HepG2 hücre hattının canlılığı üzerine medium pH'sının etkisinin incelenmesi

Kanser hücreleri, tümör mikroçevresinin asitleşmesine neden olan artmış glikoz alımı ve laktat üretimi ile karakterizedir. Asidik mikro-çevre kanser hücrelerinin invazyon ve metastaz gelişimini kolaylaştırır. Bu çalışmada medium pH değişiminin, in vitro insan hepatoselüler karsinom (HepG2) hücrelerinin proliferasyon ve canlılığına etkisinin değerlendirilmesi amaçlanmıştır. HepG2 hücreleri pH değeri 6.0 ila 8.5 arasında değişen medium ortamlarında 24 saat boyunca inkübe edildi. Daha sonra hücre canlılığı ve proliferasyon değerlendirmeleri için sırasıyla WST-1 ve tripan mavisi hücre canlılık testleri uygulandı. HepG2 hücre canlılığında, medium pH’sı 6.6 ve 6.8 olan gruplar ile kontrol grubu (pH 7.2) karşılaştırıldığında önemli bir farkın bulunmadığı (p> 0.05), ancak pH 6.4, 6.2, 6.0'da önemli oranda azalmanın olduğu saptandı (p

An evaluation of the effects of medium pH on the viability of the HepG2 cell line

Cancer cells are characterized by increased glucose uptake and the production of lactate, which leads to acidification of the tumor microenvironment. This acidification facilitates the development of invasiveness and metastasis. In this study, we investigated the effects of medium pH manipulation on the proliferation and viability of human hepatocellular carcinoma (HepG2) cells in vitro. HepG2 cells were grown in media with pH ranging from 6.0 to 8.5 for 24 h. The cells were then subjected to WST-1 and trypan blue exclusion assays to evaluate viability and cell proliferation, respectively. At pH 6.8 and 6.6, HepG2 cell viability was not significantly different from the control group (pH 7.2) (p>0.05) but there was a significant decrease at pH 6.4, 6.2 and 6.0 (p < 0.05). Furthermore, there was a significant decrease in cell viability at pH’s 7.8, 8.0 and 8.5 (p0.05), and decreased at pH 7.6 and 7.8 (significant at pH 7.8; p

Kaynakça

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Kaynak Göster

APA Güvenalp, N , Güvenç, D . (2020). An evaluation of the effects of medium pH on the viability of the HepG2 cell line . Etlik Veteriner Mikrobiyoloji Dergisi , 31 (2) , 107-114 . DOI: 10.35864/evmd.742482
  • ISSN: 1016-3573
  • Yayın Aralığı: Yılda 2 Sayı
  • Yayıncı: Veteriner Kontrol Merkez Araştırma Enstitüsü

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