Rosa KEBBİ, Omar BESSEBOUA, Mohamed NAİT MOULOUD, Abdelhanine AYAD

Köpeklerde Rhipicephalus’a (sp) karşı Peganum harmala ve Glaucium flavum alkaloidinin in vitro akarisit aktivitesi

Amaç: Kene kontrolü için amaçlanan temel ilaç sorunu konakçılar üzerinde uygulama dozuna gelişen dirençtir. Glaucium flavum ve Peganum harmala'dan alkaloitlerin akarisit etkileri doğal ortamda enfekte olan köpeklerden toplanan invitro olarak yetişkin dişi Rhipicephalus sp türünde değerlendirildi. Gereç ve Yöntem: Her iki ekstraktın akarisit faaliyetleri (3.12, 6.2, 12.5, 25 ve 50 mg / ml), erişkin daldırma testi, üreme indeksi ve inhibisyon yumurtlama kullanılarak değerlendirildi. Bulgular: Metanolik ekstraktların neden olduğu mortalite yüzdesi her iki bitkinin % 3.66 ila 50 mg / ml arasındaki konsantrasyonlarda test edildiğinde % 41.66 ila 75 arasında değişmekteydi ve kontrole kıyasla önemli ölçüde farklıydı (p

In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog

Aim: The major problem of drug intended for tick control is resistancedeveloped to application doses on the hosts. The acaricidal effectsof alkaloids from Glaucium flavum and Peganum harmala wereevaluated in vitro on adult female of Rhipicephalus sp. collected innaturally infected dogs.Materials and Methods: The acaricide activities of the both extracts(3.12, 6.2, 12.5, 25 and 50 mg/ml) were evaluated using theadult immersion test, reproductive index and inhibition oviposition.DMSO (1%) and SEB (1μl/ml) were used as a negative and positivecontrol.Results: The percent mortality caused by the methanolic extractsof both plants varied from 41.66 to 75 %, when tested at concentrationsranging from 3.12 to 50 mg/ml, and significantly differentcompared to control (p

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Abduz-Zahir A, Abdul-Rahuman A, 2012. Evaluation of different extracts and synthesised silver nanoparticles from leaves of Euphorbia prostrata against Haemaphysalis bispinosa and Hippobosca maculate. Vet Parasitol. 187(3-4), 511-520.
Abdel-Ghany HS, Fahmy MM, Abuowarda MM, Abdel-Shafy S, et al., 2019. In vitro acaricidal effect of Melia azedarach and Artemisia herba-alba extracts on Hyalomma dromedarii (Acari: Ixodidae): embryonated eggs and engorged nymphs. J Parasit Dis. 43(4), 696-710.
Ait Abderrahim L, Taïbi K, Ait Abderrahim C, 2019. Assessment of the antimicrobial and antioxidant activities of Ziziphus lotus and Peganum harmala. Iran J Sci Technol Trans A: Sci. 43(2), 409-414.
Arafa AM, Mohamed MES, Eldahmy SI, 2016. The aerial parts of yellow horn poppy (Glaucium flavum Cr.) growing in Egypt: isoquinoline alkaloids and biological activities. J Pharm Sci Rese. 8(5), 323.
Avinash B, Supraja N, Prasad TNVKV, Priya CS, 2017. Evaluation of acaricidal activity of Azadirachta indica extracts against Rhipicephalus (Boophilus) microplus and its GC-MS analysis. Int J Sci. 6, 980-992.
Azizi M, Sedaghat S, Tahvildari K, Derakhshi P, Ghaemi A, 2017. Synthesis of silver nanoparticles using Peganum harmala extract as a green route. Green Chem Lett Rev. 10(4), 420-427.
Bensalem S, Soubhye J, Aldib L, Bournine L, et al., 2014. Inhibition of myeloperoxidase activity by the alkaloids of Peganum harmala L. (Zygophyllaceae). J Ethnopharmacol. 154(2), 361-369.
Boulaaba M, Kalai FZ, Dakhlaoui S, Ezzine Y, et al., 2019. Antioxidant, antiproliferative and anti-inflammatory effects of Glaucium flavum fractions enriched in phenolic compounds. Med Chem Res. 28(11), 1995-2001.
Bournine L, Bensalem S, Peixoto P, Gonzalez A, et al., 2014. Revealing the anti-tumoral effect of Algerian Glaucium flavum roots against human cancer cells. Phytomedicine. 20(13), 1211-1218.
Castilho CV, Fantatto RR, Gaínza YA, Bizzo HR, et al., 2017. In vitro activity of the essential oil from Hesperozygis myrtoides on Rhipicephalus (Boophilus) microplus and Haemonchus contortus. Rev Bras Farmacogn.27(1), 70-76.
Daemon E, Maturano R, Monteiro CMO, Goldner MS, et al., 2012. Acaricidal activity of hydroethanolic formulations of thymol against Rhipicephalus sanguineus (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Vet Parasitol. 186(3-4), 542-545.
De Oliveira PR, Bechara GH, Morales MAM, Mathias MIC, 2009. Action of the chemical agent fipronil on the reproductive process of semi-engorged females of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari:Ixodidae). Ultrastructural evaluation of ovary cells. Food Chem Toxicol. 47(6), 1255-1264.
Divya TM, Soorya VC, Amithamol KK, JulietS, Ravindran R, et al., 2014. Acaricidal activity of alkaloid fractions of Leucas indicaSpreng against Rhipicephalus (Boophilus) annulatus tick. Trop Biomed. 31(1), 46-53.
Drummond RO, Ernest SE, Trevino JL, Gladney WJ, et al., 1997. Boophilus annulatus and Boophilus microplus: laboratory tests for insecticides. J Econ Entomo. 66(1), 130-133.
Engstrom MT, Karonen M, Ahern JR, Baert N, et al., 2016. Chemical structures of plant hydrolyzable tannins reveal their in vitro activity against egg hatching and motility of Haemonchus contortus nematodes. J Agric Food Chem. 64(4), 840-851.
Friesen KJ, Kaufman WR,2000. Cypermethrin inhibits egg development in the ixodid tick, Amblyomma hebraeum. Pestic. Biochem. Physiol. 76(1), 25-35.
Frostholm A, Evans JE, Cummings SL, Rotter A, 2000. Harmaline- induced changes in gamma aminobutyric acid receptor A subunit mRNA expression in murine olivocerebellar nuclei. Brain Res Mol Brain Res.85(1-2), 200-208.
Godara R, Katoch R, Yadav A, Ahanger RR, et al., 2015. In vitro acaricidal activity of ethanolic and aqueous floral extracts of Calendula officinalis against synthetic pyrethroid resistant Rhipicephalus (Boophilus) microplus. Exp Appl Acarol. 67(1), 147-157.
Gomes GA, Monteiro CMO, Julião LDS, Maturano R, et al., 2014. Acaricidal activity of essential oil from Lippia sidoides on unengorged larvae and nymphs of Rhipicephalus sanguineus (Acari: Ixodidae) and Amblyomm cajennense (Acari: Ixodidae). Exp Parasitol. 137, 41-45.
Goncalves K, Toigo E, Ascoli B, Von Poser G, et al., 2007. Effects of solvents and surfactant agents on the female and larvae of cattle tick Boophilus microplus. Parasitol Res. 100(6), 1267-1270.
Gray j, Dantas-Torres F, Estrada-Peña A, Levin M, 2013 Systematics and ecology of the brown dog tick, Rhipicephalus sanguineus.Ticks Tick Borne Dis. 4(3), 171-180.
Kebbi R, Nait-Mouloud M, Hassissen L, Ayad A, 2019. Seasonal activity of ticks infesting domestic dogs in Bejaja Province, Northern Algeria. Onderstepoort J Vet Res. 86(1).
Klafke GM, De Albuquerque TA, Miller RJ, Schumaker TTS, 2010. Selection of an ivermectin-resistant strain of Rhipicephalus microplus (Acari: Ixodidae) in Brazil. Vet Parasitol. 68, 97-104.
Komeili G, Hashemi M, Bameri-Niafar M, 2016. Evaluation of antidiabetic and antihyperlipidemic effects of Peganum harmala seeds in diabetic rats. Cholesterol.
Landau SY, Provenza FD, Gardner DR, Pfister JA, Knoppel EL, Peterson C, et al., 2009. Neem-tree (Azadirachta indica Juss.) extract as a feed additive against the American dog tick (Dermacentor variabilis) in sheep (Ovisaries). Vet Parasitol. 165(3-4), 311-317.
Lumaret JP, Errouissi F, Floate K, Römbke J, et al., 2012. A Review on the Toxicity and Non-Target Effects of Macrocyclic Lactones in Terrestrial and Aquatic Environments. Curr Pharm Biotechnol. 13(6), 1004-1060.
Matallah F, Benakhla A, Bouattour A, 2013. Infestation du chien par Rhipicephalus sanguineus dans deux régions de l’extrême nord-est de l’Algérie. Rev Elev Med Vet Pays Trop. 66(3), 97-101.
Miller RJ, Almazan C, Ortiz-Estrada M, Davey RB, George JE, De Leon AP, 2013. First report of fipronil resistance in Rhipicephalus (Boophilus) microplus of Mexico. Vet Parasitol. 191, 97–101.
Misra P, Khaliq T, Dixit A, Sen Gupta S, et al., 2008. Antileishmanial activity mediated by apoptosis and structure-based target study of peganine hydrochloride dihydrate: an approach for rational drug design. J Antimicrob Chemother. 62(5), 998-1002.
Monteiro CM, Maturano R, Daemon E, Catunda-Junior FEA, et al., 2012. Acaricidal activity of eugenol on Rhipicephalus microplus (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Parasitol Res. 111(3), 1295–1300.
Muhammad S, Fatima A, Yahaya MM, 2012. The phytochemical components of Leucas martinicensis that cause repellence of adult mosquito. Int J Mod Botany. 2(1), 1-5.
Olivo CJ, Heimerdinger A, Ziech MF, Agnolin CA, et al., 2009. Rope tobacco aqueous extract on the control of cattle ticks. Cienc Rural. 39(4), 1131-1135.
Perez-Cogollo LC, Rodriguez-Vivas RI, Ramirez-Cruz GT, Miller RJ, 2010. First report of the cattle tick Rhipicephalus microplus resistant to ivermectin in Mexico. Vet Parasitol. 168, 165- 169.
Petropoulos SA, Karkanis A, Martins N, Ferreira ICFR, 2018. Halophytic herbs of the Mediterranean basin: An alternative approach to health. Food Chem Toxicol. 114,155-169.
Ravindran R, Juliet S, Sunil AR, Ajith Kumar KG, et al., 2011. Eclosion blocking effect of extract of Leucas aspera (Lamiiaceae) on Rhipicephalus (Boophilus) annulatus. Vet Parasitol. 179(1-3), 287-290.
Sodaeizadeh H, Rafieiolhossaini M, Van Damme P, 2010. Herbicidal activity of a medicinal plant, Peganum harmala L., and decomposition dynamics of its phytotoxins in the soil. Ind Crops Prod. 31(2), 385-394.
Sariosseiri A, Moshaverinia A, Khodaparast MHH, Kalidari GA, 2018. In vitro acaricidal effect of Melia azedarach ripe fruit extract against Dermanyssus gallinae (Acari: Dermanyssidae). Persian J Acarol. 7(2), 203-208.
Shang X, Guo X, Li B, Pan H, Zhang J, Zhang Y, Miao X, 2016. Microwave-assisted extraction of three bioactive alkaloids from Peganum harmala L. and their acaricidal activity against Psoroptes cuniculi in vitro. J Ethnopharmacol. 192, 350-361.
Suau R, Cabezudo B, Rico R, Nájera F, et al., 2004.Photochemical variations within populations of Platycapnossaxicola Wilk. Biochem Syst Ecol. 6, 565-572.
Valduga AT, Gonçalves IL, Magri E, Finzer JRD, 2018. Chemistry, pharmacology and new trends in traditional functional and medicinal beverages. Food Res Int. 120(5), 478- 503.
Valente PP, Amorim JM, Castilho RO, Leite RC, et al., 2013. In vitro acaricidal efficacy of plant extracts from Brazilian flora and isolated substances against Rhipicephalus microplus(Acari: Ixodidae). Parasitol Res. 113(1), 417-423.
Walker JB, Keirans JE, Horak IG, 2000. The Genus Rhipicephalus. Cambridge, Cambridge University Press.
Walker AR, Bouattour A, Camicas JL, Estrada-Peña A. et al., 2014. Ticks of domestic animals in Africa: A guide to identification of species, Bioscience Reports, Edinburgh.3, 210.