Yıldız AYAZ, Naim Deniz AYAZ, Mihriban AKSOY, Yusuf Ziya KAPLAN

Real-Time PCR Tekniği ile Çeşitli Et Ürünlerinde Tavuk ve Sığır Eti Oranlarının Kantitatif Tyini

Et ürünleri üretiminde kullanılan et türlerinin tür bazında ve oransal olarak doğru belirlenmesi tüketicinin aldatılmasının önlenmesi, halk sağlığının korunması ve üreticiler arası haksız rekabetin önlenmesi açısından büyük önem taşımaktadır.Yapılan bu çalışmada, hem tür analizi hem de kantitatif analiz yapabildiği üretici firma tarafından ifade edilen SureFood Animal QUANT Beef ve SureFood Animal QUANT Chicken real- time PCR kitleri kullanılmştır. Çalışmada deneysel olarak belirli oranlarda (%10 sığır eti + %90 tavuk eti, %20 sığır eti + %80 tavuk eti, %30 sığır eti + %70 tavuk eti, %50 sığır eti + %50 tavuk eti, %20 tavuk eti + %80 sığır eti, %30 tavuk eti + %70 sığır eti olmak üzere) sığır eti + tavuk eti karışımlarının yanı sıra farklı oranlarda sığır ve tavuk etinden hazırlanmış et ürünleri (salam, sosis ve sucuk) test materyali olarak kullanılmıştır. Et karışımlarından ve et ürünlerinden DNA ekstraksiyon işlemleri SureFood Prep Animal DNA ekstraksiyon kiti (Congen S 1003, Berlin, Almanya) ile yapılmıştır. DNA ekstraksiyonunu takiben Real-Time PCR tekniği ile et oranları tayin edilmeye çalışılmıştır. PCR analizleri Light Cycler-2 (Roche) cihazında gerçekleştirilmiştir. Çalışma sonuçlarına göre, kullanılan SureFood Animal QUANT Beef and SureFood Animal QUANT Chicken real-time PCR kitlerinin deneysel olarak farklı oranlarda sığır ve tavuk etinden hazırlanmış et karışımları ile yine çeşitli sığır ve tavuk eti karışımlarından yapılan salam, sosis ve sucuk örneklerinde sığır ve tavuk eti oranlarını kantitatif olarak doğru tespit edemediği ortaya konulmuştur.

Anahtar Kelimeler:

Etlik Veteriner Mikrobiyoloji Dergisi, Et Ürünleri, Et Türleri, Kantitatif Analiz, Real-Time PCR, Yıldız AYAZ, Naim Deniz AYAZ, Mihriban AKSOY, Yusuf Ziya KAPLAN, 2013

Quantitative Detection of Chicken and Beef Meat From Meat Products by Real-Time PCR

Quantitative detection of meat species that are used in production of meat products has a great importance for preventing consumers from adulteration, protection of public health and prevention of unfair competition between producers. In this study, SureFood Animal QUANT Beef and SureFood Animal QUANT Chicken real-time PCR kits were used to detect meat species quantitatively. For this purpose, various proportions (10% bovine + 90% chicken, 20% bovine + 80% chicken, 30% bovine + 70% chicken, 50% bovine + 50% chicken, 80% bovine + 20% chicken, 70% bovine + 30% chicken) of bovine and chicken meat mixtures and meat products (salami, sausage and sucuk from different bovine + chicken meat) were experimentally prepared. After DNA extraction process of these meat mixtures and products, Real-Time PCR technique was used for the quantitative detection of meat species. SureFood Prep Animal DNA extraction kit (Congen S 1003, Berlin, Germany) was used for the extraction of DNA from mixtures. PCR analyses were carried out with Light Cycler-2 (Roche, Germany). According to the real-time PCR results, SureFood Animal QUANT Beef and SureFood Animal QUANT Chicken kits were not able to detect the rates of meat species in meat mixtures (beef and chicken) and meat products (salami, sausage and sucuk) accurately.

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