Güzin İplikçioğlu ÇİL, F Seda Bilir ORMANCI

Piliç Boyun Derilerinden İzole Edilen C. perfringens'lerin Antibiyotik Direnç Profillerinin Belirlenmesi

Mikroorganizmaların antibiyotiklere karşı geliştirdikleri direnç, halen tüm dünyada önemini koruyan bir halk sağlığı sorunudur. Antibiyotik direnç ile mücadelede patojen mikroorganizmaların, gıdalarda güvenilir metotlarla tespiti, identifikasyonu ve antibiyotik direnç profillerinin belirlenmesi büyük önem taşımaktadır. Bu çalışmanın amacı, üç farklı kanatlı kesimhanesinden alınan 180 piliç boyun derisi örneğinden izole edilen 35 Clostridium perfringens suşunun antibiyotik direnç profillerinin belirlenmesidir. İzolatların antibiyotik direnç profillerinin tespitinde CLSI (Clinical and Laboratory Standards Institute) tarafından bildirilen disk difüzyon metodu kullanılmıştır. İzolatların 35'inin de (%100) en az bir antibiyotiğe dirençli olduğu, ayrıca 31 (% 88,5) C. perfringens izolatının 1'den fazla antibiyotiğe direnç gösterdiği tespit edilmiştir. İzolatların tamamının (% 100) dirençli olduğu antibiyotik trimetoprim olarak belirlenmiş, bu antibiyotik yanında, % 65,7 ile tetrasiklin, % 62,8 oranında gentamisin direncin yüksek oranda tespit edildiği diğer antibiyotikler olmuştur. Elde edilen veriler izolatların hepsinin vankomisine duyarlı olduğunu da ortaya koymuştur

Antibacterial Resistance of C. perfringens Isolated From Chicken Neck Samples

Antibacterial resistance is still an important public health problem, worldwide. Identification and detection of antibacterial resistance of pathogen microorganisms from foods are essential for prevention. The aim of this study was to detect the antibiotic resistance of 35 Clostridium perfringens, which are isolated from 180 chicken neck samples obtained from three slaughterhouses. For this purpose disc diffusion method was used according to the CLSI (Clinical and Laboratory Standards Institute). As a result, all (100 %) of the isolates were found to be resistant at least one of the 9 antibiotics also, 31 (88,5 %) of them were showed resistance to more than one antibiotic. 35 isolates was resistant to trimetoprim (100 %). In addition, high level of resistance was also determined against tetracycline (65,7 %) and gentamycin (62,8 %). All the isolates were found to be susceptible to vancomycin (100 %)

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