Öz Oxidative stress has been known to play an important role in the pathogenesis of various neurodegenerative diseases. Dietary polyphenols and other natural antioxidants are the most popular compounds in clinical testing for the elimination of neurodegeneration. It has been demonstrated in recent studies that fruit of Zizyphus jujuba possesses several vital biological activities. This study intends to evaluate antioxidant activity of Zizyphus jujuba on human glioblastoma cells. Cell survival was quantified by colorimetric viability assay with dose response. Cells were pretreated with 100 μM Ziziphus jujuba essential oil for 1h, then 100 μM H2O2 was added to the cells for 12 hours. After that, the cell medium were taken after treatment period and replaced with fresh medium. Total oxidant capacity (TOS) and total antioxidant capacity (TAS) levels were estimated using specific colorimetric methods. Oxidative stress index (OSI) was calculated from the ratio of TOS and TAS values. Many researches have been reported that the essential oil from seeds helps to prevent the oxidative stress induced neuronal diseases. The antioxidant potential of Ziziphus jujuba may be attributed to the presence of flavonoids and the other constituents present therein. Our data suggested that Ziziphus jujuba is effective in preventing H2O2-induced oxidative stress.
1. Adzu B, Amos S, Wambebe C, and Gamaniel K, (2001). Antinociceptive activity of Ziziphus spina christi root bark extract. Fitoterapia. 72, 344-350.
2. Cai YZ, Sun M, Corke H, (2003). Antioxidant activity of betalains from plants of the amaranthaceae. J Agric Food Chem. 51(8), 2288-2294.
3. Castellani RJ, Zhu X, Lee HG, Smith MA and Perry G, (2009). “Molecular pathogenesis of Alzheimer’s disease: reductionist versus expansionist approaches,” International Journal of Molecular Sciences. 10(3), 1386-1406.
4. Chen J, Maiwulanjiang M, Lam KY, Zhang WL, Zhan JY, Lam CT, Xu SL, Zhu KY, Yao P, Lau DT, Dong TT, Tsim KW, (2014). A standardized extract of the fruit of Ziziphus jujuba (Jujuba) induces neuronal differentiation of cultured PC12 cells: a signaling mediated by proteinkinase. A. J Agric Food Chem. 62, 1890-1897.
5. Cheng G, Bai Y, Zzhao Y, Tao J, Liu Y, Tu G, Ma L, Liao N, Xu X, (2000). Flavonoids from Ziziphus jujuba Mill var. Spinosa. Tetrahadron, 56(45), 8915-8920.
6. Choi SH, Ahn JB, Kozukue N, Levin CE, Friedman M, (2011). Distribution of Free Amino Acids, Flavonoids, Total Phenolics, and Antioxidative Activities of Jujuba (Ziziphus jujuba) Fruits and Seeds Harvested from Plants Grown in Korea. J Agric Food Chem. 59(12), 6594-6604.
7. Emerit J, Edeas M, Bricaire F, (2004). Neurodegenerative diseases and oxidative stress. Biomed Pharmacother. 58, 39-46.
8. Erel OA, (2005). New colorimetric method for measuring total oxidant status. Clin Biochem. 38(12):1103-11.
9. Floyd RA, Hensley K, (2002). Oxidative stress in brain aging. Implications for therapeutics of neurodegenerative diseases. Neurobiol Aging. 23(5), 795-807.
10. Gao QH, Wu PT, Liu JR, Parry JW, Wang M, (2011). Physico-chemical properties and antioxidant capacity of different jujuba (Ziziphus jujuba Mill.) cultivars grown in loess plateau of China. Scientia Horticulturae. 130(1), 67- 72.
11. Guil-Guerrero JL, Diaz Delgado A, Gonzalez MCM, and Torija Isasa ME, (2004). Fatty acids and carotenes in some ber (Ziziphus jujuba mill) varieties. Plant Foods Hum Nutr, 59, 23-27.
12. Gupta RB, Sharma S, Sharma JR, and Goyal R, (2004). Study on the physicochemical characters of fruits of some wild and cultivated forms/spp. (Zizpi hus spp.). Haryana Journal of Horticultural Sciences. 33(3/4), 167-169.
13. Hansen MB, Nielsen SE, Berg K, (1989). Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. Journal of Immunology Methods. 119, 203-210.
14. Huang YL, Yen GC, Sheu F, Chau CF, (2008). Effects of water-soluble carbohydrate concentrate from Chinese jujuba on different intestinal and fecal indices. J Agric Food Chem. 56, 1734-9.
15. Kaeidi A, Taati M, Hajializadeh Z, Jahandari F, Rashidipour M, (2015). Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy. Iran J Basic Med Sci. 18, 301-306.
16. Kandimalla R, Dash S, Kalita S, Choudhury B, Malampati S, Kalita K, Kalita B, Devi R, Kotoky J, (2016). Protective Effect of Bioactivity Guided Fractions of Ziziphus jujuba Mill. Root Bark against Hepatic Injury and Chronic Inflammation via Inhibiting Inflammatory Markers and Oxidative Stress. Front. Pharmacol. 7, 298.
17. Kim MC, Cui FJ, Kim Y, (2013). Hydrogen Peroxide Promotes Epithelial to Mesenchymal Transition and Stemness in Human Malignant Mesothelioma Cells. Asian Pacific Journal of Cancer Prevention. 14(6), 3625-3630.
18. Kitamura Y, Ota T, Matsuoka Y, Tooyama I, Kimura H, Shimohama S, Nomura Y, Gebicke-Haerter PJ, Taniguchi T, (1999). Hydrogen peroxide-induced apoptosis mediated by p53 protein in glial cells. Glia. 25(2), 154-164.
19. Klaunig JE, Kamendulis LM, (2004). The role of oxidative stress in carcinogenesis. Annu Rev Pharmacol Toxicol. 44, 239-67.
20. Kumar S, Ganachari MS, Banappa, V, Nagoor S, (2004). Anti-inflammatory activity of Ziziphus jujuba Lam leaves extract in rats. Journal of Natural Remedies, 4(2), 183-185.
21. Mahajan RT, Chopda MZ, (2009). Phyto-Pharmacology of Ziziphus jujuba Mill-A plant review. Phcog Rev. 3(6), 320- 329.
22. Mark PM, (2004). Metal-catalyzed disruption of membrane protein and lipid signaling in the pathogenesis of neurodegenerative Disorders. Ann NY Acad Sci. 1012, 37-50.
23. Mitra A, and Dey B, (2013). “Therapeutic interventions in Alzheimer disease,” in Neurodegenerative Diseases. InTech, Rijeka, chapter 12, 291-317.
24. Park JH, Lee HJ, Koh SB, Ban JY, (2004). Protection of NMDA-induced neuronal cell damage by methanol extract of Zizyphi Spinosi Semen in cultured rat cerebellar granule cells. Journal of Ethnopharmacology, 95 (1), 39-45.
25. Pawlowska AM, Camangi F, Bader A, and Braca A, (2009). Flavonoids of Zizyphus jujuba L. and Zizyphus spina-christi (L.) wild (Rhamnaceae) fruits. Food Chem. 112, 858-862.
26. Poon HF, Calabrese V, Scapagnini G, Butterfield DA, (2004). Free radicals: key to brain aging and heme oxygenase as a cellular response to oxidative stress. J Gerontol A Biol Sci Med Sci. 59(5), 478-93.
27. Salganik RI, (2001). The benefits and hazards of antioxidants: controlling apoptosis and other protective mechanisms in cancer patients and the human population. J Am Coll Nutr. 20, 464-72.
28. Taatil M, Alirezaei M, Meshkatalsadat MH, Rasoulian B, Moghadasi M, Sheikhzadeh F, Sokhtezari A, (2011). Protective effects of Ziziphus jujuba fruit extract against ethanol-induced hippocampal oxidative stress and spatial memory impairment in rats. Journal of Medicinal Plant Research. 5(6), 915-921.
29. Tavakkoli M, Miri R, Jassbi AR, Erfani N, Asadollahi M, Ghasemi M, Saso L, Firuzi O, (2014). Salvia and Stachys species protect neuronal cells against oxidative stress-induced apoptosis. Pharm Biol. 52(12), 1550-7.
30. Zheng W, Wang SY, (2001). Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem. 49 (11), 5165-70.