Çiğdem YENİSEY, Kamil SEYREK, Hakkı ÜNLÜ, Osman Selçuk ALDEMİR, Hasan EREN

Investigation on abortion mechanism of ovine sarcosporidiosis

Amaç: Bu çalışmada koyun sarcosporidiosis enfeksiyonu ile prostaglandin F2α F2 düzeyi aralarındaki ilişki incelenerek abort mekanizması üzerindeki etkisi açıklanmaya çalışılmıştır.Gereç ve Yöntem: Materyaller Aydın bölgesi mezbahanelerinden elde edildi. Parazit izolasyonu amacıyla PCR ve modifiye tripsin tekniği kullanıldı. Ayrıca kan örneklerindeki prostaglandin F2α düzeyleri ticari kit yardımı ile enzimimmunoassay (EIA) yöntemiyle belirlendi.Bulgular: İncelenen 114 koyunun 9 (%7.89)'unda Sarcocystis spp. makro kistleri, 58 (%55.23)'inde mikro kistler tespit edildi. Saptanan mikro kistlerin dağılımı ise 32 (%30.47)'sinde S. tenella, 17 (%16.19)'sinde S. gigantae ve 9 (%8.57)'unda S. arieticanis olarak belirlendi. Ayrıca 114 koyundan alınan kan örneklerinden 9 (%7.89) örnekte 300 ile 402 pg/mL, 47 (%41.22) örnekte 200 ile 300 pg/mL ve geri kalan örneklerde ise 53 ile 200 pg/mL arasında değişen değerlerde prostaglandin F2α düzeyi belirlendi. Gruplar arası fark istatistiksel olarak anlamlı bulundu (P

Koyun sarcosporidiozis enfeksiyonunda abort oluşum mekanizmasının araştırılması

Aim: In this study, the relationship between the level of prostaglandin F2α and ovine sarcosproridiosis infection were investigated for abortion mechanism.Materials and Methods: Materials were collected at slaughterhouse of Aydın region. In order to identification of parasite, macroscopical and microscopical cysts were used for S. gigantea and S. arieticanis or S. tenella by the PCR and Modified Trypcine Technique. Also in order to determination of the level of prostaglandin F2α in blood samples were used the methods of enzimmunoassay using a commercial kit (EIA).Results: A total number of 114 sheep oesophageal muscle were found in 9 (7.89%) to be infected with Sarcocystis macroscopic cysts. Examinations of a total number of 114 sheep were found in 58 (55.23%) of the sheep with microscopic cyst. These microscopic cyst species corresponding values were as follows: 32 (30.47%), 17 (16.19%) and 9 (8.57%) for S. tenella, S. gigantae and S. arieticanis, respectively. In addition a total number of 114 sheep blood examinations were determined in 9 (7.89%) samples at 300 and 402 pg/mL, 47 (41.22%) samples at 200 to 300 pg/mL and 58 (50.87%) samples at 53-200 pg/mL ranging between the levels of prostaglandin F2α. The differences between groups were found statistically significant (P

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