Attenüe Parapoxvirus ovis’in koyunlarda bazı serum sitokin ve antikor seviyelerine etkisi

Amaç: Parapoxvirus ovis (ORFV) enfeksiyonu küçük ruminantların önemli bulaşıcı viral hastalıklarından birisidir. Bu araştırma ile attenüe orf virus aşı suşu uygulanan koyunların kan serumlarında nötralizan antikor varlığı ile sitokin miktarlarının belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada ORFV’ye karşı antikora sahip olmayan 6-8 aylık 10 adet Akkaraman erkek toklu kullanıldı. Tüm hayvanlara attenüe ORFV’nin 0,5 ml’si ile skarifikasyon işlemi yapıldı. Aşılamadan önce 0. saat (kontrol) ve sonraki 6., 12., 24., 48., 72., 96., 120. saat, 7., 14., 21., 30., 60., 90., 120., 150. ve 180. günlerde vena jugularis’ten serum örnekleri alındı. Elde edilen serumlarda nötralizan antikor varlığı araştırıldı. Buna ilave olarak toplanan serum örneklerinde proinflamatuar özelliğe sahip (Th1) sitokinlerden IL-1β, IL-12, IFN-γ, TNF-α, anti inflamatuar özelliğe sahip (Th2) sitokinlerden IL-4, ve IL-10 düzeyleri ELISA yöntemi kullanılarak belirlendi. Bulgular: IL-1β, IL-12, IFN γ, TNF-α ve IL-10 seviyelerinde kontrol serumlarına göre istatistiki açıdan önemli bir fark meydana gelmediği (p>0,05) görülmekle beraber, IL-4 düzeyinde 30. günde istatistiki açıdan bir artış tespit edildi (p

Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep

Aim: Parapoxvirus ovis (ORFV) infection is one of important contagious viral diseases of small ruminants. The aim of this study was to determine the concentration of cytokine and neutralizing antibody in blood serum of sheep that were carried out attenuated orf virus vaccine strain. Materials and Methods: In this study, 10 male Akkaraman sheep which are 6-8 months of age and tested negative for antibodies against ORFV were used. Scarification was applied with 0.5 ml of attenuated ORFV to each animal. Serum samples were obtained from vena jugularis at 0. hour (control) before the inoculation and at the following 6., 12., 24., 48., 72., 96., 120. hours, 7., 14., 21., 30., 60., 90., 120., 150. and 180. days. Neutralizing antibody presence was investigated in obtained serums. In addition, levels of IL-1β, IL-12, IFN-γ, TNF-α from proinflammatory cytokines (Th1) and IL-4 and IL-10 from antiinflammatory cytokines (Th2) were determined by ELISA. Results: ORFV caused fluctiations but no statistically significant change in levels of IL-1β, IL-12, IFNγ, TNF-α and IL-10 were detected compared to control sera (p>0,05), and significant increase in IL-4 level was detected in the 30th day (p

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