Konya Merinosu toklularda aşımla birlikte gonadotropin-releasing hormon veya human chorionic gonadotropin uygulamasının kuzulama performansına etkisi

Amaç: Bu çalışma da Konya Merinosu toklularda aşımla birlikte gonadotropin-releasing hormon (GnRH) veya human chorionic gonadotropin (hCG) uygulamasının senkronizasyon periyodu ve izleyen siklusta yaptırılan aşımlar sonrası kuzulama performansına etkisinin belirlenmesi amaçlandı. Gereç ve Yöntem: Materyal olarak 16-20 aylık 36 baş Konya Merinosu toklu kullanıldı. Tokluların östrüsleri 0. gün 4 μg GnRH ve 5. gün 150 μg prostaglandinF2α (PGF2α) uygulanmasıyla senkronize edildi. PGF2α enjeksiyonu sonrası östrüste olduğu tespit edilen toklular fertil oldukları daha önceden bilinen koçlarla bir kez çiftleştirildi. Aşım sonrası toklular sürüden uzaklaştırıldı ve üç gruba ayrıldı. Birinci gruptaki toklulara (n=14) 200 IU hCG, ikinci gruptakilere (n=12) 4 μg GnRH ve üçüncü gruptakilere (kontrol, n=10) 1 mL serum fizyolojik kas içi enjekte edildi. Gebelik muayeneleri aşım sonrası 30. günde ultrasononografi cihazı kullanılarak transrektal yoldan yapıldı. Bulgular: Sunulan çalışmada gebe kalan, doğum yapan, ikiz doğum yapan koyun oranı ve kuzu verimi sırasıyla hCG grubunda %85.71, %85.71, %33.3 ve 1.14, GnRH grubunda %75.0, %66.67, %12.5 ve 0.75, kontrol grubunda ise %100, %80, %37.5 ve 1.10 olarak belirlendi. Gruplar arasında kuzulama performansı ve incelenen reprodüktif parametreler arasındaki farklar istatistiki açıdan önemli bulunmadı (p>0.05). Öneri: Sonuç olarak östrüsleri senkronize edilen toklularda aşımla birlikte GnRH veya hCG uygulamalarının kuzulama performansı üzerine etkisinin olmadığı kanaatine varıldı.

The effect of gonadotropin- releasing hormone or human chorionic gonadotropin administration at mating on lambing performance in Konya Merino yearling ewes.

Aim: In this study, it was aimed to determine the effects of the exogenous gonadotropin-releasing hormone (GnRH) or human chorionic gonadotropin (hCG) application at mating on lambing performance in synchronization and following cycle in Konya Merino yearling ewes. Materials and Methods: Thirty six yearling Merino ewes, aging 16-20 months, were used as material. Estrus of ewes was synchronized with 4 μg GnRH on d 0 and 150 μg prostaglandinF2α (PGF2α) on day 5, intramuscularly. After PGF2α injection, the ewes detected to be in estrus were mated with a ram, whose fertility was known previously, and they were divided into three groups. Group I (n=14) were injected 200 IU hCG, Group II (n=12) were injected 4 μg GnRH, and Group III (control group, n=10) were injected 1 mL placebo at mating. Pregnancy was diagnosed on day 30 by using transrectal ultrasonography. Results: In the present study, pregnancy, birth, twinning rate and lambing performance were 85.71%, 85.71%, 33.3% and 1.14 in hCG group, 75.0%, 66.67%, 12.5%, and 0.75 in GnRH group, and 100%, 80%, 37.5% and 1.10 in control group, respectively. There was no significant difference among groups for lambing performance and studied reproductive parameters (p>0.05). Conclusion: In conclusion, the injection of GnRH or hCG in Merino ewes at mating was not effective on lambing performance.

___

  • Bajaj NK, Sharma N, 2011. Endocrine causes of early embryonic death: An overwiev. Curr Res Dairy Sci, 3, 1-24.
  • Çam MA, Kuran M, 2004. Effects of a single injection of hCG or GnRH agonist on day 12 post mating on fetal growth and reproductive performance of sheep. Anim Reprod Sci, 80, 81-90.
  • Davies MCG, Beck NFG, 1993. Acomparison of plasma prolactin, LH and progesterone concentration during oestrus and early pregnancy in ewe lambs and ewes. Anim Prod, 57, 281-286.
  • De Rensis F, Valentini R, Gorrieri F, Bottarelli E, Lopez- Gatius F, 2008. Inducing ovulation with hCG improves the fertility of dairy cows during the warm season. Theriogenology, 69, 1077-1082.
  • De Rensis F, Lopez-Gatius F, I. Garcia-Ispierto I, Techakumpu M, 2010. Clinical use of human chorionic gonadotropin in dairy cows: An update. Theriogenology, 73, 1001- 1008.
  • Diskin MG, Morris DG, 2008. Embryonic and early foetal losses in cattle and other ruminants. Reprod Dom Anim, 43 (Suppl. 2), 260-267.
  • Erdem H, Sarıbay MK, Tekeli T, 2006. Aşım sezonunda östrüsleri senkronize edilen Konya Merinosu koyunlarda embriyonik ölümlerin real-time ultrason ile belirlenmesi. Hay Araş Derg, 16, 14-18.
  • Gomez-Brunet AG, Santiago-Moreno J, Montoro V, Garde J, Pons P, Gonzalez-Bulnes A, Lopez-Sebastian AL, 2007. Reproductive performance and progesterone secretion in estrus-induced Manchega ewes treated with hCG at the time of AI. Small Rum Res, 71, 117-122.
  • Gökçen H, Soylu MK, Doğan İ, 2000. Sıvı azotta dondurulan koç spermasının spermatolojik özellikleri ve değişk yöntemlerle tohumlamada kullanılması üzerine araştırmalar. Turk J Vet Anim Sci, 24, 539-544.
  • Ishida N, Okada M, Sebata K, Minato M, Fukui Y, 1999. Effects of GnRH and hCG treatments for enhancing corpus luteum function to ıncrease lambing rate of ewes artificially inseminated during the non-breeding season. J Reprod Dev, 45, 73-79.
  • Jadav PV, Patel DM, Kavani FS, Dhami AJ, 2010. GnRH and its applications in bovine reproduction. J Adv Dev Res, 1, 74-80.
  • Khan TH, Hastie PM, Beck NFG, Khalid M, 2003. hCG treatment on day of mating improves embryo viability and fertility in ewe lambs. Anim Reprod Sci, 76, 8189.
  • Khan TH, Beck NFG, Khalid M, 2009. The effect of hCG treatment on Day 12 post-mating on ovarian function and reproductive performance of ewes and ewe lambs. Anim Reprod Sci, 116, 162-168.
  • Lashari MH, Tasawar Z, 2010. The Effect of GnRH given on day of mating on ovarian function and reproductive performance in Lohi Sheep. Pakistan Vet J, 30, 29-33.
  • Michels H, Vanmontfort D, Dewil E, Decuypere E, 1998. Genetic variation of survival in relation to ovulation rate in sheep. Small Rum Res, 29, 129-142.
  • Nancarrow CD, 1994. Embryonic mortality in the ewe and doe, in; Embryonic mortality in Domestic Species, Eds; MT Zavy and RD Geisert, Crd Press, Boca Raton, pp: 79- 97.
  • Nephew KP, Cardenas H, McClure KE, Ott TL, Bazer FW, Pope WF, 1994. Effects of administration of human chorionic gonadotropin or progesterone before maternal recognition of pregnancy on blastocyst development and pregnancy in sheep. J Anim Sci, 72, 453-458.
  • Peters AR, 2005. Veterinary clinical application of GnRHquestinos of efficacy. Anim Reprod Sci, 88, 155-167.
  • Sarıbay MK, Erdem H, 2007. Koyunlarda real-time ultrasonografi ile embriyonik ölümlerin insidansının belirlenmesi. Eurasian J Vet Sci, 23, 19-25
  • Schneider F, Tomek W, Gründker C, 2006. Gonadotropinreleasing hormone (GnRH) and its natural analogues: A review. Theriogenology, 66, 691-709.
  • Thatcher WW, Staples CR, Danet-Desnoyers G, Oldick B, Schmitt EP, 1994. Embryo health and mortality in sheep and cattle. J Anim Sci, 72, 16-30.
  • Türk G, Gür S, Sönmez M, Bozkurt T, Aksu EH, Aksoy H, 2008. Effect of exogenous GnRH at the time of artificial ınsemination on reproductive performance of Awassi Ewes synchronized with progestagen-PMSG-PGF2α combination. Reprod Dom Anim, 43, 308-313.
  • Windsor DP, 1995. Factors influencing the success of transcervical insemination in merino ewes. Theriogenology, 43, 1009-1018.
  • Zamiri MJ, Hosseini M, 1998. Effects of human chorionic gonadotropin (hCG) and phenobarbital on the reproductive performance of fat-tailed Ghezel ewes. Small Rumin Res, 30, 157-161.
Eurasian Journal of Veterinary Sciences-Cover
  • ISSN: 1309-6958
  • Yayın Aralığı: Yılda 4 Sayı
  • Yayıncı: Selçuk Üniversitesi Veteriner Fakültesi