÜK, Mitat ŞAHİN, Doğan AKÇA, Keti SİDAMONİDZE, Eka ZHGENTİ

The mismatched isolation of Brucella strains from nomic hosts

Amaç: Bu çalışmada Kafkas Üniversitesine ait Brucella suş koleksiyonunun bir bölümü çeşitli yöntemler ile değerlendirilmiştir. Gereç ve Yöntem: Suşların orjini abort hikayesi olan sığır ve koyunlar oluşturudu. Otuz adet Brucella suşu (16'sı koyun ve 14'ü sığır orjinli) rastgele seçildi ve bu çalışmaya dahil edildi. Standardize fenotipik karakterizasyona ilaveten suşların moleküler tiplendirmesi için cins spesifik Real Time PCR ve tür spesifik Bruce-Ladder PCR uygulandı. Bulgular: Onaltı koyun örneğinin 15'i B. melitensis ve 14 sığır örneğinin 13'ü B. abortus olarak identifiye edildi. İlginçtir ki, koyun aborte fötusten izole edilen bir suş B. abortus ve sığırdan izole edilen bir suş B. melitensis olarak tanımlandı. Bununla birlikte, koyun fötusundan elde edilen bir suş bütün fenotipik özellikleri yönünden Brucella cinsine benzer karakterde olmasına rağmen genotipik olarak (ne Real Time PCR ne de Bruce-Ladder PCR) Brucella spp. olarak doğrulanamadı ve ileride karakterize edilmek üzere Ochrobactrum spp. şüpheli olarak saklandı

Primer konaklardan izole edilen uyumsuz Brucella türleri

Aim: A small part of field Brucella strain collection of Kafkas University was evaluated by different methods in this study. Materials and Methods: The strains were originated from cattle and sheep which had a history of abortion. Thirty field Brucella strains (16 were originated from sheep and 14 were from cattle) were randomly selected and included in the study. In addition of standardized phenotypical characterization, genus specific real-time PCR and species specific Bruce-Ladder PCR were conducted for molecular typing. Results: Out of 16 sheep and 14 cattle samples 15 and 13 were identified as B. melitensis and B. abortus, respectively. Interestingly one strain was characterized as B. abortus isolated from aborted sheep foetus and the other as B. melitensis from aborted cattle milk sample. Furthermore one strain from aborted sheep foetus was unable to be confirmed as Brucella spp. by genotypically (neither by real-time PCR nor Bruce-Ladder PCR) though all phenotypic features were similar to Brucella genus and kept under strict conditions for future characterization on suspicion of Ochrobactrum spp.

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