Yüksek demir seviyeleri, oldukça ölümcül olan insan patojeni Vibrio vulnificus’un büyümesi ve virülansı ile güçlü bir şekilde ilişkilidir. Bu çalışma, farklı demir konsantrasyonları altında (7, 10, 15, 30 ve 50 μM) dört klinik ve dört çevresel V. vulnificus suşlarının, büyüme oranlarını ve hemolizin/sitolizin kodlayan genin (vvhA) ekspresyon seviyelerini incelemiştir. Ekspresyon seviyeleri 2-ΔΔCT yöntemine göre belirlenmiştir. Demir konsantrasyonu arttıkça vvhA transkripsiyon aşağı regülasyonu gözlenmiştir. Test edilen tüm suşlarda, 30 ve 50 uM ferrik klorür konsantrasyonları seviyesinde düşük konsantrasyonlara kıyasla anlamlı bir fark gözlenmiştir (p
Elevated levels of iron are strongly correlated with the growth and virulence of highly lethal human-pathogen Vibrio vulnificus. The present study examined the expression level of hemolysin/cytolysin-encoding gene (vvhA) and the growth rate of four clinical and four environmental strains of V. vulnificus under different iron concentrations (7, 10, 15, 30, and 50 μM). The expression levels of vvhA were determined according to the 2-ΔΔCT method. vvhA transcription was down-regulated as iron concentration increased. A significant difference was observed at the level of 30 and 50 μM ferric chloride concentrations compared to the lower concentrations in all the strains tested (p
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