Devrim Sarıpınar AKSU, Yavuz Selim SAĞLAM, Taylan AKSU

The investigation of neuroprotective effects of pomegranate juice against low level lead induced oxidative stress in rats brain

Amaç: Bu çalışmada, düşük düzeyde kurşun (Pb) maruziyetinin sıçanlarda beyin dokusu ve antioksidan sistem üzerinde oluşturduğu hasara karşı nar suyunun (NS) nöroprotektif potansiyelinin araştırılması amaçlandı. Gereç ve Yöntem: Araştırmada kullanılan toplam 40 adet rat, her birinde 10'ar rat bulunan 4 gruba ayrıldı. Kontrol grubu (1), standart rat yemi ve içme suyuyla beslendi. Pozitif kontrol grubuna içme suyuyla günlük 2000 ppm kurşun (kurşun asetat); düşük tedavi grubuna (3), içme suyuyla günlük 2000 ppm kurşun ve gavajla 30 µL NS; yüksek tedavi grubuna (4) ise içme suyuyla günlük 2000 ppm kurşun ve gavajla 60 µL NS verildi. Araştırma 5 hafta sürdürüldü. Sıçan beyinlerinde malondialdehide (MDA) ve glutatyon (GSH) seviyeleri ile süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri belirlendi. Ayrıca beyin dokusunda histopatolojik incelemeler yapıldı. Bulgular: Nar suyu kurşunun sebep olduğu lipid peroksidasyonu azaltarak (düşük MDA düzeyi) antioksidan enzim (SOD ve CAT) aktivitelerini ve GSH düzeyini artırarak oksidatif stresi hafifletti. Sadece kurşun alan grupta şiddetli nörodejeneratif değişiklikler görüldü. Nar suyunun, kurşunun beyin dokusunda oluşturduğu hücresel hasarı kısmen önlediği belirlendi.Öneri: Düzenli nar suyu tüketimi artan yoğun sanayileşmeden kaynaklanan kronik kurşun maruziyetine karşı faydalı olabilir.

Düşük seviyede kurşunun ratların beyin dokusunda oluşturduğu oksidatif strese karşı nar suyunun nöroprotektif etkilerinin araştırılması.

Aim: The aim of the study was to investigate the neuroprotective potential of pomegranate juice (PJ) against the damage of brain tissue and antioxidant system induced low level lead (Pb) exposure in rats. Materials and Methods: A total of 40 rats were divided into four groups containing 10 rats in each. The control group (1) was fed standart rat feed and daily water. A positive control group (2) received a daily dose of 2000 ppm lead (lead acetate) in drinking water; a low treatment group (3) that received a daily dose of 2000 ppm lead together with 30 µL PJ by oral gavage; and a high treatment group (4) that received 2000 ppm lead and 60 µL PJ by oral gavage daily. The experiment was lasted for 5 weeks. Levels of malondialdehyde (MDA) and glutathione (GSH) were determined as well as the activities of superoxide dismutase (SOD) and catalase (CAT). Morever, histopathological examination was also performed in the brain of the rats. Results: Pomagranete juice alleviated oxidative stress by decreasing lipid peroxidation ( low MDA level) and increasing the activities of antioxidant enzymes (SOD and CAT) and GSH level in the rats exposed to lead. Severe neurodejenerative changes were observed in only groups received lead. Cellular damage of the brain was partially prevented by PJ. Conclusion: Regular consumption of pomegranate juice may provide significant benefits against the threat of chronic heavy metal exposure due to increasing intensive industrialization.

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