Sığırlardan izole edilen Mycobacterium bovis izolatlarının MIRU-VNTR analizi ile tiplendirilmesi

Sadece hayvan sağlığı değil aynı zamanda halk sağlığı açısından da önem arz eden sığır tüberkülozu, dünya genelinde yaygın olan zoonotik bir hastalıktır. Moleküler epidemiyolojinin ortaya çıkışıyla, hastalığın yayılma dinamiğinin anlaşılması ve daha etkili mücadele yöntemlerinin geliştirilebilmesinin temelleri atılmıştır. Bu çalışmada, bölgemizdeki Mycobacterium bovis izolatlarının genotip düzeyinde tanımlanması amaçlandı. Çalışmaya, Konya’daki kesimhanelerden alınan veya Konya Veteriner Kontrol Enstitüsü’ne teşhis için gönderilen tüberküloz şüpheli sığır doku ve organlarından klasik metotlarla izole edilen ve moleküler metotlar ile tanımlanan M. bovis suşlarının genotiplendirilmesi sonucu moleküler epidemiyolojik özellikleri belirlendi. Toplam 70 M. bovis izolatının, MIRU-VNTR yöntemi ile yapılan analizi sonucu; MIRU2, MIRU4, MIRU20, MIRU23, MIRU24, MIRU27 ve MIRU39 lokuslarındaki tekrar sayılarının suşlar arasında farklılık göstermediği, MIRU10, MIRU16, MIRU26, MIRU31 ve MIRU40 lokuslarındaki tekrar sayılarının ise suşlar arasında farklılık gösterdiği ve yüksek ayrım gücüne sahip olduğu (0.25≤h) ortaya kondu. Ayrıca izolat sayısı 1-14 arasında değişen 29 altgrup oluştuğu görüldü. Bölgemizde farklı sebepler ile yaşanan hayvan hareketliliğinin, M. bovis suşlarının sürüler arasında farklılık göstermesine, çalışmamızda ki izolatların kaynağı olan sığırların da farklı sürülere ait olmasının MIRU lokuslarında ayrım gücü çeşitliliğinin ortaya çıkmasına neden olduğu kanaatine varılmıştır.

Anahtar Kelimeler:

MIRU-VNTR, moleküler tiplendirme, Mycobacterium bovis, Sığır tüberkülozu

Typing of Mycobacterium bovis isolates from cattle using MIRU-VNTR analysis

Bovine tuberculosis, as a significant threat to both animal and human health, is a common global zoonotic disease. The emergence of molecular epidemiology has made it possible to gain a better understanding of the dynamics of disease transmission, and consequently, to come up with more effective control methods. The present study seeks to identify Mycobacterium bovis isolates in our region at a genotype level. To this end, the molecular epidemiological characteristics of Mycobacterium bovis strains isolated using classical methods and identified using molecular methods from the tissues and organs of cattle with suspected tuberculosis, obtained from slaughterhouses in the Konya province, or from those sent to Konya Veterinary Control Institute, were determined through genotyping. In the analysis of a total of 70 Mycobacterium bovis isolates, carried out using the MIRU-VNTR method, it was found that the repeat numbers for MIRU2, MIRU4, MIRU20, MIRU23, MIRU24, MIRU27 and MIRU39 loci did not vary between strains, while the repeat numbers for MIRU10, MIRU16, MIRU26, MIRU31, and MIRU40 loci varied between strains and had a high discriminatory power (0.25 ≤ h). It was further observed that 29 subgroups between 1-14 isolates formed. The movement of animals in our region, which occurs for several reasons, is considered to cause Mycobacterium bovis strains to vary between herds, and the fact that the cattle from which the isolates were sourced for our study came from different herds was considered to cause a variation in the discriminatory power in the MIRU loci.

Keywords:

bovine tuberculosis, MIRU-VNTR, molecular typing, Mycobacterium bovis,

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