Rüstem DUMAN, Sibel YAVRU, Atilla ŞİMŞEK, Oya BULUT, Oğuzhan AVCI

Keçilerde Caprine Arthritis Encephalitis Virus enfeksiyonu

Amaç: Bu çalışma Konya'da özel işletmelerde yetiştirilen keçilerde caprine arthritis encephalitis virus (CAEV) enfeksiyonunun serolojik varlığının araştırılması, ırk ve cinsiyete göre farklılığının incelenmesi amacı ile yapıldı.Gereç ve Yöntem: Araştırmada (Konya-Merkez, Cihanbeyli, Taşkent, Güneysınır ve Karapınar) 162 Ankara keçisi ve 491 kıl keçisi olmak üzere toplam 653 adet keçi kan serum örneği CAEV'ye karşı oluşan antikor varlığı yönünden ticari olarak temin edilen competitive-inhibition ELISA ile test edildi.Bulgular: Örneklerden 8 (%7.14) Ankara ve 58 (%15.72) kıl keçisinde seropozitiflik belirlendi. Örnekleme yapılan ilçelerde enfeksiyonun görülme oranı değerlendirildiğinde istatistiki fark tespit edilmezken (P>0.05), ırk ve cinsiyet arasında fark belirlendi (P

Caprine Arthritis Encephalitis Virus infection in goats

Aim: The aim of this study was to serologic investigation of caprine arthritis encephalitis virus (CAEV) infection in goats in private goat farms of Konya region, and occurrence of infection in breed and sex differences were also evaluated.Materials and Methods: In the research, total 653 goats' blood serums (Central Konya, Cihanbeyli, Taşkent, Güneysınır, and Karapınar) obtained from 162 Angora goat and 491 hair goats were tested for antibodies against to CAEV by commercially available competitive-inhibition ELISA.Results: Eight (7.14%) Angora and 58 (15.72%) hair goats were detected as seropositive. There was no statistically significance (P>0.05) determined in the infection occurrence in the distinct, while statistically significance was determined (P

PDF

___

Acar A, Yavru S, Kale M, Bulut O, Avci O, Pehlivanoglu F, 2008. Caprine arthritis encephalitis virus infection in kids with arthritis. Indian Vet J, 85, 824-825.
Azkur AK, Gazyagci S, Aslan ME, 2011. Serological and epi- demiological investigation of bluetongue, maedi-visna and caprine arthritis-encephalitis viruses in Kirikkale district in Turkey. Kafkas Üniv Vet Fak Derg, 17, 803-808.
Barquero N, Arjona A, Domenech A, Toural C, de las Heras A, Fernandez-Garayzabal JF, Ruiz-Santa Quiteria JA, Go- mez-Lucia E, 2011. Diagnostic performance of PCR and ELISA on blood and milk samples and serological survey for small ruminant lentiviruses in central Spain. Vet Rec, 168, 20.
Blacklaws BA, Berriatua E, Torsteinsdottir S, Watt NJ, de And- res D, Klein D, Harkiss GD, 2004. Transmission of small ru- minant lentiviruses. Vet Microbiol, 101, 199-208.
Blacklaws BA, 2012. Small ruminant lentiviruses: Immuno- pathogenesis of visna-maedi and caprine arthritis and en- cephalitis virus. Comp Immunol Microbiol Infect Dis, 35, 259-269.
Brinkhof JMA, Moll LD, Van Maanen C, Houwers DJ, 2010. Use of serology and polymerase chain reaction for the rapid eradication of small ruminant lentivirus infections from a sheep flock: A case report. Res Vet Sci, 88, 41-43.
Burgu I, Akca Y, Ozkul A, Karaoglu T, Cabalar M, 1994. An- tibody prevalence of caprine arthritis encephalitis virus (CAEV) in goats in Turkey. Deutsche Tierarztliche Woc- henschrift, 101, 390-391.
Cutlip RC, Lehmkuhl HD, Sacks JM, Weaver AL, 1992. Preva- lence of antibody to caprine arthritis-encephalitis virus in goats in the United States. J Am Vet Med Assoc, 200, 802-805.
Dawson M, Biront P, Houwers DJ, 1982. Comparison of sero- logical tests used in three state veterinary laboratories to identify maedi-visna virus infection. Vet Rec, 111, 432-434.
de Andres D, Klein D, Watt NJ, Berriatua E, Torsteinsdottir S, Blacklaws BA, Harkiss GD, 2005. Diagnostic tests for small ruminant lentiviruses. Vet Microbiol, 107, 49-62.
Devilbiss B, Neelis D, Ochoa J, Ziegler J, Barrington G, Allen A, 2013. Computed tomography findings in a 5-year-old Australian Cashmere goat (Capra hircus) suffering leuko- encephalomyelitis due to caprine arthritis encephalitis vi- rus. Can Vet J, 54, 960-964.
Elfahal AM, Hussien MO, Enan KA, Taha KM, Salih DA, Halfawi RH, Mohammed ZA, El-Hussein AM, 2013. Investigation of caprine arthritis-encephalitis virus in Sudan using com- petitive enzyme-linked immunosorbent assay. Veterinary World, 6, 558-562.
Eltahir YM, Dovas CI, Papanastassopoulou M, Koumbati M, Giadinis N, Verghese Nikolakaki S, Koptopoulos G, 2006. Development of a semi-nested PCR using degenerate pri- mers for the generic detection of small ruminant lentiviru- ses proviral DNA. J Virol, 135, 240-246.
Gjerset B, Jonassen CM, Rimstad E, 2007. Natural transmis- sion and comparative analysis of small ruminant lentivi- ruses in the Norwegian sheep and goat populations. Virus Res, 125, 153-161.
Herrmann LM, Cheevers WP, McGuire TC, Adams DS, Hutton MM, Gavin WG, Knowles DP, 2003. Competitive-inhibiti- on enzyme-linked immunosorbent assay for detection of serum antibodies to caprine arthritis-encephalitis virus: diagnostic tool for successful eradication. Clin Diagn Lab Immunol, 10, 267-271.
Konishi M, Nagura Y, Takei N, Fujita M, Hayashi K, Tsukiok M, Yamamoto T, Kameyama K, Sentsui H, Murakami K, 2011. Combined eradication strategy for CAE in a dairy goat farm in Japan. Small Rumin Res, 99, 65-71.
Lago N, Lopez C, Panadero R, Cienfuegos S, Pato J, Prieto A, Diaz P, Mourazos N, Fernandez G, 2012. Seroprevalence and risk factors associated with visna/maedi virus in se- mi-intensive lamb-producing flocks in northwestern Spa- in. Prev Vet Med, 103, 163-169.
Lamara A, Fieni F, Chatagnon G, Larrat M, Dubreil L, Cheb- loune Y, 2013. Caprine arthritis encephalitis virus (CAEV) replicates productively in cultured epididymal cells from goats. Comp Immunol Microbiol Infect Dis, 36, 397-404.
Leroux C, Cruz JC, Mornex JF, 2010. SRLVs: A genetic continu- um of lentiviral species in sheep and goats with cumulati- ve evidence of cross species transmission. Curr HIV Res, 8, 94-100.
Li Y, Zhou F, Li X, Wang J, Zhao X, Huang J, 2013. Development of TaqMan-based qPCR method for detection of capri- ne arthritis-encephalitis virus (CAEV) infection. Arch Virol, 158, 2135-2141.
McNeilly TN, Tennant P, Lujan L, Perez M, Harkiss GD, 2007. Differential infection efficiencies of peripheral lung and tracheal tissues in sheep infected with visna/maedi virus via the respiratory tract. J Gen Virol, 88, 670-679.
Olech M, Rachid N, Croise B, Kuzmak J, Valas S, 2012. Genetic and antigenic characterization of small ruminant lentiviru- ses circulating in Poland. Virus Res, 163, 528-536.
Pekelder JJ, Veenink GJ, Akkermans JP, van Eldik P, Elving L, Houwers DJ, 1994. Ovine lentivirus induced indurative lymphocytic mastitis and its effect on the growth of lambs. Vet Rec, 134, 348-350.
Peterhans E, Greenland T, Badiola J, Harkiss G, Bertoni G, Amorena B, Eliaszewicz M, Juste RA, Krassnig R, La- font JP, Lenihan P, Petursson G, Pritchard G, Thorley J, Vitu C, Mornex JF, Pepin M, 2004. Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) in- fection and eradication schemes. Vet Res, 35, 257-274.
Pisoni G, Bertoni G, Boettcher P, Moroni P, 2004. Phylogene- tic analysis of the gag region encoding the matrix protein of small ruminant lentiviruses: comparative analysis and molecular epidemiological applications. Vet Res, 116, 159-167.
Reina R, Berriatua E, Lujan L, Juste R, Sanchez A, de Andres D, Amorena B, 2009. Prevention strategies against small ru- minant lentiviruses: An update. Vet J, 182, 31-37.
Rowe JD, East NE, 1997. Risk factors for transmission and methods for control of caprine arthritis-encephalitis virus infection. Vet Clin North Am Food Anim Pract, 13, 35-53.
Shah CA, Boni J, Huder JB, Vogt HR, Muhlherr J, Zanoni R, Mi- serez R, Lutz H, Schupbach J, 2004. Phylogenetic analysis and reclassification of caprine and ovine lentiviruses ba- sed on 104 new isolates: evidence for regular sheep-to- goat transmission andworld-wide propagation through livestock trade. Virology, 319, 12-26.
Tageldin MH, Johnson EH, Al-Busaidi RM, Al-Habsi KR, Al- Habsi SS, 2012. Serological evidence of caprine arthritis- encephalitis virus (CAEV) infection in indigenous goats in the Sultanate of Oman. Trop Anim Health Prod, 44, 1-3.
Tolari F, Al-Ramadneh W, Mazzei M, Carrozza ML, Forzan M, Bandecchi P, Grego E, Rosati S, 2013. Small ruminant lentiviruses in Jordan: evaluation of sheep and goat sero- logical response using recombinant and peptide antigens. Trop Anim Health Prod, 45, 1335-1340.
White SN, Knowles DP, 2013. Expanding possibilities for in- tervention against small ruminant lentiviruses through ge- netic marker-assisted selective breeding. Viruses, 5, 1466-1499.
Vitu C, Russo P, 1988. Caprine enzootic arthritis-encephali- tis in France: Epidemiological and experimental studies. Comp Immunol Microbiol Infect Dis, 11, 27-34.
Yapıcı O, Avcı O, Dik I, Atlı K, Yavru S, 2013. Saanen keçile- rinde Caprine Arthritis-Encephalitis Virus enfeksiyonunun serolojik araştırılması. AVKAE Derg, 3, 51-54.
Yavru S, Şimşek A, Kale M, Bulut O, 2006. An investigation of caprine arthritis encephalitis virus infection in goats. Vete- rinarium, 17, 4-7.