Kapllan SULAJ², Enver BAJRAMİ

Contamination with Escherichia coli of homemade fresh butter in rural areas of Ferizaj and Gjilan in Kosovo.

. Amaç: Kosova'da bazı kırsal bölgelerde üretilen taze ve ev yapımı tereyağının E.coli ile kontaminasyon düzeyinin belirlenmesi amaçlandı.Gereç ve Yöntem: 187 tereyağı örneği, 5-bromo-4-chloro-3indolyl- ß-D-glucuronic acid (BCIG) agar besi yerlerinde sulandırılarak analiz edildi. Daha ileri identifikasyon, farklı besi yerlerine ekimler ve biyokimyasal testler ile yapıldı ve E.coli izolatlarının identifikasyonunda API 20E stripleri kullanıldı.Bulgular: Bu araştırma 2014 yılında Kosova'da Gjilan ve Ferizaj kırsal bölgelerinde yaşayan ailelerden toplanan taze tereyağı örneklerinde gerçekleştirildi ve ?-glucuronidasepositif E. coli sayıları belirlendi. Çalışma ile tereyağı örneklerinin % 34 (64/187)'ünde E.coli kontaminasyonu belirlendi. Ferizaj bölgesinde E.coli izolasyonu % 22 (20/189) oranı ile yüksekti. Toplamda 187 tereyağı örneğinin 36 (%19)'ının 5 log cfu/g'den yüksek E.coli içerdiği tespit edildi.Öneriler: Taze tereyağı Kosova'nın kırsal alanlarında çoğu aile tarafından ev yapımı metot üretilen bir süt ürünüdür. Geleneksel yöntem ile üretilen taze tereyağı çoğunlukla patojen E.coli suşlarını da içeren çeşitli mikroorganizmalar ile kontamine olur. Kosova'nın bu kırsal alanlarında zayıf hijyenik koşullar altında ev üretiminin tereyağının kalitesine etkisi vardır ve zayıf hijyen tereyağında artan sayıdaki E.coli ile ilişkilidir.

Kosova’nın Ferizaj ve Gjilan kırsal bölgelerinde üretilen ev yapımı taze tereyağlarının E.coli ile kontaminasyonu

Bajrami E, Sulaj K. Contamination with Escherichia coli of homemade fresh butter in rural areas of Ferizaj and Gjilan in Kosovo. Aim: It was aimed to evaluate level of contamination of Escherichia coli in homemade fresh butter in some rural areas in Kosovo.Materials and methods: A hundred eighty seven butter samples were analyzed inoculating diluted volumes in selective agar plates containing 5-bromo-4-chloro-3-indolyl- ß-Dglucuronic acid (BCIG). The further identification of isolates was performed using other media and some additional biochemical tests as well as API 20E strips are used for identification of E. coli isolates. Results: This research was carried out in 2014 to evaluate the number of β-glucuronidase-positive Escherichia coli in homemade fresh butter samples collected by families in rural areas of regions; Gjilan and Ferizaj in Kosovo. This analytical check concluded that 34% (64/187) of fresh butter samples were contaminated with E. coli. The high number of E. coli was detected in 22% (20/189) butter samples collected in rural areas of Ferizaj. In total, 36 out 187 butter samples or 19% showed values of E. coli higher than 5log cfu/g. Conclusions: Homemade fresh butter produced from fresh cow’s milk in rural areas of Kosovo is commonly contaminated with different kinds of microorganisms as well as by pathogenic strains of E. coli. The poor hygienic home conditions in some rural areas in Kosovo have impact in the butter quality and are associated with the increasing number of E. coli

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