Tuncer KUTLU, Enver BEYTUT, Uğur AYDIN, Serpil DAĞ, Emin KARAKURT, Özgür AKSOY, Hilmi NUHOĞLU, Ayfer YILDIZ, İsa ÖZAYDIN

Sığırlarınoküleryassıhücrelikarsinomlarındaapoptozis

Amaç: Hücre ölümü özellikle de apoptozis hücre konusunda en çok çalışılan konular arasında yer almaktadır. Hastalıklardaki apoptozis mekanizmasının anlaşılması sadece hastalıkların patogenezinin anlaşılmasına değil hastalıkların tedavisine de yardımcı olacaktır. Bu çalışmada sığırlarda gözlenen oküler yassı hücreli karsinomların (Bovine ocular squamouse cell carcinoma, BOSCC) derecelendirilmesi ve farklı anaplastik özellikler gösteren bu tümörlerde apoptozisin değerlendirilmesi hedeflendi. Gereç ve Yöntem: BOSCC’lerde apoptozisin incelenmesi amacıyla, formalinde tespit edilmiş 10 adet kötü differensiye, 10 adet orta differensiye ve 10 iyi diferensiye olan toplam 30 adet tümör dokusu kullanıldı. Histopatolojik inceleme için hematoksilen & eozin (H&E); apoptozisin tespit için tüm BOSCC’lerde TUNEL (Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) boyaması yapıldı. Bulgular: BOSCC’lerde malignite artıkça apoptozisin azaldığı saptandı (p

Apoptosisinbovineocularsquamousecellcarcinomas

Aim: Cell death, especially apoptosis, is one of the most studied topics regarding cells. Understanding the mechanism of apoptosis in diseases will help understand the pathogenesis of diseases, as well as the treatment. This study aims to grade the Bovine ocular squamous cell carcinoma (BOSCC) and to evaluate apoptosis in tumors with different anaplastic features. Materials and Methods: A total of 30 formalin-fixed tumor tissues consisting of 10 poorly-differentiated, 10 moderately-differentiated, and 10 well-differentiated tissues were used to examine apoptosis in BOSCCs. Hematoxylin & eosin (HE) was performed for histopathological examination and TUNEL staining was performed for detection of apoptosis in all BOSCCs. Results: It was determined that apoptosis decreased as the malignancy increased in BOSCCs (p

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