Fluorescent antikor metodu

Immunochemichal boyama usullerinin tekamülü hakkında kısk'hir tarihçe ile bu metodun teşhisteki önemi takdim edilmiştir. Fluorescent antikor testlerinin bulaşık hastalıklann teşhis vekontrolu ile ilgili olarak mikrobiyoloji sahasında gelecekteki önemimünakaşa edildi.Fluoı'escense'ın miktar ve tabiatı ile optik aletlerin seleksiyonundanbahsedildLKullanılan fluorescent antikor testlerinin tipleri açıklandı veFA metodunun daha yavaş ve fazla laboratuvar çalışmasını icabettirenstandart testlere nazaran mikroorganizmlerin sür'atle idantifikasyonundaki büyük önemi belirtildi.Bilinmeyen antijen ve antikodann FA metodu ile çabuk ve özelolarak idantifikasyonlan münakaşa edildi.

Anahtar Kelimeler:

Fluorescent antikor

Fluoreseent antibody technique

A short history of the development of the ImmunochemichaJstaining procedures and the imporlance of this method in diagnosishave been presented.The future significance of the fluorescent antibody tests in microbiologyin connection with the diagnosis and control of the contagiousdiseases was discussed.The nature and quantity of fluorescence and the selection ofthe optical equipment have been discussed.The types of fluorescent antibody tests which are in use havebeen explained. The importance of the FA method in the rapid identificationof microorganisms was pointed out as opposed to the slowstandard tests which require a great amount of laboratory work.The rapid and spesific way of identifying unknown antigenand antibody through the FA method has been discussed.

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1 - Cherry, W. E., Goldman, M., and Carsld, T. R., with the collaboration ot Moody, M. (1960) Fluorescent Antibody Techniques in the Diagnosis of communicable diseases. United States Government Printlng office Washintong D. C. Public Health Service Publication No. 729.
2 - Communicable Disease Center Laboratory Branch Atlanta, Georgia. Training Course in Fluorescent Antibody Technique for Grouping Beta Hemolytic Steptococci. Lal:ıor:.ıtory Manual. (1961).
3 Coons A. H., and Kaplan, M. H. : Localization of antigen in tissuc cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody J. Exper. "Med. 91 : 1 - 13, 1950.
4 Cunningham, Charles H. (1960) A laboratory Guide in Virology.
5 Kaplan, M. H., Coons, A. H. and Deane, H. W. : Localization of Antigen in Tissue Cells. III - Celluler distribution of pneumococcal polysaccharides typesii and III in the mouse. J. Exp. Med. 91 : 15 - 30, 1950.
6 - Goldwasser R. A. and Shepard C .C. : Sta.ining of complement and modifications o! fluorescent antibody procedures. J. Immuno!. 80 : 122 - 131, 1958.
7 Moody, Max D .. Ellis, E. C. and Updyke, E. L. : Statining Bacterial Smears with Fluorescent antibody. VI - Ideni!ication of Salmonellae In Fecal Spccimens (REPRİNT)
8 - Pelczar, M. J. Jr. ,Reid, R. D. (1958) Microbiology.