Arife ERTÜRK, Nigar TATAR, Özden KABAKLI, Şerife (KOCABAŞ) İNÇOGLU, Ali İhsan AKIN

BVD-MD Yönünden Seronegatif Sığırlarda, Persiste BVD Enfeksiyonlarının Elisa İle Araştırılması

Türkiye'de daha önce farklı araştırıcılar tarafından bölgesel ve işletmeler düzeyinde yapılan çalışmalarda BVD enfeksiyonu prevalansının yüksek bulunması nedeniyle bu hastalığın ülkesel düzeyde araştırılmasının, hastalığın kontrolü ve mücadelesi açısından gerek. li oldugu gerçeğini ortaya koymuştur. Bu nedenle Türkiye genelinde yapılan bu araştırma sonuçları ile hastalığın kontrol ve mücadele kriterlerinin belirlenmesi ve hastalığın eradikasyonuna ışık tutması amaçlanmıştır. Bu çalışmada Türkiye genelinde ı505 adet sığır kan serum örneğinde% 60,2 seropozi~ · tiflik bulunmuş; BVD aşısı yapılmamış, alti aydan büyük hayvanlardaki bu seropozitiflik, hayvanların bu hastalığı geçirmiş olduklarını ya da immunotolerant olarak hastalığı taşıdıkları sonucunu ortaya koymaktadır. Bovine vinil diarrhoea (BVDV) enfeksiyonunun yayılmasında en büyük etken klinik belirti göstermeyen serenegatif olan persiste hayvanlardır. Bu amaçla serenegatif bulunan 599 adet hayvana ait defibrine kan örneği BVD Ag yönünden test edilmiş BVD antijen prevalansı % ı ,8 olarak bulunmuştur. · · Immunotolerant hayvanların tespiti amacıyla serepozitif bulunan 379 adet hayvandan 3 adeti antijen pozitif bulunmuş olup, BVD serenegatif ve serepozitif hayvanlarda antijen prevalansı %ı ,4 olarak tespit edilmiştir. BVD enfeksiyonunun Türkiye genelinde yaygın olduğu ve hastalığın mevcut sığır populasyonunda endemik hale dönüştüğü kanaatine varılmıştır. Çalışmada: BVD serepozitif- serenegatifhayvanları belirlemede BVD antikor ELlSA ve persiste h.ayvanların tespitinde de antijen ELISA kullanılmıştır.

Anahtar Kelimeler:

Arife ERTÜRK, Nigar TATAR, Özden KABAKLI, Şerife İNÇOGLU (KOCABAŞ), Ali İhsan AKIN, Türkiye, BVDV, seroloji, persiste enfeksiyon, ELISA, 2002, Etlik Veteriner Mikrobiyoloji Dergisi

The Investigation of Persistent BVD/MD Infections by ELISA in Cattle Who are Seronegative for BVD/MD

The prevalance of BVD infection had been found high in the studies carried out by a number of researchers at regionallevel or in certain farms. This was the reason to carry out a research at the base of country in order to direct the control of and combat with the disease. With this study conducted in the country as a whole to obtain the information and to set the criteria, necessary for the control of the disease and for i ts eradication was aimed. In this study, 60,2 % seropositivity was found in ı 505 cattle blood sera samples and that this seropositivity in the animals older than six months and not vaccinated indicated that the animals had already recovered or carried the disease as immunotolerant individuals. The most im portant factor in the transmission of bovine viral diarrhoea (BVDV) infection is persistently infected animals that do not show any elinical signs and are sero negative. Therefore, defibrinated blood samples which had been found seronegative from 599 animals were tested for BVD antigen and BVD antigen prevalance was found ı,8 %. In finding immunotolerant animal s, of 3 79 animal s which were seropositive, three were antigen positive and antigen prevalance was ı ,4 % in BVD seronegative and seropositive animals. It was concluded that BVD infection was wide spread in Türkiye and the disease was endemic in exsisting cattle population. In this study, BVD antibody ELISA and antigenELISA were used to find BVD seropositive- seronegative animals and persistently infected animals.

Keywords:

Türkiye, BVDV, serology, persiste infection, ELISA,

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