Normal ve Hepatitisli Tavuklardan Campylobacter İzolasyonu Üzerinde Çalışmalar

Bu araştırmada selektif izolasyon yöntemleri kullanılarak, termofilik Campylobacter türlerinin tavuk karaciğerlerinde yoğunluğunun belirlenmesi amaçlanmıştır. Etlik Hayvan Hastalıkları Araştırma Enstitüsü Tavuk hastalıkları teşhis laboratuvarına gelen yumurtacı ırklara ait tavuk karaciğerleri ile mezbahalardan toplanan broiler karaciğerlerden eküvyonla alınan örnekler modifiye preston selektif besiyerine ekildi. Miroaerofilik koşullarda 42°C'de 48 saat inkübasyona bırakıldı. Bu sürenin sonunda üreyen mikroorganizmalar Campylobacter türleri yönünden üreme ve biyokimyasal testler ile incelendiler. ldentifiye edilen termatilik Campylobacter türleri iki farklı sistem ile biyotiplendirildiler. Incelenen 4 broiler sürüsünün 3'ünde, laboratuvarımıza gelen 124 adet yumurtacı tavuğun 5'inde Campylobacter türleri saptandı. Kesim periyodundaki 7-9 haftalık breilerlerin % 45'inden Campylobacter izolasyonu yapılmıştır. Bunun% 44.1'i C.coli,% 55.8'i C.jejuni şeklinde idenlifiye edildi. Tavuk karaciğerlerinde C.laridis ise bulunamadı. Lior biyotiplendirme sistemine göre izole edilen C.jejuni suşları 3 biyotipe, C.coli suşları 2 biyotipe ayrıldı. Suşların çoğunun biyotip l'de toplandığı tespit edildi. Skirrow-Benjamin biyotiplenirme sistemine göre 2 biyotipe ayrılan C.jejuni suşlarının çoğunun biyotip l'e girdiği saptandı.

Anahtar Kelimeler:

Tavuk, Hepatitisli Tavuk, Campylobacter, Campylobacter İzolasyonu, Hepatitisli Tavuklardan Campylobacter İzolasyonu, Fatma KOÇ, 1992, Etlik Veteriner Mikrobiyoloji Dergisi

«Studies on isolation of Campylobacter from normal chickens and chickens with hepatitis»

This study was aimed to calculate concentration of the thermophlic Campylobacter species in chicken liver by selective isolation. Liver samples collected by swab from layer which were referrals to Etlik Animal Disease Rescearch lnst. Lab. and which were collected from slaughter house. Samples taken from liver were cultured by modificated Preston selective and incubated at 42°C in microaerophilic atmosphere for 48 hours. The multiplied microorganism were examined by using growth and biochemical tests. lsolated species of thermophilic Campylobacter were biotyped by two different biotyping systems. Campylobacter species were identified in a broiler flock of 3 out of 4, layer 5 out of 124 referrals in the Iab. In the of slaughtering broilers that aged were 7-9 weeks, Campylobacter was isolated at a percentage of 45 %, 44.1 % was C.coli and 55.8 % was identified as C.jejuni, C.laridis coulddn't identified in chicken liver. C.jejuni strains which were isolated by "Lior Biotyping system" had 3 biotypes and C.coli strains had 2 biotypes. Most of strains from all two species accumulated into biotype 1 of each species. In addition, C.jejuni strains were classified as biotype 1 (most of strains) and biotype 2 by using biotyping scheme of Skirrow-Benjamin.

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