Hasan Hüseyin HADİMLİ, Gökçenur SANİOĞLU GÖLEN², Zafer SAYIN

Farelerde sığır immünglobulin G’sinin Salmonella enfeksiyonlarına karşı etkinliği

.Amaç: Bu çalışmada, farelerde Salmonella Dublin, S. Typhimurium, S. Kentucky veya S. Anatum tarafından oluşturulan Salmonella enfeksiyonlarına karşı sığır immünglobulin G'sinin (IgG) etkinliğinin belirlenmesi amaçlandı. Gereç ve Yöntem: Sığırlar inaktif monovalan, bivalan ve kombine Salmonella aşıları ile 21 gün arayla 3 kez aşılandılar. Son aşılamadan 10 gün sonra, aşılanan sığırlardan kan örnekleri toplandı ve Salmonella antijenlerine karşı şekillenen antikor titreleri mikro Serum Aglütinasyon testi ile belirlendi. Toplam 100 fareye (toplam 10 grup ve her grupta 10 fare) Salmonella türleri ile oral yolla çelınç yapıldı. Çelınç sonrası, farelere oral yolla IgG verildi. Fareler 20 gün boyunca hastalık oluşumu ve ölüm yönünden gözlendi. Aynı zamanda, dışkı ile Salmonella türlerinin saçılımı üzerine IgG'nin etkisini belirlemek için dışkı örnekleri analiz edildi. Bulgular: Sığır kanlarında aşılama sonrası oluşan antikor titrelerinin aşılama öncesi ve kontrollere göre yüksek olduğu bulundu. Aşı gruplarında hastalık oluşumu ve ölüm sayıları kombine aşı grubunda 9 (6 S. Dublin, 2 S. Typhimurium ve 1 S. Kentucky), bivalan aşı grubunda 3 (3 S. Typhimurium) ve monovalan aşı grubunda 2 (1'er S. Anatum ve S. Kentucky) olarak belirlendi. Bütün aşı gruplarında farelerin iç organlarından ve dışkı örneklerinden Salmonella türlerinin geri izolasyon sayıları düşük bulundu.Öneri: Sonuç olarak, Salmonella enfeksiyonlarına karşı sığır IgG'sinin koruyucu olarak faydalı ancak sınırlı bir etkinliğinin olduğu gözlendi.

Effectiveness of bovine immunoglobulin G against Salmonella infections in mice

Hadimli HH, Gölen GS, Sayın Z Effectiveness of bovine immunoglobulin G against Salmonella infections in miceAim: This study was aimed to determination of effectiveness of the bovine Immuneglobuline G (IgG) against Salmonella infections caused by Salmonella Dublin, S. Typhimurium, S. Kentucky or S. Anatum. Materials and Methods: Cattle were immunized with inactive monovalent, bivalent and combined Salmonella vaccines, three times at 21 days intervals. Immunized cattle blood samples were collected at 10 days after last vaccination and titres of IgG to Salmonella antigens were measured micro Serum Agglutination Test. A total of 100 mice (total 10 vaccine groups and each group included 10 mice) were challenged with Salmonella strains by oral route. After challenge, IgG to mice were administrated by oral route. Mice were observed for occurence of morbidity and mortality for 20 days. Also, feacal samples were analyzed to determination influence of IgG on spread of Salmonella species by feaces. Results: The antibodies titres in blood of immunized cattle were found to be high than before vaccination and controls. The numbers of morbidity and mortality in groups of vaccine were determined as 9 in combined vaccine (6 in S. Dublin, 2 in S. Typhimurium and 1 in S. Kentucky), 3 in bivalent vaccine (in S. Typhimurium), and 2 in monovalent (1 in S. Anatum and S. Kentucky). The re-isolation number of Salmonella strains were lower from internal organs and feacal samples of mice in all groups. Conclusions: In conclusion, it was observed that IgG of immunized cattle is can be useful for protection against Salmonella infections, but limited

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Ahmad A, Rahman S, Ahmad Z, Bilal M, Rabbani M, Muhammad K, 2011. Passive immunization of Pasteurella multocida infected rabbits. Pakistan J Zool 43, 623-628.
Aissa RB, Al-Gallas N, Troudi H, Belhadj N, Belhadj A, 2007. Trends in Salmonella enterica serotypes isolated from human, food, animal and environment in Tunisia, 1994-2004. J Infect 55, 324-339.
Barman S, Koley H, Naq D, Shinoda S, Nair GB, Takeda Y, 2014. Passive immunity with multi-serotype heat-killed Shigellae in mice. Microbiol Immunol 58, 463-466.
Berge ACB, Thornburg E, Adaska JM, Moeller RB, Blanchard PC, 2008. Antimicrobial resistance in Salmonella enterica subspecies enterica serovar Dublin from dairy source calves in the central San Joaquin Valley, California (1998– 2002). J Vet Diagn Invest 20, 497-500.
Besser T, Gay CC, McGuire TC, Evermann JF, 1988. Passive immunity to bovine rotavirus infection associated with transfer of serum antibody into the intestinal lumen. J Virol 62, 2238-2242.
Bock HL, Milcke-Ungehever L, 1988. Active and passive rabies immunization: The effect of administiring hyperimmune globulin before the vaccine. J Biol Standard 16, 67-69.
Casadevall A, 2002. Passive antibody administration (immediate immunity) as a specific defense against biological weapons. Emerg Infect Dis 8, 833-839.
Chiwerge M, Tyler JW, 2010. Serum IgG concentrations after intravenous serum transfusion in a randomized clinical trial in dairy calves with inadequate transfer of colostral immunoglobulins. J Vet Intern Med 24, 231-234.
Erganiş O, Corlu M, Hadimli HH, Kav K, 2003. Development and therapeutic effects of inavtivated Salmonella And Klebsiella vaccine for beef cattle: A preliminary report. Condition and Perspectives of Veterinary Science and Practice Development Materials of Int. Scientific and Research Conference, Almaty, Kazakistan, pp 96-100.
Figueiredo HCP, Lage, AP, Pereira Júnior FN, Leite, RC, 2004. Passive immunity in cattle against enterotoxigenic Escherichia coli: serologic evaluation of a bacterin containing K99 and F41 fimbriae in colostrum of vaccinated females and calf serum. Arq Bras Med Vet Zootec 56, 425-432.
Gupta M, Mahanty S, Bray M, Ahmed R, Rollin PE, 2001. Passive transfer of antibodies protects immunocompetent and immunodefiencient mice against lethal Ebola Virus Infection without complete inhibition of viral replication. J Virol 75, 4649-4654.
Hadimli HH, Erganiş O, Sayın Z, Yıldırım B, 2007. Fare ve koyunlarda ginseng katılmış inaktif Salmonella Typhimurium aşılarının etkinliği. Vet Bil Derg 23, 17-24.
Hadimli HH, Pınarkara Y, Al-Shattrawi HJ, 2014. Farelerde Salmonella İzolatlarının Patojenitelerinin Belirlenmesi, XI. Veteriner Hekimleri Mikrobiyoloji Kongresi (Uluslar arası Katılımlı), Kemer, Antalya, sf 20-21.
Hadimli HH, Sayın, Z, Erganiş O, 2011. Buzağılarda Salmonella Dublin Enfeksiyonu ve Otojen Aşı Uygulaması ile Kontrolü. Eurasian J Vet Sci 27, 99-105.
Herrmann JE, Wang S, Zhang C, Panchal RG, Bavari S, Lyons CR, Louchik JA, Golding B, Shiloach J, Lu, S, 2006. Passive immunotherapy of Bacillus anthracis pulmonary infection in mice with antisera produced by DNA immunization. Vaccine 24, 5872-5882.
Ikemori Y, Ohta M, Umeda K, Icatlo Jr FC, Kuroki M, Yokoyama H, Kodama Y, 2007. Passive protection of neonatal calves against bovine coronavirus-induced diarrhea by administration of egg yolk or colostrum antibody powder. Vet Microbiol 58, 105-111.
Jayappa H, Davis R, Dierks L, Sweeney D, Wasmoen T, 2008. Demonstration of passive protection in neonatal calves against colibacillosis following immunization of pregnant heifers at 3 months of gestation. Vet Therp 9, 283-289.
Jones PW, Collins P, Aitken MM, 1988. Passive protection of calves against experimental infection with Salmonella Typhimurium. Vet Rec 123, 536-541
Kalender H, 2002. Laboratuvar hayvanlarından Salmonella ssp. izolasyonu. İstanbul Univ Vet Fak Derg 28, 2.
Kalenik B, Sawicka R, Gora-Sochacka A, 2014. Influenza prevention and treatment by passive immunization. Acta Bioch Polonica 61, 573-587.
Keller MA, Stiehm ER, 2000. Passive immunity in prevention and treatment of infectious disease. Clin Microbiol Rev 13, 602-614.
Lewis PJ, van Drunen Littel-van den Hurk S, Babiuk LA, 1999. Induction of immune responses to bovine herpesvirus type 1 gD in passively immune mice after immunization. J Gen Virol 80, 2829-2837.
Maddoks S, Olma T, Chen S, 2002. Comparison of CHROM agar Salmonella medium and xylose- lysine- desoxycholate and Salmonella Shigella agars for isolation of Salmonella strains from stool samples. J Clin Microbiol 40, 2999-3003.
Mousavi M, Behrouz B, Irajian G, Mahdavi M, Korpi F, Motamefidar M, 2016. Passive immunization against Pseudomonas aeruginosa recombinant PilA in a murine burn wound model. Microbial Path 101, 83-88.
Muhamudana K, Madhusudana SN, Ravi V, 2007. Use of neutralizing murine monoklonal antibodies to rabies glycoprotein in passive immunotherapy against Rabies. Human Vaccines 3, 192-195.
Navarro DE, Baselga C, Sun D, Khairallah C, Mann I, Hernando UA, Sheridan B, Shi M, Fries BC, 2017. Antibody based immunotherapy to treat and prevent infection with hypervirulent Klebsiella pneumoniae. Clin Vaccine Immunol (in press), doi: 10.1128/CVI.00456-16
Rice DH, Besser TE, Hancock DL, 1997. Epidemiology and virulence assessment of Salmonella Dublin. Vet Microbiol 56, 111-124.
Smith GW, Smith F, Zuidhof S, Foster DM, 2015. Short communication: Characterization of the serologic response induced by vaccination of late-gestation cows with a Salmonella Dublin vaccine. J Dairy Sci 98, 2529-2532.
Smith-Palner A, Stewart WC, Mather H, Greig A, Cowden A, Cowden JM, Reilly WJ, 2003. Epidemiology of Salmonella enterica serovars Enteritidis and Typhimurium in animals and people in Scotland between 1990-2001. Vet Rec 153, 517-520.
Toye P, Handel I, Gray J, Kiara H, Thumbi S, Jennings A, van Wyk IC, Ndila M, Hanotte O, Coetzer K, Woolhouse M, Bronsvoort M, 2013. Maternal antibody uptake, duration and influence on survival and growth rate in a cohort of indigenous calves in a smallholder farming system in western Kenya. Vet Immun Immunopathol 155, 129-134.
Wang Y, Xiang Z, Pasquini S, Ertl HC, 1998. Effect of Passive immunization or maternally transferred immunity on the antibody response to a genetic vaccine to Rabies virus. J Virol 72, 1790-1796.
Zarcula S, Cernescu H, Knop R, 2008. Colostral immünity in newborn calf: Methods for improvement of immunoglobulins absorption. Luc Stiint Vet Med XLI, 195-202.
Zhan Y, Wang C, Qiu B, Li C, Way H, Jin H, Gai W, Zhang X, Wang T, Sun W, Yan F, Gao Y, Wang Q, Yan J, Chen L, Perlman S, Zhong N, Zhao J, Yang S, Xia X, 2017. Passive immunotherapy for Middle East Respiratory Syndrome coronavirus infection with equine immunoglobulin or immunoglobulin fragments in a mouse model. Antiviral Res 137, 125-130.