Mastitisli sığır sütlerinden izole edilen Enterococcus faecalis izolatlarının plazmid profillerinin incelenmesi

Öztürk M, Türkyılmaz S. Mastitisli sığır sütlerinden izole edilen Enterococcus faecalis izolatlarının plazmid profillerinin incelenmesi. Amaç: Bu çalışmada mastitisli sığır sütlerinden izole dilen Enterococcus faecalis izolatlarının plazmid profillerinin incelenmesi amaçlandı. Gereç ve Yöntem: Çalışmanın materyalini geçmiş yıllarda izole edilmiş olan 54 E. faecalis izolatı oluşturdu. Bakterilerden plazmid izolasyonunda alkali liziz yöntemi modifiye edilerek kullanıldı. Bulgular: Plazmid izolasyonu sonucunda, izolatların 19'unun bir ile sekiz arasında plazmid taşıdığı, plazmid boyutlarının da yaklaşık olarak 0.6 kb ile 41 kb arasında değiştiği tespit edildi. Çalışmada, 5 izolat tek plazmid ve 14 izolatın ise birden fazla plazmid taşıdığı saptandı. Plazmid izolasyonunu takiben, profil içerikleri kontrol edilerek, plazmidler arasında kümeleme analizi yapıldı. Benzer plazmid profilinde olan iki veya daha fazla suş içeren 6 küme belirlendi Öneri: Bundan sonraki çalışmalarda, mastitisli sığır sütlerindenizole edilen diğer enterokok türlerinin plazmitleriaraştırılıp, bu plazmitlerin antibiyotik dirençliliğine katkılarıincelenebilir.

Investigation of plasmid profiles of Enterococcus faecalis isolated from mastitic cow milks

turk M, Turkyilmaz S. Investigation of plasmid profiles of Enterococcus faecalis isolated from mastitic cow milks.Aim: The purpose of this study was to investigate the plasmid profiles of Enterococcus faecalis isolated from cow milk samples with mastitis.Materials and Methods: As a material, 54 E. faecalis strains isolated in the past years from milk samples with mastitis were used. Modified alkaline lysis method was used for plasmid isolation from bacteria. Results: Results revealed that 19 of isolates have plasmids varying one to eight and lengths of those were ranged approximately between 0.6 kb and 41 kb. It was determined that five isolates had only one plasmid while the other 14 had more plasmids. Following the plasmid isolation, cluster analysis was performed between the same and similar plasmids after their profile contends checked. Six clusters that containing two or more strains with similar plasmid profile were formed. Conclusions: Further studies should be designed to investigate the plasmids of other enterococci stains isolated from mastitis cow’s milks whether plasmids are responsible for antibiotic resistance

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