Sığır Vebası Hastalığına Karşı Aşılanmış Analardan Doğan Buzağılardaki Maternal Antikorların, Competitive Elisa ile Kontrolu ve Buzağılarda İlk Aşılama Zamanının

Bu çalışmada aşılı analardan doğmuş ve kolostrum almış buzağılarda, sığır vebası materrial atikor varlığı .ve antikor seviyeleri, araştırılmış ve dolayısıyla doğum sonrası, ilk aşılamanın yapilabileceği en uygun dönemin belirlenmesi hedeflenmiştir. Bu amaçala 69 gebe sığırlardan, doğumdan önce serum alma zamanına bağlı olarak 1- 7 gün önce; 8-15 gün önce, 16-23 gun önce, 24-31 gün önce, 32-47 gün önce, 48-63 gün önce, 64-'94 gün önce ve üzeri; 69 buzağıdan da 1. aydan başlayarak 3. aya kadar birer aylık, 3 - 5 ay arasında 15 günlük periyotlarla ve 6.ayda kan serum örnekleri sağlanmıştır . Gebe sığırlardan ve bunlardan doğan buzağılardan periyodik aralıklarla alınan toplam 337 adet kan serum örneği RP C-ELISA ile test edilmiştir. Denemeye alınan 69 gebe sığır serum örneğinden, bir gebe sığır dışında diğer 68 gebe sığır serum örneğinde koruyucu düzeyde RPV antikoni bulunmuştur. Buzağılarda maternal antikor seviyelerinin yaşa bağlı olarak azalma· göstermesi ve 6.ayda denemeye alınan bütün buzağılarda maternal antikorların koruyucu seviyenin altına düşmesi nedeniyle, 6. aya kadar olan sürede kan serum örneği alınmıştır. Analarından kolostrum almış 69 buzağıdan 1. ayda 68 buzağıda, 2. ayda 68 buzağıdan 60 buzağıda, 3. ayda 60 buzağıdan 40 buzağıda, 3,5. ayda 40 buzağıdan 20 buzağıda, 4. ayda 20 buzağıdan 6 buzağıda, 4,5. ayda 6 buzağıdan 2 buzağıda ve 5. ayda 2 buzağıdan 1 buzağı- da koruyucu seviyede; 6. ayda ise 1 buzağıda koruyucu seviyenin altında antikor saptanmıştır. Elde edilen verilere göre; aşılı analardan doğan kolostrum almış buzağıların ilk 3,5 aylık sürede rriateı'nal arttikotlarla korunduğu ve bu buzağılara ilk aşının 3-3,5 aydan sonra yapılmasının uygun olacağı tespit edilmiştir.

Anahtar Kelimeler:

Arife ERTÜRK, İbrahim BURGU, maternal antikor, Sığır vebası, ELISA, Etlik Veteriner Mikrobiyoloji Dergisi

The Determination of the First Vaccination Time and the Control of the Ma ternal Antibodies Using Competitive Elisa of the Calves Born From the Mothers That Have Been Vaccinated Against Rinderpest Disease

In tliis study, the anti~ody levels and Rinderpest maternal antibody existance of the calves born from the vaccinated mothers and received the colostrum were investigated and therefore the d etermination of the most apprepricate vaccinate time was targeted. For this purpose, blood serum specimens were taken from 69 pregnant cattle ı-7 days before, ı6-23 days before, 24-3ı days before, 32-47days before, 48-63 daysor more before the parturition and from 69. caives beginning from the fırst month end of 6th month. Totally 337 blood sera Which were periodically collected from the pregnant cattle and the calves that had been born from these cattle were tested using RP-Competitive ELISA. Antibodies at the protective level against RPV were detected in 68 of the 69 blood sera of the pregnant cattle. Due to the facts that the maternal antibody levels decrease with the age and . the maternal aıi.tibody levels decline under the protictive level in all calves at the 6. month, the blöod sera specimens were collected in the period to the 6th month. The antibodies were detected under the protective level in 68 of the 69 calves which received the colostrum from the mothers at the ı st month, in 60 of the 68 calves at the 2nd month, in 40 of the 60 calves at the 3rd month, in 20 of the 40 calves the at the ı 05th day, in 6 of the 20 calves at the 4th month, 2 of the 6 calves at the ı35th day and ı of the 2 calves at the 5th month and in ı calfat the 6th month . . It was found from the data obtained that the calves born from vaccinated mothers and fed with colostrum from these mothers had been protected for 3.5 months (ı 05 days) against rinderpest by maternal antibodies. Therefore it was concluded . that th.e fırst vaccination to these calves should be applied after the age of 3 - 3.5 months.

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