Ümit GÜRBÜZ, Hatice Ahu KAHRAMAN, Banu KANDİL, Emrah SUR, İlknur TEKDEMİR

Kuru Dinlendirme İşleminin Akkaraman Irkı Erkek Kuzuların Longissimus Thoracis ve Longissimus Lumborum Kaslarının Sarkomer Yapısı Üzerine Etkisi

Amaç: Araştırmada, Akkaraman ırkı erkek kuzuların longissimus thoracis (LT) ve longissimus lumborum (LL) kaslarından elde edilen etlere, 14 gün süre ile kuru dinlendirme yöntemi uygulanarak dinlendirme periyodu boyunca sarkomer uzunluklarında meydana gelen değişikliklerin belirlenmesi hedeflenmiştir. Gereç ve Yöntem: Materyal olarak, 11 aylık 6 adet Akkaraman ırkı erkek kuzunun longissimus thoracis ve longissimus lumborum kaslarından elde edilen etler kullanılmıştır. Kuru dinlendirme uygulaması için etler, +1°C'de, % 85 rutubet ve 0,2-0,5m/sn hava akımı olan soğutma dolabında 14 gün boyunca dinlenmeye bırakılmıştır. Dinlendirme işleminin, başlangıç (0), 7 ve 14. günlerinde alınan örnekler, rutin histolojik takip işlemlerinden sonra Crossmon'ın üçlü boyama yöntemiyle boyanıp sarkomer uzunlukları ölçülmüştür. Preparatlar ışık mikroskobu (Leica DM 250) ile değerlendirilmiş ve gerekli bölgelerin görüntüleri kamera ile dijital ortamda kaydedilmiştir. Bulgular: Kuru dinlendirme işlemine tabi tutulan LT ve LL kas gruplarının, dinlendirme periyodu boyunca sarkomer uzunluklarının 1,71- 1,87µm arasında değişim gösterdiği belirlenmiştir. LT kaslarının sarkomer uzunluklarının 7. güne kadar artış gösterip, sonrasında sabit kaldığı, LL kas grubundaki artışın ise 14. güne kadar sürdüğü belirlenmiştir. Aynı zamanda, 14 günlük süre zarfında LL kaslarında ölçülen ortalama sarkomer uzunluklarının LT kaslarına kıyasla daha uzun olduğu tespit edilmiştir. Dinlendirme periyodu boyunca ölçülen ortalama sarkomer uzunluklarındaki değişimler ile LT ve LL kas grupları arasındaki farklılıkların istatistiksel açıdan önemli olduğu görülmüştür (p

Effect of Dry Aging Application on Sarkomeric Structure of M. Longissimus Thoracis and M. Longissimus Lumborum in Akkaraman Male Lambs

Aim: In the study, it was aimed to determine the changes in sarcomere lengths of M. longissimus thoracis (LT) and M. longissimus lumborum (LL) obtained from Akkaraman breed male lamb.Materials and Methods: The LT and LL muscles obtained from Akkaraman breed male lambs of 11-month old were used. For dry aging, meats were aged for 14 days at +1 °C with 0.2-0.5 m/s airflow and 85% humidity. Samples were taken at the beginning (0), 7th and 14th days of the aging process, stained with Crossmon's triple staining method after routine histological procedures. Cross-sections were evaluated under a light microscope and sarcomere lengths were measured. Images were recorded digitally. Results: It was determined that mean sarcomere lengths of LT and LL muscle groups ranged from 1,71 to 1,87 μm during the aging period. It was detected that the sarcomere lengths of LT muscles was increased up to 7th day, following it remained constant. The sarcomere lengths of LL muscle increased until the 14th day. It was also found that the mean of sarcomere lengths of LL muscles were longer than LT muscles during the aging period. There were significant differences among the muscle groups (p

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