Şenay SEYİTOĞLU, Seyda CENGİZ, Serap KILIÇ ALTUN, Ömer Faruk KÜÇÜKKALEM, İbrahim SÖZDUTMAZ

Erzurum Yöresinde Koyunlarda Clostridium Perfringens Toksin Varlığının Toksin Nötralizasyon, ELI- SA ve Lateks Aglütinasyon Test Yöntemleri ile Araştırılması

Bu çalışmada, Erzurum yöresinde bulunan enterotoksemi şüpheli 70 adet koyuna ait bağırsak içeriğinin ELISA, toksin nötralizasyon ve lateks aglütinasyon testi (LAT) ile incelenerek; klostridial toksinlerin araştırılması ve tiplendirilmesi amaçlandı. Toksin testinde 25 (%35,7) örnekte C.perfringens yönünden pozitif sonuç alınırken, 45 (%64,3) adet örnek negatif olarak değerlendirildi. Bu test sonuçlarına göre, 14 örnek C.perfringens tip C, 2 örnek C.perfringens tip A, 9 örnek C.perfringens tip D olarak identifiye edildi. İncelenen örneklerin ELISA sonuçları değerlendirildiğinde 70 adet bağırsak içeriğinin 37 (%52,8) adeti C.perfringens yönünden pozitif bulunurken, 33 (%42,7) adet örnek negatif bulundu. Yapılan ELISA tiplendirilmesinde 12 adet numunede alfa toksin (tip A), 7 adet örnekte alfa, beta, epsilon toksin (tip B), 9 tanesinde alfa ve beta toksin (tip C) ve 9 adet örnek de alfa ve epsilon toksini (tip D) belirlendi. Lateks aglütinasyon sonuçlarında ise 2 örnek alfa toksin yönünden pozitif sonuç verirken, 16 örnekte epsilon toksini belirlendi. Sonuç olarak Erzurum ilinde enterotoksemi kontrolünde aşılamaların kontrollü, yaygın bir şekilde yapılmasının ve uygulanacak aşılarda başta tip A olmak üzere, tip C, tip D ile tip B’nin de bulundurulmasının gerekli olduğu belirlendi.

Anahtar Kelimeler:

Etlik Veteriner Mikrobiyoloji Dergisi, Enterotoksemi, Elisa, Lateks Aglütinasyon, Toksin Nötralizasyon, Şenay SEYİTOĞLU, Seyda CENGİZ, Serap KILIÇ ALTUN, Ömer Faruk KÜÇÜKKALEM, İbrahim SÖZDUTMAZ, 2012

Investigation of Clostridium Perfringens Toxins in Sheeps in the Region of Erzurum by Toxin Neutralization, ELISA and Lateks Agglütination Tests

Investigation and typing of clostridial toxins after ELISA, serum neutralization and latex agglutination tests (LAT) of intestinal contents from 70 enterotoxemia suspected sheeps in Erzurum region was aimed in this study. According to toxin neutralization results; 25 (35,7 %) and 45 (64,3 %) of the samples were determined as positive and negative, respectively. According to typing by toxin neutralization test; 14, 2 and 9 of the samples were typed as C, A and D, respectively. Thirty-seven (52,8 %) of the samples were positive for C.perfringens in the ELISA results. According to ELISA typing, 12 of the samples had alpha toxin (Type A), 7 of the samples had alpha, beta and gamma toxins (Type B), 9 of the samples had alpha and beta toxins (Type C), and 9 of the samples had alpha and epsilon toxins (Type D). According to latex agglutination test results, 2 of the samples had alpha and 16 of the samples had gamma toxins. As a result, the control of the province of Erzurum enterotoxemia, vaccination should be controlled and widely. Vaccines to be applied, especially in type A, type B, C and D must be present.

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