In vitro Investigation of Deoxyribonucleic Acid Interaction and Anti-Acetylcholinesterase Activity of Turnip (Brassica Rapa Subsp. Rapa)

Turnip (Brassica rapa subsp. Rapa) is a herbaceous and seasonal plant found in the cruciferous family. It is possible to grow in many regions of Europe and West Asia. Although many studies have been carried out to show that turnip juice obtained from turnip root has high biological activity, there has been no previous study on the interaction of turnip root with DNA or its anti-acetylcholinesterase activity. In this study, the interaction and anti-acetylcholinesterase activity of turnip, which obtained three different extracts by applying Soxhlet extraction, were tried to be determined. The DNA binding properties, DNA protective effects, DNA restorative effects and anti-acetylcholinesterase activity of the obtained water, ethanol and ethyl acetate extracts were calculated and the results obtained were compared with other plants of the same species in the past studies.

Keywords:

Turnip, DNA, DNA interaction, acetylcholinesterase Soxhlet extraction, natural product,

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Archana, J., Kusuma, M. P., & Vijayabhargavi, C. (2018). Antioxidant and Anti Cholinesterase Potential of Red Cabbage (Brassica oleracea var. capitata f. rubra). European Journal of Medicinal Plants, 22(1), 1-7. DOI: doi.org/10.9734/EJMP/2018/38169
Butler, J.M., (2001). Forensic DNA Typing (1st edition), New York: Elsevier. pp. 99-140.
Hasanzadeh, M. ve Shadjou, N., (2016). "Pharmogenomic study using bio and nanobioelectrochemistry: Drug-DNA interaction", Materials Science and Engineering: C, 61: 1002-1017. DOI: doi.org/10.1016/j.msec.2015.12.020
Ingkaninan, K., Temkitthawon, P., Chuenchom, K., Yuyaem, T., Thongnoi, W., (2003). Screening for acetylcholinesterase inhibitory activity in plants used in Thai traditional rejuvenating and neurotonic remedies, Journal of Ethnopharmacology, 89 (2–3), 261-264. DOI: doi.org/10.1016/j.jep.2003.08.008
Katzung, B. G., (2001). Basic and clinical pharmacology: Introduction to autonomic pharmacology (8 ed.). The McGraw Hill Companies. pp. 75–91.
Larsen, N.B., Rasmussen, M. ve Rasmussen, L.J., (2005). "Nuclear and mitochondrial DNA repair: similar pathways?", Mitochondrion, 5 (2): 89-108. DOI: doi.org/10.1016/j.mito.2005.02.002
Lodish H., B.A., Matsudaira P., Kaiser C.A., Krieger M., Scott M.P., Zipursky S.L. ve Darnell J., (2004). Molecular Biology of the Cell (5th edition), New York: W. H. Freeman & Co Ltd. pp. 899-933.
Nelson, D.L., Cox, M.M. ve Lehninger, A.L., (2013). Principles of Biochemistry (5th edition), New York: W. H. Freeman & Co Ltd. pp. 273-302.
Nwidu, L. L., Elmorsy, E., Thornton, J., Wijamunige, B., Wijesekara, A., Tarbox, R., Warren, A., Carter, W. G., (2017). Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea, Pharmaceutical Biology, 55:1, 1875-1883. DOI: doi.org/10.1080/13880209.2017.1339283
Padilla, G., Cartea, M. E., Rodríguez, V. M. ve Ordás, A., (2005). "Genetic diversity in a germplasm collection of Brassica rapa subsp rapa L. from northwestern Spain", Euphytica, 145 (1-2): 171–180. DOI: doi.org/10.1007/s10681-005-0895-x
Rajendiran, V., Palaniandavar, M., Periasamy, V. ve Akbarsha, M., (2012). "New [Ru(5,6-dmp/3,4,7,8-tmp)2(diimine)]2+ complexes: Non-covalent DNA and protein binding, anticancer activity and fluorescent probes for nuclear and protein components", Journal of Inorganic Biochemistry, 116: 151-162. DOI: doi.org/10.1016/j.jinorgbio.2012.06.005
Yıldız, U., Çoban, B. ve Şengül, A., (2015). “Pt(bpy)(dicnq)]2+ Kompleksinin Sentezi ve DNA Etkileşimlerinin Belirlenmesi”, Bülent Ecevit University, Faculty of Arts and Science, Chemistry Department, Zonguldak, V. National Conference of Anorganic Chemistry.