Zafer SAYIN, Yasemin PINARKARA, Aslı SAKMANOĞLU, M. Seyyide TEMİMHAN, Osman ERGANİŞ, Hasan Hüseyin HADİMLİ

Farelerde inaktif Arcanobacterium pyogenes aşılarının etkinliği

Amaç: Bu çalışmada, mineral oil (MO) ve alüminyum hidroksit + ginseng [Al(OH3) + G] ile adjuvantlanmış inaktif Arcanobacterium pyogenes aşılarının farelerde etkinliğinin belirlenmesi amaçlandı. Gereç ve Yöntem: İnaktif A. pyogenes aşıları, farelere 3 hafta aralıkla 2 kez deri altı yolla uygulandı. Aşılı ve aşısız fareler 2. aşılamadan 15 gün sonra canlı patojenitesi LD50 olan A. pyogenes suşu deri altı yolla verilerek çelınç yapıldı. Fareler 20 gün boyunca ölüm ve hastalık yönünden gözlendi. Ölen hayvanların nekropsisi yapıldı ve iç organlarının (dalak, karaciğer, akciğer, kalp, böbrek) mikrobiyolojik yoklamaları gerçekleştirildi. Aşılama öncesi ve sonrası 15. günde kan örnekleri alındı. Serum örneklerinde A. pyogenes’e karşı oluşan antikor titreleri modifiye Enzyme Linked Immunosorbent Assay (ELISA) ile belirlendi. Bulgular: Kontrollere göre aşılanmış fare serumlarındaki antikor titreleri belirgin olarak yüksek bulundu. Aşılı ve çelınç yapılmış farelerde ölüm ve morbidite gözlenmezken, kontrol grubu farelerde hastalık ve ölüm belirlendi. Öneri: A. pyogenes izolatından hazırlanan A. pyogenes aşılarının farelerde A. pyogenes enfeksiyonlarından korunmada faydalı olduğu bulundu.

The effectiveness of inactive Arcanobacterium pyogenes vaccines in mice.

Aim: The aim of this study was to determine effectiveness of inactivated Arcanobacterium pyogenes vaccines adjuvanted with mineral oil (MO) and aluminum hydroxide + ginseng [Al(OH3) + G] in mice. Materials and Methods: Inactive A. pyogenes vaccines were subcutaneously administered to mice twice at 3 weeks intervals. Mice were subcutaneously challenged with live A. pyogenes according to LD50 at 15th day after second vaccination. Mice were observed for mortality and morbidity during 20 days. Death animals were necropsied and microbiological examination were made from internal organs (liver, spleen, lungs, heart and kidney). Blood samples were collected before vaccination and at 15th day after vaccination. The titers of antibodies against A. pyogenes in serum samples were detected by using self-made modified Enzyme Linked Immunosorbent Assay (ELISA). Results: Levels of antibodies in sera of vaccinated animals were significantly higher than the controls. While no mortality and morbidity were observed in vaccinated and challenged mice, both morbidity and mortality were determined in non-vaccinated mice. Conclusion: Inactive A. pyogenes vaccines prepared from local A. pyogenes strain was found to be useful to protect mice from the infection of A. pyogenes.

PDF

___

Billington SJ, Jost BH, Cuevas WA, Bright KR, Songer JG, 1997. The Arcanobacterium (Actynomyces) pyogenes hemolysin, pyolysin, is a novel member of the thiolactivated cytolysin family. J Bacteriol, 179, 6100-6106.
Cho YS, Lee HS, Lim SK, Joo YS, Kim JM, Kim JH, 2008. Safety and efficacy testing of a novel multivalent bovine bacterial respiratory vaccine composed of five bacterins and two immunogens. J Vet Med Sci, 70, 959-964.
Dias CAG, Cauduro PF, Mezzari A, Cantarelli V, 1996. Actynomyces pyogenes isolated from a subcutaneous abcess in a dairy farmer. Clin Microbiol Lett, 18, 38-40.
Ding H, Lammler C, Vecht U, 1998. Measurement of Actinomyces pyogenes specific antibodies in bovine blood samples by an enzyme-linked immunosorbent assay. Zentralbl Veterinarmed B, 45, 297-303.
Ertaş HB, Kılıç, A, Özbey G, Muz A, 2005. Isolation of Arcanobacterium (Actynomyces) pyogenes from abcessed kidney and identification by PCR. Turk J Vet Anim Sci, 29, 455-459.
Esmay PA, Billington SJ, Link MA, Songer JG, Jost BH, 2003. The Arcanobacterium pyogenes collagen-binding protein, Cbp A, promotes adhesion to host cells. Infect Immun, 71, 4368-4374.
Gouletsou PG, Ethenakis GC, Tzora A, Cripps PJ, Saratsis P, 2006. Isolation of Arcanobacterium pyogenes from the scrotal skin and the prepuce of healthy rams or from rams with testicular abnormalities. Small Rumin Res, 63, 177-182.
Hadimli HH, Erganiş O, Kav K, Sayın Z, 2010. Koyun ve sığır örneklerinden Arcanobacterium pyogenes izolasyonu ve polimeraz zincir reaksiyonu ile identifikasyonu. Kafkas Univ Vet Fak Derg, 16, 611-616.
Hadimli HH, Kav K, 2011. The molecular characterization of Arcanobacterium pyogenes strains isolated from samples of sheep and cattle. Kafkas Univ Vet Fak Derg, 17, 893-899.
Hadimli HH, Erganiş O, Sayın Z, Yıldırım B, 2007. Fare ve Koyunlarda ginseng katılmış inaktif Salmonella typhimurium aşılarının etkinliği. Eurasian J Vet Sci, 23, 17-24.
Hadimli HH, Sayın Z, Kav K, Erganiş O, Türütoğlu H, Dinç DA, 2011a. The Determination of effectiveness combined mastitis prepared vaccines for dairy cows in mice. 3th East Mediterranean ICLAS Symposium & XV. ICLAS Genaral Assembly. June 13-15, pp: 52, Istanbul, Turkey.
Hadimli HH, Sayın Z, Kav K, Erganiş O, Türütoğlu H, Dinç DA, 2011b. The Development of Combined Mastitis Vaccines for Different Bacterial Agents (Staphlylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Corynebacterium bovis and Arcanobacterium pyogenes) in Dairy Cows. 19. International Congress of Mediterranean Federation of Health and Production of Ruminants, pp: 461, May 25-28, Belgrade, Serbia.
Hijazin M, Ülbegi-Mohyla H, Alber J, Lämmler C, Hassan AA, Abdulmawjood A, Prenger-Berninghoff E, Weib R, Zschöck M, 2011. Molecular identification and further characterization of Arcanobacterium pyogenes isolated from bovine mastitis and from various other origins. J Dairy Sci, 94, 1813-1819.
Hunter P, van der Lugt JJ, Gouws JJ, 1990. Failure of an Actinomyces pyogenes vaccine to protect sheep against an intravenous challenge. Onderstepoort J Vet Res, 57, 239-41.
Jost BH, Billington SJ, 2005. Arcanobacterium pyogenes: Molecular pathogenesis of an animal opportunist. Antonie van Leeuwenhoek, 88, 87-102.
Jost BH, Songer JG, Billington SJ, 1999. An Arcanobacterium (Actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin pyolysin has reduced virulence. Infect Immun, 67, 1723-1728.
Jost BH, Songer JG, Billington SJ, 2002. Identification of a Second Arcanobacterium pyogenes neuraminidase and Involvement of neuraminidase activity in host cell adhesion. Infect Immun, 70, 1106-1112.
Jost BH, Trinh HT, Songer JH, Billington SJ, 2003. Immunization with genetic toxoids of the Arcanobacterium pyogenes cholesterol-dependent cytolysin, pyolysin, protects mice against infection. Infect Immun, 71, 2966-2969.
Madsen M, Sorensen GH, Aalbaek B, Hansen JW, Bjorn H, 1992. Summer mastitis in heifers: Studies on the seasonal occurrence of Actinomyces pyogenes, Peptostreptococcus indolicus and bacteroidaceae in clinically healthy cattle in Denmark. Vet Microbiol, 30, 243-255.
Miller ANA, Williams EJ, Sibley K, Herath S, Lane EA, Fishwick J, Nash DM, Rycroft AN, Dobson H, Bryant CE, Sheldon IM, 2007. The effects of Arcanobacterium pyogenes on endometrial function in vitro, and on uterine and ovarian function in vivo. Theriogenol, 68, 972-980.
Morrison JRA, Tillotson, GS, 1988. Identification of Actinomyces (Corynebacterium) pyogenes with the API 20 Strep system. J Clin Microbiol, 26, 1865-1866.
Nagaraja TG, Chengappa MM, 1998. Liver abscesses in feedlot cattle: A review. J Anim Sci, 76, 287-298.
Nolte O, Morscher J, Weiss HE, Sonntag HG, 2001. Autovaccination of dairy cows to treat post partum metritis caused by Actinomyces pyogenes. Vaccine, 19, 3146-3153.
Quinn AK, Vermont JJ, Twiss DP, 2002. Arcanobacterium pyogenes mastitis in a 18-month-old heifer. New Zealand Vet J, 50, 167-168.
Semembo DKN, TR, Ayliffe J, Boyd S, Taylor DJ, 1991. Early abortion in cattle induced by experimental intrauterine infection with pure cultures of Actinomyces pyogenes. Vet Rec, 129, 12-16.
Silva E, Gaiva M, Leita S, Jost BH, Carneiro C, Vilela CL, Lopes da Costa L, Mateus L, 2008. Genomic characterization of Arcanobacterium pyogenes isolates recovered from the uterus of dairy cows with normal puerperium or clinical metritis. Vet Microbiol, 25, 111-118.
Simpson RB, Wesen DP, Anderson KL, Armstrong JD, Harvey RW, 1995. Subclinical mastitis and milk production in primiparous Simmental cows. J Anim Sci, 73, 1552- 1558.
Wulster-Radcliffe MC, Seals RC, Lewis GS 2005. Uterine response to multiple inoculations with Arcanobacterium pyogenes and Escherichia coli in Nulliparous Ewes. Am J Reprod Immun, 54, 249-261.