Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı
Son yıllarda, pratik ve etik nedenlerle, ekotoksikolojide in vitro yöntemlere olan ilgi artmıştır. Sitotoksisite çalışmalarında hücre kültürü uygulamaları, in vivo çalışmalardan elde edilen sonuçlara uyumluluğu ve hücresel düzeyde inceleme olanağı gibi avantajlarıyla, diğer in vitro yöntemlere göre öne çıkmıştır. Balık hücre kültürleriyle yapılan çalışmalar, kimyasal maddelerin toksik ve çevresel etkilerinin değerlendirilmesine imkan sağlamaktadır. Ekotoksikolojide, kimyasal maddelerin toksik ve çevresel etkilerinin değerlendirilmesinde RTG-2, RTL-W1, PLHC-1 ve EPC gibi çeşitli balık hücre hatları sıklıkla tercih edilmektedir. Bu derlemede, balık hücre hatlarının ekotoksikoloji çalışmalarında kullanımları ve dikkat edilmesi gereken bazı hususlar ele alınmıştır.
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- 1. Acker LA, McMahan JR, Gawel JE, (2005). The effect of
heavy metal pollution in aquatic environments on metallothionein
production in Mytilus sp. Proceedings of the 2005
Puget Sound Georgia Basin Research Conference.
- 2. Ackermann GE, Brombacher E, Fent K, (2002).
Development of a fish reporter gene system for the assessment
of estrogenic compounds and sewage treatment plant
effluents. Environ Toxicol Chem. 21, 1864-1875.
- 3. Araujo CSA, Marques SAF, Carrondo MJT, Gonçalves
LMD, (2000). In vitro response of the brown bullhead catfish
(BB) and rainbow trout (RTG-2) cell lines to benzo(a)
pyrene. Sci Total Environ. 247, 127-135.
- 4. ATCC, (2018). Erişim adresi: https://www.lgcstandardsatcc.
org/en.aspx , Erişim tarihi: 09.11.2018.
- 5. Babin MM, Tarazona JV, (2005). In vitro toxicity of selected
pesticides on RTG-2 and RTL-W1 fish cell lines.
Environ Pollut. 135, 267-274.
- 6. Babin M, Boleas S, Tarazona JV, (2005). In vitro toxicity
of antimicrobials on RTG-2 and RTL-W1 fish cell lines.
Environ Toxicol Pharmacol. 20, 125–134.
- 7. Baby J, Raj JS, Biby ET, Sankarganesh P, Jeevitha MV,
Ajisha SU, Rajan SS, (2010). Toxic effect of heavy metals
on aquatic environment. Int J Biol Chem Sci. 4(4), 939-
952.
- 8. Behrens A, Schirmer K, Bols NC, Segner H, (2001).
Polycyclic aromatic hydrocarbons as inducers of cytochrome
P4501A enzyme activity in the rainbow trout liver
cell line, RTL-W1 and in primary cultures of rainbow trout
hepatocytes. Environ Toxicol Chem. 20(3), 632-643.
- 9. Birnbaum LS, Tuomisto J, (2000). Non-carcinogenic effects
of TCDD in animals. Food Addit Contam. 17(4),
275-288.
- 10. Bols NC, Dayeh VR, Lee LEJ, Schirmer K, (2005). Use of
fish cell lines in the toxicology and ecotoxicology of fish.
Piscine cell lines in environmental toxicology. Mommsen
TP, Moon TW. eds. Biochemistry and molecular biology
of fishes. Elsevier B.V. p.43-84.
- 11. Bols NC, Pham PH, Dayeh VR, Lee LEJ, (2017).
Invitromatics, invitrome, and invitroomics: introduction
of three new terms for in vitro biology and illustration of
their use with the cell lines from rainbow trout. In Vitro
Cell Dev Biol. 53, 383-405.
- 12. Brack W, Schirmer K, Kind T, Schrader S, Schüürmann G,
(2002). Effect-directed fractionation and identification of
cytochrome P4501A-inducing halogenated aromatic hydrocarbons
in a contaminated sediment. Environ Toxicol
Chem. 21(12), 2654–2662.
- 13. Caminada D, Escher C, Fent K, (2006). Cytotoxicity of
pharmaceuticals found in aquatic systems: Comparison
of PLHC-1 and RTG-2 fish cell lines. Aquat Toxicol. 79,
114-123.
- 14. Castano A, Bols N, Braunbeck T, Dierickx P, Halder M,
Isomaa B, Kawahara K, Lee LEJ, Mothersill C, Pärt P,
Repetto G, Sintes JR, Rufli H, Smith R, Wood C, Segner
H, (2003). The use of fish cells in ecotoxicology. ATLA.
31, 317-351.
- 15. Celander M, Hahn ME, Stegeman JJ, (1996). Cytochromes
P450 (CYP) in the Poeciliopsis lucida hepatocellular carcinoma
cell line (PLHC-1): Dose and time-dependent
glucocorticoid potentiation of CYP1A induction without
induction of CYP3A. Arch Biochem Biophys. 329(1), 113-
122.
- 16. Coyle P, Philcox JC, Carey LC, Rofe AM, (2002).
Metallothionein: The multipurpose protein. Cell Mol Life
Sci. 59, 627-647.
- 17. Dayeh VR, Schirmer K, Bols NC, (2002). Applying wholewater
samples directly to fish cell cultures in order to
evaluate the toxicity of industrial effluent. Water Res. 36,
3727-3738.
- 18. Fent K, (2001). Fish cell lines as versatile tools in ecotoxicology:
assessment of cytotoxicity, cytochrome P4501A
induction potential and estrogenic activity of chemicals
and environmental samples. Toxicol In Vitro. 15, 477-488.
- 19. Fent K, (2007). Permanent fish cell cultures as important
tools in ecotoxicology. ALTEX. 24(8), 26-28.
- 20. Fent K, Batscher R, (2000). Cytochrome P4501A induction
potencies of polycyclic aromatic hydrocarbons in a
fish hepatoma cell line: demonstration of additive interactions.
Environ Toxicol Chem. 19(8), 2047–2058.
- 21. Fent K, Hunn J, (1996). Cytotoxicity of organic environmental
chemicals to fish liver cells (PLHC-1). Mar
Environ Res. 42(14), 377-382.
- 22. IARC, (2010). Benzo[a]pyrene. https://monographs.iarc.
fr/wp-content/uploads/2018/06/mono100F-14.pdf ,Erişim
tarihi: 30.11.2018.
- 23. Kammann U, Bunke M, Steinhart H, Theobald N, (2001).
A permanent fish cell line (EPC) for genotoxicity testing of
marine sediments with the comet assay. Mutat Res Genet
Toxicol Environ Mutagen. 498(1-2), 67-77.
- 24. Kling P, Erkell LJ, Kille P, Olsson PE, (1996).
Metallothionein induction in rainbow trout gonadal
(RTG-2) cells during free radical exposure. Mar Environ
Res. 42(1-4), 33-36.
- 25. Lakra WS, Raja Swaminathan T, Joy KP, (2011).
Development, characterization, conservation and storage
of fish cell lines: a review. Fish Physiol Biochem. 37, 1-20.
- 26. Lange M, Gebauer W, Markl J, Nagel R, (1995).
Comparison of testing acute toxicity on embryo of zebrafish,
Brachydanio rerio and RTG-2 cytotoxicity as possible
alternatives to the acute fish test. Chemosphere. 30(11),
2087-2102.
- 27. Lee LEJ, Clemons JH, Bechtel DG, Caldwell SJ, Han
KB, Pasitschniak-Arts M, Mosser DD, Bols NC, (1993).
Development and characterization of a rainbow trout liver
cell line expressing cytochrome P450-dependent monooxygenase
activity. Cell Biol Toxicol. 9(3), 279-294.
- 28. Lee LEJ, Dayeh VR, Schirmer K, Bols NC, (2009).
Applications and potential uses of fish gill cell lines: examples
with RTgill-W1. In Vitro Cell Dev Biol. DOI:10.1007/
s11626-008-9173-2.
- 29. Li H, Zhang S, (2001). In vitro cytotoxicity of the organophosphorus
pesticide parathion to FG-9307 cells. Toxicol
In Vitro. 15, 643-647.
- 30. Llorente MT, Parra JM, Sanchez-Fortun S, Castano A,
(2012). Cytotoxicity and genotoxicity of sewage treatment
plant effluents in rainbow trout cells (RTG-2). Water Res.
46, 6351-58
- 31. Maracine M, Segner H, (1998). Cytotoxicity of metals in
isolated fish cells: Importance of the cellular glutathione
status. Comp Biochem Physiol A.120, 83–88.
- 32. Nehls S, Segner H, (2001). Detection of DNA damage in
two cell lines from rainbow trout, RTG-2 and RTL-W1, using
the Comet assay. Environ Toxicol. 16, 321-329.
- 33. Nehls S, Segner H, (2005). Comet assay with the fish cell
line rainbow trout gonad-2 for in vitro genotoxicity testing
of xenobiotics and surface waters. Environ Toxicol Chem.
(24)8, 2078–2087.
- 34. Parra J.M, Sanchez-Fortun S, Castano A, (2012).
Assessment of genotoxic effects induced by selected pesticides
on RTG-2 fish cells by means of a modified fast
micromethod assay. Environ Toxicol. 27, 238–243.
- 35. Rachlin JW, Perlmutter A, (1968). Fish cells in culture for
study of aquatic toxicants. Water Res. 2(6), 409-414.
- 36. Roux F, (2015). Fish cell lines and their potential uses
in ecotoxicology: from cytotoxicity studies and mixture
assessment to a co-culture model and mechanistic
analyses. Master Thesis, Department of Biological
and Environmental Sciences University of Gothenburg,
Gothenburg.
- 37. Sanchez P, Llorente M.T, Castano A, (2000). Flow cytometric
detection of micronuclei and cell cycle alterations
in fish-derived cells after exposure to three model genotoxic
agents: mitomycin C, vincristine sulfate and benzo(a)
pyrene. Mutat Res. 465, 113–122.
- 38. Schirmer K, Dixon DG, Greenberg BM, Bols NC, (1998).
Ability of 16 priority PAHs to be directly cytotoxic to a cell
line from the rainbow trout gill. Toxicology. 127, 129–141.
- 39. Schirmer K, Chan AGJ, Bols NC, (2000). Transitory metabolic
disruption and cytotoxicity elicited by benzo[a]pyrene
in two cell lines from rainbow trout liver. J Biochem
Mol Toxicol. 14(5), 262-276.
- 40. Schirmer K, (2006). Proposal to improve vertebrate cell
cultures to establish them as substitutes for the regulatory
testing of chemicals and effluents using fish. Toxicology.
224, 163-183.
- 41. Schlenk D, Rice CD, (1998). Effect of zinc and cadmium
treatment on hydrogen peroxide-induced mortality and
expression of glutathione and metallothionein in a teleost
hepatoma cell line. Aquat Toxicol. 43, 121–129.
- 42. Tan F, Wang M, Wang W, Lu Y, (2008). Comparative evaluation
of the cytotoxicity sensitivity of six fish cell lines to
four heavy metals in vitro. Toxicol In Vitro. 22, 164-170.
- 43. Thibaut R, Porte C, (2008). Effects of fibrates, anti-inflammatory
drugs and antidepressants in the fish hepatoma
cell line PLHC-1: Cytotoxicity and interactions with cytochrome
P4501A. Toxicol in Vitro. 22, 1128–1135.
- 44. Wolf K, Quimby MC, (1962). Established eurythermic
line of fish cells in vitro. Science. 135(3508), 1065-10.