Türkiye’de gökkuşağı alabalığı çiftliklerinden izole edilen Listonella anguillarum suşlarının fenotipik özellikleri

Bu çalışmada Türkiye’de İç Anadolu ve Akdeniz bölgesinde bulunan 5 gökkuşağı alabalığı çiftliklerinde meydana gelen salgınlardan izole edilen 9 Listonella anguillarum izolatının fenotipik özellikleri belirlenmiştir. Enfekte gökkuşağı alabalıklarında letarji, deri renginde koyulaşma, bilateral ekzoftalmus, pullarda dökülme, vücut yüzeyinde furunkuller, deri, ağız, göz, yüzgeç ve anüste hemorajiler görülmüştür. İç bakıda asites, karaciğer, yağ doku ve kaslarda hemoraji, karaciğer ve böbrekte solgunluk, dalakta genişleme, mide ve bağırsakta sarı renkli kanlı sıvı birikimi tespit edilmiştir. Bakteriyolojik incelemeler için karaciğer ve böbrekten alınan örnekler, %1 NaCl ilave edilmiş Triptik Soy Agara ekilmiş ve 25°C de 48 saat süre ile inkübe edilmiştir. L.anguillarum izolatları (n=9) geleneksel bakteriyolojik yöntemler ve API 20 E kullanılarak identifiye edilmiştir. İzolatların fenotipik özelliklerinin homojen olduğutespit edilmiştir. Yapılan antibiyogram testinde tüm izolatların trimethoprim, tetrasiklin, oksitetrasiklin, enoksasin’e duyarlı ve flukloksasillin, sülfamethakzol, amoksisillin ve ampisilline karşı dirençli oldukları belirlenmiştir.

Phenotypic characteristics of Listonella anguillarum strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) farms in Turkey

In the present study, nine isolates of Listonella anguillarum were characterized isolated from epizootics at five rainbow trout fresh water farms in Middle and Mediterranean region of Turkey. Infected rainbow trout exhibited lethargy, dark skin, bilateral exophthalmos, spilling of scales, furuncles on the sides of the body, haemorrhage in the skin, mouth, eyes, fins and anus. Necropsy findings included haemorrhage in the liver, adipose tissue and muscles, pale kidney and liver, enlarged spleen, ascites in the body cavity, yellowish-bloody fluid in the stomach and intestine. Samples for bacteriological examinations were collected from the kidney and liver by using sterile swabs; these samples were streaked onto trypticase soy agar supplemented with 1% NaCl plates, and incubated at 25°C for 48 h. Nine bacterial isolates were obtained from sick fish. L.anguillarum isolates was identified by conventional methods and API 20 E. Phenotypic characteristics of the isolates were found as homogenous. All of L.anguillarum isolates were sensitive to trimethoprim, tetracycline, oxytetracycline, enoxacin and resistance to flucloxacillin, sulfamethoxazole, amoxicillin and ampicillin.

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